Relationship between Tumor-Associated-Macrophages and Tumor-Associated-Neutrophils Density with Clinicohistopathological Characteristics of Hodgkin's Lymphoma Patients

Abstract

Background
The development and aggressiveness of tumor cells were influenced by tumor microenvironment. Tumor-Associated-Macrophages
(TAMs) and Tumor-Associated-Neutrophils (TANs) are components of tumor microenvironment, contribute to development and growth
of tumor cells. The aim of this research was to analyse correlation of TAMs and TANs densities with clinicohistopathologic
characteristics of patients i.e. age, gender, location, and subtype.
Methods
A case series was conducted. The samples were paraffin blocks of Hodgkin lymphoma patients at Anatomic Pathology Department
Faculty of Medicine Universitas Sriwijaya/RSUP Dr. Mohammad Hoesin Palembang. Among 44 cases of Hodgkin lymphoma
diagnosed from 1stJanuari 2015 to 30th October 2019, only 31 cases were included. Immunohistochemistry was performed by using
anti-CD163 antibody and neutrophils were identified by morphologic appearances. The results were correlated to characteristic
factors. Statistical analysis was performed using Spearman and Lambda test with significant consideration if p<0.05.
Results
The study results showed 30 cases of classical Hodgkin lymphoma (CHL) and 1 case of NLPHL. CHL has only 3 variants LR
(lymphocyte-rich) (60%), MC (mixed-cellularity) (26.7%), and NS (nodular sclerosis) (13.3%). Hodgkin lymphoma is most commonly
found in males (61.3%). Tumor were mostly found in the head and neck, and patient ages were majority in the age group 20-29 years,
40-49 years, and over 50 years. Spearman test showed there was a significant correlation between density of TAMs with ages
(p=0.043).
Conclusion
High density of TAM was correlated with age, however, there was no correlation between TAN and clinicohistopathologic
characteristics of Hodgkin lymphoma.