Ekspresi Protein p53 Mutan dan Ki-67 pada Kondiloma Akuminata dan Karsinoma Sel Skuamosa

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Agustin Nurliani
Willy Sandhika

Abstract

ABSTRAK

Latar belakang
Diagnosis kondiloma akuminata (KA) dan karsinoma sel skuamosa (KSS) dapat ditegakkan berdasarkan pemeriksaan histopatologi. Sebagian besar kasus gambaran mikroskopik kondiloma akuminata dan karsinoma sel skuamosa berbeda nyata. Pada kasus tertentu seperti pada karsinoma sel skuamosa tipe verrucous dapat menyerupai kondiloma akuminata. Beberapa penelitian telah dikembangkan untuk membantu penegakan diagnosis dengan melihat ekspresi p53 mutan dan Ki-67 pada lesi-lesi tersebut. Tujuan penelitian ini untuk mengetahui ekspresi protein p53 mutan dan Ki-67 dalam diagnosis kondiloma akuminata dan karsinoma sel skuamosa.
Metode
Penelitian yang dilakukan menggunakan desain cross sectional. Populasi penelitian adalah blok parafin dari sediaan kulit dengan diagnosis kondiloma akuminata, karsinoma sel skuamosa diferensiasi baik atau tipe verrucous yang diarsipkan di Laboratorium Patologi Anatomik RSUD Dr. Soetomo periode Januari 2010-Desember 2012. Sampel penelitian terdiri dari 15 kasus KA dan KSS dari seluruh kasus yang ditemukan. Sampel dipulas menggunakan pulasan imunohistokimia dengan antibodi primer p53 dan Ki-67. Ekspresi p53 mutan dan Ki-67 dinilai berdasarkan jumlah inti sel yang tercat dalam 100 sel. Perbedaan ekspresi p53 mutan dan Ki-67 pada KA dan KSS dianalisis dengan menggunakan uji statistik.
Hasil
Dari 15 kasus KA rerata usia penderita 36,07+12,30 tahun, dan 15 kasus KSS rerata usia penderita 49,93+15,65 tahun. Hasil uji statistik menunjukkan perbedaan yang tidak bermakna ekspresi protein p53 mutan pada KA dan KSS (p=0,131). Terdapat perbedaan yang bermakna ekspresi protein Ki-67 pada KA dan KSS (p=0,001).
Kesimpulan
Ekspresi protein Ki-67 dapat membedakan kondiloma akuminata dengan karsinoma sel skuamosa. Ekspresi protein p53 mutan tidak dapat membedakan kondiloma akuminata dengan karsinoma sel skuamosa.
Kata kunci : karsinoma sel skuamosa, Ki-67, kondiloma akuminata, p53 mutan.

ABSTRACT

Background
The diagnosis of condyloma accuminata (CA) and squamous cell carcinoma (SCC) can be made based on histopathological examination. In most cases the microscopic picture of condyloma accuminata and squamous cell carcinoma clearly different. However, in certain cases such as SCC, verrucous type can form a picture that resembles condyloma accuminata. Several studies have been developed to assist in the diagnosis by looking at the expression of p53 mutant and Ki-67 proteins in these lesions. To analyze the differences in the expression of p53 mutant and Ki-67 proteins in condyloma accuminata and squamous cell carcinoma.
Methods
Analytical observational research with cross sectional approach. Population of this study was paraffin blocks from skin preparation with a diagnosis of condyloma accuminata and squamous cell carcinoma well differentiated and squamous cell carcinoma verrucous type stored in the Anatomical Pathology Laboratory Hospital Dr. Soetomo within the period January 2010 to December 2012. The samples used in this study were taken, respectively-each of the 15 cases from all cases were found. Paraffin blocks that met the criteria were stained with immunohistochemical method using antibody p53 and Ki-67. The degree of expression of p53 mutant and Ki-67 were assessed by quantity of cells was staining in 100 cells. Differences in the expression of p53 mutant and Ki-67 in condyloma accuminata and squamous cell carcinoma were analyzed using statistical tests.
Results
15 cases of condyloma accuminata mean patient age 36.07 +12.30 years, and 15 cases of squamous cell carcinoma patients mean age 49.93 +15.65 years. The test results showed statistically no significancy in the expression of p53 mutant protein both condyloma accuminata and squamosa cell carcinoma (p=0.131). There is a significant in the expression of Ki-67 in condyloma accuminata and squamosa cell carcinoma (p=0.001).
Conclusion
Expression Ki-67 protein play a role in distinguishing condyloma accuminata with squamous cell carcinoma. Expression of protein p53 mutant protein do not play a role in differentiating condyloma accuminata with squamosa cell carcinoma. There was no relationship between the expression of protein p53 and Ki67 in condyloma accuminata and squamous cell carcinoma.
Key words : condyloma accuminata, Ki-67, p53 mutant, squamous cell carcinoma.

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Author Biographies

Agustin Nurliani

Departemen Patologi Anatomik, Fakultas Kedokteran, Universitas Airlangga Surabaya

Willy Sandhika

Departemen Patologi Anatomik, Fakultas Kedokteran, Universitas Airlangga Surabaya