2026-06-16T02:01:27Z http://majalahpatologiindonesia.com/p/index.php/patologi/oai

oai:ojs.pkp.sfu.ca:article/381 2022-04-12T05:44:15Z patologi:ART driver

Hubungan antara Ekspresi E-cadherine dan Tipe Histopatologik Tumor Ganas Epitel Ovarium Hera Novianti, R Zuryati Nizar Aswiyanti Asri Kata kunci: ekspresi E-cadherine, tipe histopatologik, tumor ganas epitel ovarium. Latar belakangTumor ganas epitel ovarium merupakan keganasan ginekologi kedua terbanyak di dunia dan menjadi penyebabkematian kelima akibat keganasan, bersifat proliferatif intra abdomen dengan invasi dan metatasis yangseringkali mengakibatkan kegagalan terapi. Tumor ganas epitel ovarium diklasifikasikan menjadi tipe I dan II.Tipe II dengan prognosis yang lebih buruk dan lebih banyak bermetastasis. Salah satu protein transmembranyang memiliki peran penting dalam proses metastasis dan invasi tumor adalah E-cadherine. Ekspresi Ecadherine yang menurun ditemukan pada berbagai tumor ganas namun peran E-cadherine pada tumor ganasepitel ovarium masih dalam perdebatan. Penelitian ini bertujuan untuk menganalisis hubungan antara ekspresiE-cadherine dan tipe histopatologik tumor ganas epitel ovarium.MetodeTigapuluh dua kasus tumor ganas epitel ovarium yang telah didiagnosis, dikumpulkan dari beberapalaboratorium Patologi Anatomik di kota Padang. Pada seluruh sampel dilakukan review slaid HE untukmenetapkan tipe histopatologik, dilanjutkan dengan pewarnaan imunohistokimia menggunakan E-cadherin.Ekspresi E-cadherine positif ditandai dengan warna coklat pada membran sel tumor. Hubungan antar variabeldianalisis dengan uji statistik chi-square dengan nilai p<0,05 dianggap bermaknaHasilEkspresi E-cadherine positif ditemukan pada 22 kasus, yaitu pada 12 kasus tumor ganas epitel tipe I dan 10kasus tumor ganas epitel tipe II, p=0,704 (p>0,05). Tidak terdapat hubungan bermakna antara ekspresi Ecadherine dengan tipe histopatologik tumor ganas epitel ovarium.KesimpulanEkspresi E-cadherine dan tipe I histopatologik tumor ganas epitel ovarium menunjukkan hubungan positif. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2019-05-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/381 10.55816/mpi.v28i2.381 Majalah Patologi Indonesia; Vol. 28 No. 2 (2019): MPI; 1 - 5 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/381/262

oai:ojs.pkp.sfu.ca:article/390 2022-04-14T05:01:44Z patologi:ART driver

Korelasi Ekspresi MMP-9 dengan Derajat Histologik dan Faktor Karakteristik Derajat Histologik pada Karsinoma Payudara Invasif Simon, Yolanda Isabela Susraini, A.A.A.N Moestikaningsih histological grade, invasive breast carcinoma, MMP-9. Kata kunci : derajat histologik, karsinoma payudara invasif, MMP-9 BackgroundHistological grade and MMP-9 expression are prognostic factors in invasive breast carcinoma, nevertheless thecorrelation between both prognostic factors is still debatable. The aim of this study was to determine therelationship between expression of MMP-9 and histological grade and to evaluate which characteristic factor ofthe histological grade is dominant to MMP-9 expression in invasive breast carcinoma.MethodsThis study was carried out using a cross sectional analytical method. The samples were paraffin blocks frominvasive carcinoma of no special type grade I, II, and III at Pathology Anatomy Department UdayanaUniversity/RSUP Sanglah Denpasar from 1st January 2013 to 31st July 2016. Histopathologically re-diagnosiswas conducted to get the eligible samples and obtained 47 samples, consisted of 7 samples grade I, 18samples grade II, and 22 samples grade III. Immunohistochemistry of the MMP-9 was done and the result wascorrelated to histological grade and characteristic factor of histological grade.ResultsThe study results showed high expression of MMP-9 2.1% (n=1) in grade I, 19.2% (n=9) in grade II, and 36.2%(n=17) in grade III. Chi-square test analysis showed there was a significant correlation between tumor gradeand MMP-9 expression (p=0,010). The logistic regression test showed the mitotic counting was statisticallysignificant dominant factor (p=0.012) determining MMP-9 expression, whereas tubular formation and nuclearpleomorfia were not statistically significant (p>0.05).ConclusionHigh expression of MMP-9 was correlated with high histological grade, which also indicated worse prognosis.MMP-9 testing is an important examination to determine the level of tumour aggresive-ness and plan theeffective therapies ABSTRAKLatar belakangDerajat histologik dan ekspresi MMP-9 pada karsinoma payudara invasif tipe tidak spesifik merupakan faktorprognosis, namun hubungan antara derajat histologik dengan ekspresi MMP-9 masih menimbulkan perdebatan.Tujuan penelitian ini untuk membuktikan adanya hubungan antara ekspresi MMP-9 dengan derajat histologikdan untuk mengetahui faktor karakteristik derajat histologik yang dominan terhadap ekspresi MMP-9 padakarsinoma payudara.MetodePenelitian ini menggunakan metode analitik potong lintang. Sampel penelitian adalah sediaan blok parafinkarsinoma payudara invasif tipe tidak spesifik derajat histologik I, II, dan III di Bagian/SMF Patologi AnatomikFakultas Kedokteran Universitas Udayana/RSUP Sanglah Denpasar sejak tanggal 1 Januari 2013 sampaidengan 31 Juli 2016. Dilakukan rediagnosis pada 47 sampel yang memenuhi kriteria inklusi dan eksklusi yangterdiri dari 7 kasus derajat histologik I, 18 kasus derajat histologik II, dan 22 kasus derajat histologik III.Dilakukan pulasan imunohistokimia untuk MMP-9, kemudian ekspresinya dihubungkan dengan derajathistologik dan masing-masing karakteristik derajat histologik.HasilEkspresi MMP-9 pada derajat histologik I 2,1% (n=1), pada derajat histologik II 19,2% (n=9), dan pada derajathistologik III 36,2% (n=17). Uji Chi-square menunjukkan perbedaan bermakna (p=0,010) antara derajathistologik dengan ekspresi MMP-9. Uji regresi logistik menunjukkan hasil yang bermakna adalah hitung mitosis(p=0,012), sedangkan formasi tubular dan pleomorfik inti tidak bermakna secara statistik (p>0,05).KesimpulanEkspresi MMP-9 yang tinggi berhubungan dengan derajat histologik yang tinggi sehingga perlu dilakukanpemeriksaan ekspresi MMP-9 untuk menentukan tingkat agresivitas tumor dan merencanakan terapi yangefektif. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2019-09-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/390 Majalah Patologi Indonesia; Vol. 28 No. 3 (2019): MPI; 1 - 6 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/390/270

oai:ojs.pkp.sfu.ca:article/391 2022-04-14T05:03:16Z patologi:ART driver

Hubungan Ekspresi Reseptor Progesteron dan Ki-67 Labeling Index dengan Derajat Histopatologik Meningioma Sri Rejeki, Dwi Agus, Salmiah Syafrita, Yuliarni histopathological degree of meningioma, Ki-67 LI, progesterone receptor derajat histopatologik meningioma, Ki-67 LI, reseptor progesteron BackgroundMeningioma is tumor originating from meningothelial cells (arachnoid) attached to duramater. These is thesecond most tumor in central nervous system. All degrees meningioma have risk for recurrence, unfortunatelythe biologic behavior of meningioma can’t be seen from histopathology alone. Therefore, many studies haveshown progesterone receptor influence the degree of meningioma and Ki-67 LI also has important role indetermining risk of recurrence in the degree meningioma. Therefore, the examination of progesterone receptorand Ki-67 LI in meningioma is required to see character of meningioma tumor cells and hence to determinetreatment and prognosis.MethodsThe study was a cross-sectional study, which conducted patients with diagnosis of meningioma from 5Pathological Anatomy Laboratories in West Sumatra. Total of 64 cases meningioma were obtained duringJanuary 2012 to December 2015 and 35 samples were taken and met the inclusion criteria.Immunohistochemical examination was using specific antibodies to progesterone receptor and Ki-67. Datacollected were analyzed using the Kruskal-Wallis test and p value <0.05 was considered as statisticallysignificant.ResultsFrom 35 samples, 29 samples (82.9%) classified meningioma grade I, 4 samples (11.4%) meningioma grade IIand 2 samples (5.7%) meningioma grade III. Meningioma was most prevalent the age range 41-50 years,female gender and histopathological grade I.ConclusionNo differences expression progesterone receptor on the degree histopathological meningioma. No differencesexpression Ki-67 LI on the degree histopathological meningioma. There is inverse relationship betweenexpression progesterone receptor and Ki-67 LI on the degree histopathological meningioma ABSTRAKLatar belakangMeningioma adalah tumor yang berasal dari sel meningotel (arachnoid) yang menempel pada duramater.Tumor ini merupakan tumor terbanyak kedua pada susunan saraf pusat, terbanyak pada perempuan. Semuaderajat meningioma berisiko untuk terjadi rekurensi, sayangnya perilaku biologik dari meningioma tidak dapatdilihat hanya dari gambaran histopatologik saja. Pada banyak penelitian jenis tumor lain menunjukkan bahwareseptor progesteron mempengaruhi derajat histologik. Dikatakan Ki-67 LI berperan penting dalam menentukanrisiko rekurensi pada derajat histologik meningioma. Dengan demikian maka pada meningioma diperlukanpemeriksaan reseptor progesteron dan Ki-67 LI untuk melihat perangai dari sel tumor yang dapat menentukanterapi dan prognosis.MetodePenelitian menggunakan metode cross-sectional study. Sampel penelitian ini penderita meningioma di 5Laboratorium Patologi Anatomik Sumatra Barat. Didapatkan sebanyak 64 kasus meningioma didapatkanselama Januari 2012 sampai Desember 2015, dan 35 sampel diambil yang memenuhi kriteria inklusi. Dilakukanpemeriksaan imunohistokimia menggunakan antibodi yang spesifik dengan reseptor progesteron dan Ki-67.Analisis statistik menggunakan uji Kruskal-Wallis Test dan dianggap bermakna bila p<0,05.HasilSebanyak 35 sampel, 29 sampel (82,9%) dikelompokkan pada meningioma derajat I, 4 sampel (11,4%)dikelompokkan pada meningioma derajat II dan 2 sampel (5,7%) dikelompokkan pada meningioma derajat III.Meningioma paling banyak ditemukan pada kelompok usia 41-50 tahun, jenis kelamin perempuan dan derajathistopatologik derajat I.KesimpulanTidak terdapat perbedaan ekspresi reseptor progesteron pada derajat histopatologik meningioma. Tidakterdapat perbedaan ekspresi Ki-67 LI pada derajat histopatologik meningioma. Terdapat hubungan terbalikantara ekspresi reseptor progesteron dan Ki-67 LI pada derajat histopatologik meningioma. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2019-09-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/391 Majalah Patologi Indonesia; Vol. 28 No. 3 (2019): MPI; 7 - 13 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/391/271

oai:ojs.pkp.sfu.ca:article/392 2022-04-14T05:05:00Z patologi:ART driver

Hubungan Jumlah Sel Mast dengan Tampilan Ekspresi Vascular Endothelial Growth Factor (VEGF) pada Cutaneous Squamous Cell Carcinoma Sembiring,, Edi Kerina Delyuzar Soekimin cutaneous squamous cell carcinoma, mast cell, VEGF. sel mast, squamous cell carcinoma, VEGF. BackgroundThe most common non-melanocytic skin cancers are basalioma and squamous cell carcinoma. Squamouscell carcinoma is a malignancy of squamous epithelium. Mast cells stimulate neovascularization andangiogenesis in multiple tumors. One of the main factors of angiogenesis in squamous cell carcinoma isthe Vascular Endothelial Growth Factor (VEGF). The aim of this study is to investigate the associationbetween number of mast cells with the expression of VEGF of cutaneous squamous cell carcinoma.MethodsThis is an analytical descriptive study with cross sectional approach. The sample of this study are paraffinblocks of skin tissue which diagnose histopathologically as squamous cell carcinoma. Then the sampleswill be stained with immunohistochemistry CD117 (Novocastra®) to count the number of mast cells andVEGF (Dako®) to see angiogenesis. Their expression will be analyzed with Fisher’s Exact Test statisticalanalysis to investigate the association between number of mast cells with expression of VEGF ofcutaneous squamous cell carcinoma.ResultsThe number of mast cells ≥15 cells were 7 cases (23.3%). Expressed VEGF positive expression in 30cases (100%). Suitability test Fischer's Exact Test has value not significant (p> 0.05).ConclusionThere is no conformance relationship between the number of mast cells with VEGF expression of p-value. ABSTRAKLatar belakangKanker kulit non melanositik tersering adalah basalioma diikuti squamous cell carcinoma. Squamous cellcarcinoma merupakan keganasan epitel skuamus. Sel mast merangsang neovaskularisasi danangiogenesis pada beberapa tumor. Salah satu faktor utama angiogenesis pada squamous cell carcinomaadalah vascular endothelial growth factor (VEGF). Tujuan penelitian ini untuk mengetahui hubunganjumlah sel mast dan ekpresi vascular endothelial growth factor (VEGF) pada cutaneous squamous cellcarcinoma.MetodePenelitian ini merupakan penelitian deskriptif analitik dengan pendekatan secara cross sectional. Sampelpenelitian adalah blok parafin yang berasal dari jaringan kulit yang didiagnosis secara histopatologiksebagai squamous cell carcinoma. Kemudian sampel dipulas dengan imunohistokimia CD117(Novocastra®) untuk menilai jumlah sel mast dan VEGF (Dako®) untuk menilai angiogenesis. EkspresiVEGF akan dianalisis dengan uji statistik Fisher’s Exact Test untuk melihat hubungannya dengan jumlahsel mast pada cutaneous squamous cell carcinoma.HasilJumlah Sel mast ≥15 sel sebanyak 7 kasus (23,3%). Ekspresi VEGF terekspresi positif pada 30 kasus(100%). Uji kesesuian Fischer’s Exact Test memiliki nilai yang tidak signifikan (p>0,05).KesimpulanTidak terdapat kesesuaian hubungan jumlah sel mast dengan ekspresi VEGF dari p-value Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2019-09-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/392 Majalah Patologi Indonesia; Vol. 28 No. 3 (2019): MPI; 14 - 21 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/392/272

oai:ojs.pkp.sfu.ca:article/393 2022-04-14T05:07:01Z patologi:ART driver

Hubungan Ekspresi Ki-67 dan E-Cadherin dengan Kedalaman Invasi Melanoma Malignum Berdasarkan Clark Level Saberko, Santy Agus, Salmiah Yenny, Satya Wydya malignant melanoma, Ki-67, E-Cadherin, depth of invasion based on Clark level melanoma malignum, Ki-67, E-Cadherin, kedalaman invasi berdasarkan Clark level. BackgroundMalignant melanoma (MM) is a neoplasia derived from melanocytes. This is the most aggressive cancer ofskin. Even small tumors may have a tendency to metastasize and thus lead to a relatively unfavorableprognosis. Malignant melanoma depth of invasion based on Clark level is one of the important prognosticfactors to determine survival rate of MM patient. Determination of invasion based on this Clark level issubjective, more objective checks such as Ki-67 and E-Cadherin are required. This examination isexpected to see the risk of metastasis in MM. Cell proliferation is the main key for tumor progression and itcan be measured with Ki-67 expression. E-cadherin is one of transmembrane protein that plays importantrole in metastasis. The purpose of this study is to determine correlation between Ki-67 and E-Cadherinexpression with depth of invasion of malignant melanoma based on Clark level.MethodsThis is cross sectional study in Padang and Jakarta, with 42 cases of malignant melanoma which havebeen diagnosed in Pathology Anatomic Laboratory in West Sumatera. Samples were obtained fromparaffin block of or tissue from surgery and have been reviewed depth of invasion based on the Clark level.These samples were divided into I-V Clark level group. Ki-67 expression of cells nuclei and expression ofE-Cadherin of cells membrane were analyzed using immunohistochemistry assay. One Way Annovabivariate statistical analysis were used and value p<0.05 were considered significant.ResultsPositive Ki-67 expression were found in 42 cases, 6 samples with II Clark level, 8 samples with III Clarklevel, 19 samples with IV Clark level, 9 samples with V Clark level. Malignant melanomas were mostlyfound in male 60-79 age group and depth of invasion in IV Clark level. Positive E-Cadherin expression in IIClark level. There was significant correlation between Ki-67 and E-Cadherin  ABSTRAKLatar belakangMelanoma malignum (MM) adalah keganasan yang berasal dari sistem melanosit kulit. Tumor inimerupakan tumor paling agresif dari semua keganasan kulit. Tumor dengan ukuran kecil dapat mengalamiinvasi dan metastasis dengan prognosis buruk. Kedalaman invasi MM berdasarkan Clark level merupakansalah satu faktor prognosis penting untuk menentukan ketahanan hidup pasien. Penentuan invasiberdasarkan Clark level ini bersifat subjektif maka diperlukan pemeriksaan yang lebih objektif sepertipemeriksaan imuhistokimia Ki-67 dan E-cadherin. Pemeriksaan ini diharapkan mampu melihat risikometastasis pada MM. Proliferasi sel merupakan kunci utama progresi tumor dan dapat diukur denganpemeriksaan ekspresi Ki-67. E-Cadherin merupakan salah satu protein transmembran yang memiliki peranpenting dalam proses metastasis tumor. Tujuan penelitian ini untuk menilai ekspresi Ki-67 dan E-Cadherinpada kedalaman invasi MM berdasarkan Clark level HasilEkspresi Ki-67 positif ditemukan pada 42 kasus di mana rerata ekspresi Ki-67 pada Clark level II yaitu12,17%, Clark level III 39,38%, Clark level IV 57,63% dan Clark level V 64,44%. Ekspresi E-Cadherinpositif pada Clark level II sedangkan pada Clark level III, IV dan V sebagian besar ekspresi E-Cadherinnegatif. Melanoma malignum paling banyak ditemukan pada kelompok usia 60-79 tahun, jenis kelaminlaki-laki, subtipe histopatologik nodular melanoma dan kedalaman invasi Clark level IV. Terdapathubungan bermakna antara ekspresi Ki-67 dan E-Cadherin dengan kedalaman invasi berdasarkan Clarklevel p=0,001.KesimpulanEkspresi Ki-67 berbanding lurus dan ekspresi E-Cadherin berbanding terbalik dengan kedalaman invasimelanoma malignum berdasarkan Clark level. Ekspresi Ki-67 dan E-Cadherin mampu memprediksi risikometastasis pada melanoma malignum. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2019-09-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/393 Majalah Patologi Indonesia; Vol. 28 No. 3 (2019): MPI; 22 - 28 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/393/273

oai:ojs.pkp.sfu.ca:article/394 2022-04-14T05:08:14Z patologi:ART driver

Ekspresi Bcl-2 pada Karsinoma Urotelial Kandung Kemih dan Hubungannya dengan Berbagai Faktor Prognosis Yudhanto, Hendy Setyo Tanurahardja, Budiana Wuyung , Puspita Eka urothelial carcinoma bladder, degree of malignancy, stage, mitosis, limfovaskuler invasion, and Bcl-2 expression. Kata kunci: derajat keganasan, ekspresi Bcl-2, invasi limfovaskuler, karsinoma urotelial, mitosis, stadium. BackgroundUrothelial carcinoma comprises of 90% of all cases in bladder. There are two groups which are non invasivedepend on whether the tumor has reached muscularis mucosa. 5-Years survival rate depend on grading andstaging. Low malignant grade and non invasive may reach 90% survival rate, with recurrence rate 40-60%. Highmalignant grade and invasive tumor has only10-17% survival rate. Mitosis and lymphovascular invasion arerelated with recurrency. However, there are some controvesi regarding Bcl-2 expression in bladder urothelialcarcinoma. This study was aimed to evaluate different expression of Bcl-2 with 4 related factors contributed tosurvival, which are degree of malignancy, stage, mitosis, and lymphovascular invasion.MethodsA retrospective cross sectional study on urothelial carcinoma of the bladder was conducted in the Departmentof Anatomical Pathology, Faculty of Medicine, University of Indonesia/RSCM. The cases were collected fromthe year of 2010 to 2014. In this study 21 cases of low grade and 21 cases of high high grade were found. Bcl-2immunohistochemistry staining performed in all cases and percentages are calculated to make 0-3 scoring.ResultsMost case is fifth decade as many as 27 cases. Obtained 4 cases found limfovaskuler invasion. This studyobtains the relationship between mitosis with grade of tumor (p: 0.000). the result suggests that there are nodifferences between the expression of Bcl-2 with the degree of malignancy stage, mitosis and lymphovascularinvasion. ABSTRAKLatar belakangKarsinoma urotelial kandung kemih merupakan kasus terbanyak di organ kandung kemih mencapai 90% kasus.Stadium dibedakan menjadi 2 kelompok yaitu non invasif jika belum menembus lapisan muskularis dan invasifjika sudah menembus lapisan muskularis. Kesintasan 5 tahun tergantung dari derajat keganasan dan stadium.Derajat keganasan rendah dan belum invasi muskuler dapat mencapai 90%, tetapi angka rekurensi berkisar40-60%. Derajat keganasan tinggi dan sudah invasi hanya berkisar 10-17%. Mitosis dan invasi limfovaskulerberhubungan dengan angka rekurensi tinggi. Namun masih terdapat kontroversial terhadap ekspresi Bcl-2 padakarsinoma urotelial kandung kemih. Penelitian ini bertujuan untuk menilai perbedaan ekspresi Bcl-2 dengan 4faktor yang berhubungan dengan prognosis yaitu derajat keganasan, stadium, mitosis, dan invasi limfovaskuler.MetodeDilakukan penelitian deskriptif analitik secara potong lintang pada karsinoma urotelial kandung kemih tahun2010-2014 di Departemen Patologi Anatomik FKUI/RSCM. Didapatkan 21 kasus derajat keganasan rendah dan21 kasus derajat keganasan tinggi. Dilakukan pulasan Bcl-2 pada seluruh kasus dan dihitung persentasenyadan dilakukan skoring 0-3.HasilUsia terbanyak pada dekade 5 sebanyak 27 kasus. Didapatkan 4 kasus ditemukan invasi limfovaskuler.Penelitian ini mendapatkanhubungan antara mitosis dengan derajat keganasan (p:0,00) dengan koefisienkorelasi 0,512 Penelitian ini mendapatkan hampir seluruh kasus mengekspresikan Bcl-2 (39 dari 42 kasus), 1kasus tidak mengekspresikan dan 2 kasus mengekpresikan sedikit. Tidak didapatkan perbedaan antaraekspresi Bcl-2 dengan derajat keganasan (p:0,232), stadium (p:0,455), mitosis (p:0,835), dan invasilimfovaskuler (p:0,087). Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2019-09-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/394 Majalah Patologi Indonesia; Vol. 28 No. 3 (2019): MPI; 29 - 37 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/394/274

oai:ojs.pkp.sfu.ca:article/395 2022-04-14T05:10:30Z patologi:ART driver

Hubungan Ekspresi Matriks Metaloproteinase-9 dengan Jenis Histopatologi Karsinoma Serviks dan Invasi Limfovaskular Oktora, Meta Agus, Salmiah Sulaini, Pelsi adenocarcinoma, LVI, MMP-9, squamous cell carcinoma (SCC). adenokarsinoma, karsinoma sel skuamosa (KSS), LVI, MMP-9. BackgroundMatrix Metalloproteinase 9 (MMP-9) is a proteolytic enzyme assumed to have an important role in cervicalcarcinogenesis. Limfovascular invasion (LVI) and higher expression of MMP-9 will make cervical tissuedegradation faster, invasion process and metastasist easier. Adenocarcinoma is suspected of having poorprognostic than squamous cell carcinoma (SCC). The aim of this study was to examine the relationshipbetween expression of MMP-9 with histopathological cervical carcinoma and LVI.MethodsA cross sectional analytic study was performed on 36 sample that divided into 18 KSS and 18 adenokarsinoma.Samples were tooken from block paraffin from surgery or biopsy in Anatomic Pathology laboratories are locatedin West Sumatra. Sample are reviewed and found the LVI. Immunohistochemical was staining with primaryantibody MMP-9 then its expression were assessed in stroma and cytoplasmic cell tumour.ResultsMMP-9 expression was found positive in 80.6% (41.1% for SCC and 58.6% for cervical adenocarcinoma. Themean of expression of SCC was 17.4 and cervical adenocarsinoma was 19.56. Expression of MMP-9 waspositive in LVI sample.ConclusionThere is not significant relationship between the expression of MMP-9 with type of histopatologic cervicalcarcinoma (p=0.088) and LVI (p=0.566). Expression of MMP-9 in cervical adenocarcinoma was higher thanSCC, but it was not significant (p=0.563). ABSTRAKLatar belakangMetriks metaloproteinase 9 (MMP-9) merupakan enzim proteolitik yang diduga berperan penting dalamprogresivitas suatu keganasan khususnya pada serviks. Adanya invasi limfovaskular (LVI) dan ekspresi MMP-9yang tinggi akan menyebabkan proses degradasi jaringan serviks menjadi lebih cepat dan mempermudahproses metastasis. Jenis adenokarsinoma diduga memiliki prognostik yang buruk dibanding karsinoma selskuamosa (KSS). Penelitian ini bertujuan untuk melihat persentase ekspresi MMP-9 dan hubungannya denganjenis histopatologik karsinoma serviks dan invasi tumor limfovaskular.MetodePenelitian ini menggunakan metode analitik potong lintang. Sampel pada penelitian ini sebanyak 36 kasus yangterdiri atas 18 sampel KSS dan 18 sampel adenokarsinoma. Sampel diperoleh dari blok parafin yang berasaldari tindakan operasi atau biopsi di laboratorium Patologi Anatomik yang berada di Sumatera Barat. Sediaan direview dan dinilai invasi tumor limfovaskular. Pewarnaan imunohistokimia dilakukan dengan antibodi primerMMP-9, kemudian ekspresinya dinilai pada stroma dan sitoplasma sel.HasilEkspresi MMP-9 ditemukan positif sebanyak 80,6% pada semua sampel, di mana 41,1% pada KSS dan 58,6%pada adenokarsinoma serviks. Rerata ekspresi MMP-9 pada KSS 17,4 dan pada adenokarsinoma 19,56.Ekspresi MMP-9 positif pada semua sampel dengan LVI.KesimpulanTidak terdapat hubungan ekspresi MMP-9 dengan jenis histopatologi karsinoma serviks (p=0,088) dan LVI(p=0,566). Rata-rata ekspresi MMP-9 tinggi pada jenis adenokarsinoma, tetapi secara statistik tidak terdapatperbedaan yang bermakna (p=0,563). Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2019-09-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/395 Majalah Patologi Indonesia; Vol. 28 No. 3 (2019): MPI; 38 - 44 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/395/275

oai:ojs.pkp.sfu.ca:article/396 2022-04-14T05:11:47Z patologi:ART driver

Akurasi Pemeriksaan Polymerase Chain Reaction Menggunakan Primer IS6110 dan MPB64 untuk Mendeteksi Mycobacterium tuberculosis pada Spesimen Formalin-Fixed Paraffin-Embedded Djitro, Timotius Benedict Nur Izzaty, Faramitha Rahadiani, Nur FFPE, IS6110, MPB64, mycobacterium tuberculosis, paraffin, polymerase chain reaction : FFPE, IS6110, MPB64, mycobacterium tuberculosis, parafin, polymerase chain reaction BackgroundOne of the chalangges faced in controlling tuberculosis (TB) is the availability of a fast and precise diagnostictool. The current gold standard for diagnosis of TB is acid-fast bacterial culture. However, FFPE (formalin-fixedparaffin-embedded) tissues are not ammenable for culture and the time required makes this method impracticalfor many centers. PCR is an alternative rapid diagnostic tool, and can be used with FFPE tissues. PCR hasshown high sensitivity and specificity for detecting M.tuberculosis, however there has been reports of lesssensitivity and specificity when using FFPE specimens. This report aims to determine the accuracy of PCRdetection of M.tuberculosis using primer IS6110 and MPB64 on FFPE specimens.MethodsLiterature searches were carried out on the Pubmed, Scopus, Proquest, Springer Link and Cochranedatabases. Two journals were relevant to the clinical scenario, then a critical review is carreid out using theDiagnostic Study Appraisal Worksheet from the Center for Evidence-based Medicine, University of Oxford,2010.ResultsCurrently, the accuracy of PCR primer IS6110 and MPB is not satisfactory. Sensitivity ranges from 45.5-88.9%and specificity is between 88.1% - 100. This broad sensitivity range is probably caused by tissue volume andembedding process that can cause damages to the integrity of M.tuberculosis DNA.ConclusionPCR examinations using IS6110 and MPB64 primers can be used as confirmation tests in FFPE specimensdue to its high specificity value. Broad sensitivity values can produce significant false negatives if no otherexamination is used in conjunction with PCR in FFPE specimens. ABSTRAKLatar belakangTantangan yang dihadapi dalam mengontrol penyakit tuberculosis (TB) adalah ketersediaan alat diagnostikyang cepat dan tepat. Pemeriksaan baku emas untuk diagnosis TB saat ini adalah kultur bakteri tahan asam,tetapi metode ini membutuhkan waktu yang lama dan tidak dapat digunakan pada jaringan yang telah difiksasidengan formalin. Pemeriksaan PCR memliki sensitivitas dan spesifisitas yang tinggi, membutuhkan waktu yanglebih singkat dibanding kultur, serta dapat digunakan pada jaringan yang telah difiksasi dengan formalin.Laporan ini bertujuan mengetahui akurasi pemeriksaan PCR menggunakan primer IS6110 dan MPB64 untukmendeteksi M.tuberculosis pada spesimen FFPE (formalin-fixed paraffin-embedded).MetodeDilakukan pencarian literatur pada basis data Pubmed, Scopus, Proquest, Springer Link dan Cochrane.Terdapat dua jurnal yang relevan dengan kasus, lalu dilakukan telaah kritis menggunakan Diagnostic StudyAppraisal Worksheet dari Centre for Evidence-based Medicine, University of Oxford, 2010.HasilDari kedua jurnal, akurasi PCR dengan primer IS6110 dan MPB64 saat ini masih kurang baik, Sensitivitasberkisar antara 45,5-88,9%, spesifisitas antara 88,1-100%. Rentang nilai sensitivitas yang luas ini kemungkinandisebabkan oleh volume dan proses embedding jaringan yang dapat menyebabkan kerusakan integritas DNAM.tuberculosis.KesimpulanPemeriksaan PCR dengan primer IS6110 dan MPB64 dapat digunakan sebagai konfirmasi pada spesimenFFPE karena nilai spesifisitas yang tinggi dari pemeriksaan ini. Nilai sensitivitas yang bervariasi berisikomenghasilkan negatif palsu apabila tidak ada pemeriksaan lain yang digunakan bersama dengan PCR padaspesimen FFPE. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2019-09-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/396 Majalah Patologi Indonesia; Vol. 28 No. 3 (2019): MPI; 45 - 51 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/396/276

oai:ojs.pkp.sfu.ca:article/397 2022-04-14T05:13:39Z patologi:ART driver

Hubungan Ekspresi Imunohistokimia Cyclooxygenase-2 (COX-2) dengan Derajat Histopatologi Meningioma Mahrani, Indri Delyuzar S. Lukito, Joko cyclooxygenase-2, histophatologic grading, immunohistochemistry, meningioma derajat histopatologik, cyclooxygenase-2, meningioma, imunohistokima. BackgroundMeningioma is the most common intracranial primary neoplasm in adults, which arise from arachnoid cap(meningothelial) cells. Histological appearance of a meningioma is an important predictor of tumor behavior.Cyclooxygenase-2 (COX-2) enzyme is overexpressed in different types of tumors and is known to beassociated with malignant behavior of tumors. The aim of this study was to evaluate cyclooxygenase-2 (COX-2)immunoreactivity in meningioma and to find association with histopathologic grading of meningioma.MethodsThis is an analytical descriptive study with cross sectional approach. The histopathologic grading of 22 samplesof meningioma were determined and stained with COX-2 immunohistochemistry. The color intensity: 1 (mild), 2(moderate), 3 (strong). Percentage of positive cells: 0 (0%), 1 (<10%), 2 (10-50%), 3 (>50%). The evaluationwas the sum of color intensity and percentage of positive cells: positive (>4), negative (<4).ResultsTwnty two cases of meningioma: mean age=41 years, 90.9% females, meningothelial=22.7%, grade I=77.3%).COX-2 expression: negative: 17 cases (77,3%) all was first degree of malignancy. COX-2 positive: 5 cases(22,7%): 4 cases of second degree and 1 case of third degree of malignancy. The expression of COX-2 inmeningioma cells was associated with WHO histopathologic grading (p=0.001).ConclusionThis study showed a relationship between COX-2 immunohistochemical expression and histopathologic gradingof meningioma, where COX-2 was more expressed in grades II and III. ABSTRAKLatar belakangMeningioma adalah neoplasma intrakranial primer yang umum pada orang dewasa, berasal dari sel arachnoid(meningothelial). Gambaran histologis meningioma adalah prediktor penting untuk perilaku tumor.Cyclooxygenase-2 (COX-2) adalah enzim yang diekspresikan dalam berbagai jenis tumor dan diketahui terkaitdengan perilaku ganas tumor. Tujuan dari penelitian ini untuk mengevaluasi ekspresi imunohistokimia COX-2pada meningioma dan mencari hubungannya dengan derajat histopatologik.MetodePenelitian Ini merupakan penelitian deskriptif analitik dengan pendekatan cross sectional. Sampel penelitian iniadalah 22 blok parafin dengan diagnosis meningioma yang ditentukan derajat histopatologiknya dan diwarnaidengan imunohistokimia COX-2. Penilaian dilihat berdasarkan intensitas warna dan persentase sel yangterwarnai pada sitoplasma. Intensitas warna: 1 (lemah), 2 (sedang), 3 (kuat). Persentasi sel yang positif=0(0%), 1 (<10%), 2 (10-50%), 3 (>50%). Penilaian merupakan penjumlahan intensitas warna dan persentasipositif (>4), negatif (<4).HasilDuapuluh dua kasus meningioma: usia rerata=41 tahun, perempuan=90,9%, meningothelial=22,7%, derajatI=77,3%). Ekspresinya. COX-2: negatif: 17 kasus (77,3%) semuanya merupakan derajat I. COX-2 positif: 5kasus (22,7%) (4 kasus derajat II, 1 kasus derajat III). Ekspresi COX-2 pada meningioma berhubungan denganderajat histopatologik berdasarkan WHO (p=0,001).KesimpulanPada penelitian ini terdapat hubungan antara ekspresi imunohistokimia COX-2 dengan derajat histopatologik, dimana COX-2 lebih terekspresi pada derajat II dan III. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2019-09-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/397 Majalah Patologi Indonesia; Vol. 28 No. 3 (2019): MPI; 52 - 57 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/397/277

oai:ojs.pkp.sfu.ca:article/401 2025-12-06T00:58:32Z patologi:ART driver

Thyroid Aspiration Overview in the Diagnosis of Suspicious Malignancy (Thyroid Papillary Carcinoma) Gambaran Sitologi Aspirasi Tiroid pada Diagnosis Curiga Keganasan (Karsinoma Papiler Tiroid) Chendrasari, Julian Rahmiati Hamdani, Chairil FNAB thyroid, suspicious for malignancy (SFM), cytological feature, papillary thyroid carcinoma (PTC). BAJaH tiroid, curiga keganasan, gambaran sitologi, karsinoma papiler tiroid (KPT). BackgroundThyroid carcinoma is the most common malignancy in the endocrine system. Fine Needle Aspiration Biopsy (FNAB) is a preliminaryexamination to detect thyroid neoplasms. One of FNAB diagnostic category according to Bethesda is Suspicious for Malignancy(SFM). This study was conducted to assess the cytology feature of SFM category and its concordance with histopathologic result.MethodsThis retrospective study is a descriptive categoric study with cross sectional design of 40 samples. Secondary datas were arsip inlaboratory of Anatomical Pathology of FKUI/RSCM collected on 2015 diagnosa as suspect for malignancy of tiroid based onhistopathologic result.ResultsThe histopathological results of SFM were: PTC 20 cases (50%), Hurthle cell carcinoma 2 cases (5%), adenomatous goitre withPTC focus 11 cases (27%), adenomatous goitre with atypical cell 1 case (2%), adenomatous goitre 4 cases (10%), follicularadenoma 1 case (3%), Hashimoto's thyroiditis with atypical cell 1 case (3%). Positive predictive value was 82.5% with 94% ofmalignancies were papillary type. Cytology feature of SFM specimen: cellularity moderate (57.5%), macrofollicular (67.5%), nuclearchange powdery chromatin (85%). Giant cell were 18 cases (45%, cytoplasmic were 20 cases (50%), bubble gum colloid were, 9cases (22.5%).ConclusionMost of SFM specimen have malignant histopathologic result, especially papillary type. The positive predictive value of the SFMcategory is quite high, but there is still disconcordance on histopathologic results. Latar belakangKarsinoma tiroid merupakan keganasan tersering pada sistem endokrin. Biopsi Aspirasi Jarum Halus (BAJaH) merupakanpemeriksaan awal untuk mendeteksi karsinoma tiroid. Salah satu kategori diagnostik BAJaH menurut Bethesda adalah curigakeganasan. Telaah ini dilakukan untuk menilai gambaran sitologi kategori curiga keganasan dan kesesuaiannya dengan hasilhistopatologik.MetodeTelaah retrospektif ini merupakan penelitian deskriptif kategorik dengan desain potong lintang pada 40 sampel. Data sekunderberasal dari arsip Departemen Patologi Anatomi FKUI/RSCM tahun 2015 dengan diagnosis sitologi curiga keganasan pada tiroidyang memiliki data histopatologik.HasilHasil histopatologik curiga keganasan: KPT 20 kasus (50%), karsinoma sel Hurthle 2 kasus (5%), struma adenomatosa disertaifokus KPT 11 kasus (27%), struma adenomatosa disertai sel atipik 1 kasus (2%), struma adenomatosa 4 kasus (10%), adenomafolikuler 1 kasus (3%), tiroiditis Hashimoto disertai sel atipik 1 kasus (3%). Nilai duga positif 82,5% dengan 94% keganasan jenispapiler. Gambaran sitologi sediaan curiga keganasan: selularitas sedang (57,5%), struktur makrofolikular (67,5%), perubahan intikromatin powdery (85%). Sel datia 18 kasus (45%), sitoplasma 20 kasus (50%), dan koloid bubble gum 9 kasus (22,5%).KesimpulanSebagian besar sediaan curiga keganasan memiliki diagnosis histopatologik ganas terutama jenis papiler. Nilai duga positif kategoricuriga keganasan cukup tinggi, tetapi masih ditemukan ketidaksesuaian dengan hasil histopatologik. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/401 10.55816/mpi.v29i1.401 Majalah Patologi Indonesia; Vol. 29 No. 1 (2020): MPI; 1 - 7 2527-9106 0215-7284 10.55816/mpi.v29i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/401/278

oai:ojs.pkp.sfu.ca:article/402 2025-12-06T00:58:32Z patologi:ART driver

Relationship of CD117 Expression and Epidermal Growth Factor Receptor (EGFR) to Histopathological Grading of Adenoid Cystic Carcinoma in Salivary Glands Hubungan Ekspresi CD117 dan Epidermal Growth Factor Receptor (EGFR) terhadap Grading Histopatologi Adenoid Cystic Carcinoma pada Kelenjar Liur Siregar, Ren Astrid Allail Alferraly, T. Ibnu Delyuzar adenoid cystic carcinoma, CD117, EGFR adenoid cystic carcinoma, CD117, EGFR BackgroundTend to have adenoid cystic carcinoma (AdCC) is one of malignant epithelial tumor of the salivary gland poor prognosis, localrecurrence and metastases. Histopathological grading system according Weert, et al. observed from presence or absence solidcomponent of AdCC. CD117 is a transmembrane tyrosine kinase receptor usually strong expression in AdCC. EGFR belongs to theErbB family of receptor tyrosine kinases associated with cell proliferation, angiogenesis and metastases. Patients with advanceddisease may benefit from targeted therapy besides conventional management. Expression of CD117 and EGFR may give proposaltherapy through difference histology pattern of AdCC based on histopathological grading.MethodsThis is an analytical descriptive study with cross sectional approach. Formalin-fixed paraffin-embedded sections from 35 adenoidcystic carcinomas were obtained for histopathological grading and for immunostained of CD117 and EGFR using monoclonalantibodies. The staining intensity and percentage of cells with membranous and cytoplasmic of CD117 and EGFR expression werescored and then grouped according to the parameters of the Allred scoring system.ResultsChi-square test between CD117, EGFR expression and histopathological grading of AdCC showed no sigficant differences.Besides, McNemar test between CD117 and EGFR expression and histopathological grading AdCC showed significant differences,p-value=0.03 (p<0.05).ConclusionsCD117 and EGFR could be used to detected salivary gland AdCC Latar belakangAdenoid cystic carcinoma (AdCC) merupakan salah satu keganasan epitel kelenjar liur yang cebderung memiliki prognosis buruk,rekurensi lokal dan metastasis. Sistem grading histopatologi AdCC berdasarkan Weert, et al. dinilai dengan menentukan ada atautidaknya komponen solid. CD117 adalah suatu reseptor tirosin kinase transmembran yang sering dilaporkan terekspresi kuat padaAdCC. EGFR merupakan salah satu anggota keluarga reseptor tirosin kinase yang terkait pada proliferasi sel, angiogenesis danmetastasis. Pasien AdCC dengan stadium lanjut memiliki keuntungan dari manfaat targeted therapy selain denganpenatalaksanaan konvensional. Ekspresi CD117 dan EGFR diharapkan dapat memberikan bantuan saran terapi melalui berbagaipola histologi AdCC yang dinilai berdasarkan grading histopatologi.MetodePenelitian ini merupakan penelitian deskriptif analitik dengan pendekatan cross sectional. Sampel penelitian ini dari 35 blok parafindengan diagnosis adenoid cystic carcinoma yang ditentukan grading histopatologinya dan dipulas menggunakan CD117 dan EGFR.Penilaian dilihat berdasarkan intensitas pulasan dan persentase sel yang terwarnai pada membran dan/atau sitoplasma dandikelompokkan berdasarkan Allred scoring system.HasilCD117 terekspresi kuat pada AdCC 15/35 (42,9%) dan EGFR terekspresi kuat pada AdCC 21/35 (60%). Uji chi-square antaraekspresi CD117, EGFR dan grading histopatologi AdCC menunjukkan hubungan tidak bermakna, sedangkan uji McNemar antaraekspresi CD117, EGFR dan grading histopatologik AdCC menunjukkan hubungan bermakna p-value=0,031 (p<0,05).KesimpulanEkspresi CD117 dan EGFR memiliki proporsi yang sama pada AdCC kelenjar liur. CD117 dan EGFR dapat digunakan untukdeteksi AdCC kelenjar liur. Namun, ekspresi CD117 dan EGFR tidak berhubungan secara bermakna dengan grading histopatologiAdCC Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/402 10.55816/mpi.v29i1.402 Majalah Patologi Indonesia; Vol. 29 No. 1 (2020): MPI; 8 - 14 2527-9106 0215-7284 10.55816/mpi.v29i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/402/279

oai:ojs.pkp.sfu.ca:article/403 2025-12-06T00:58:32Z patologi:ART driver

Green Tea Extract Reduces Rat Lung Fibrotic Ekstrak Teh Hijau Mengurangi Luas Area Fibrosis Paru Tikus Desdiani Iris Rengganis Samsuridjal Djauzi Agus Setiyono Mohammad Sadikin Sri Widia A. Jusman Nurjati Chairani Siregar Suradi Putri C. Eyanoer green tea extract, lung fibrotic, Aschroft’s modified scale, fibropreventive, fibrolysis. ekstrak teh hijau, fibrosis paru, skor modifikasi Aschroft, fibropreventif, fibrolisis. BackgroundPulmonary fibrosis is often found in infectious diseases and chemical exposure in the work environment. Antifibrotic effects can befibropreventive and fibrolysis. Bleomycin can cause inflammation and pulmonary fibrosis. Catechin is proven can repair liverdamages due to alcohol induction. The aim of this study is to examine the effect of antifibrotic green tea catechin on reducing thearea of rat lung fibrotic.MethodsThis research was carried out in vivo experimentally in the Laboratory Animal Management Unit at Faculty of Veterinary MedicineIPB in 4 groups of rat experiments, consisting of 3 rats in the negative control group, 3 rats in the positive control group, 3 rats in thegroup of rats given bleomycin on the 8th day and 28thwith green tea extract every day from day 0 to 49 days (fibropreventive), 3groups of rats given bleomycin days 0 and 21 with green tea extract every day from day 15 to 49 (fibrolysis). Histopathologicalexamination was performe for all groups of rats.ResultsThis study showed that administration of green tea extract in fibropreventive groups and fibrolysis groups could reduce the fibrosisarea in rat lungs based on Aschroft's modified scale.ConclusionBased on the results of this study it was found that the anti-fibrotic activity of green tea extract was proven to be able to suppressthe development of pulmonary fibrosis both by fibropreventive and fibrolysis Latar belakangFibrosis paru sering ditemukan pada penyakit infeksi serta pajanan zat kimia di lingkungan kerja. Efek anti fibrotik dapat bersifatfibropreventif dan fibrolisis. Bleomisin dapat menyebabkan inflamasi dan fibrosis paru. Kandungan katekin terbukti mampumemperbaiki kerusakan pada hati akibat induksi alkohol. Tujuan penelitian ini untuk melihat efek anti fibrotik teh hijau yangmengandung senyawa katekin terhadap pengurangan luas area fibrosis paru tikus.MetodePenelitian ini dilakukan secara eksperimental in vivo acak di Unit Pengelolaan Hewan Laboratorium FKH IPB pada 4 kelompokhewan coba tikus, yang terdiri atas 3 ekor tikus kelompok kontrol negatif, 3 ekor kelompok kontrol positif, 3 ekor tikus kelompoktikus yang diberikan bleomisin hari ke-8 dan 28 serta ditambah ekstrak teh hijau setiap hari mulai hari ke-0 sampai ke-49 hari(fibropreventif), 3 ekor kelompok tikus yang diberikan bleomisin hari ke-0 dan 21 serta ditambah ekstrak teh hijau setiap hari mulaihari ke-15 sampai ke-49 (fibrolisis). Pemeriksaan histopatologi dilakukan terhadap semua kelompok tikus.HasilPenelitian ini menunjukkan bahwa pemberian ekstrak teh hijau pada kelompok fibropreventif dan kelompok fibrolisis, dapatmengurangi luas area fibrosis pada paru tikus berdasarkan skala modifikasi Aschroft.KesimpulanBerdasarkan hasil penelitian ini didapatkan bahwa aktivitas anti fibrotik ekstrak teh hijau terbukti mampu menekan perkembanganfibrosis paru baik secara fibropreventif maupun fibrolisis. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/403 10.55816/mpi.v29i1.403 Majalah Patologi Indonesia; Vol. 29 No. 1 (2020): MPI; 15 - 24 2527-9106 0215-7284 10.55816/mpi.v29i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/403/280

oai:ojs.pkp.sfu.ca:article/404 2025-12-06T00:58:32Z patologi:ART driver

Expression of p16 and CDK4 at Various Stages of T Laryngeal Carcinoma Ekspresi p16 dan CDK4 pada Berbagai Stadium T Karsinoma Laring Diana Purnamasari Dyah Fauziah CDK4, laryngeal carcinoma, p16. CDK4, karsinoma laring, p16. BackgroundDefect in cell cycle control affect cell proliferation and play an important role in pathogenesis of cancer. Recently, some proteins areknown to have an influence in cell cycle proliferation. P16 and CDK4 may have influenced in laryngeal carcinoma. CDK4, anoncogene, can form cyclin D1-CDK4 complex that inactivates pRb so that the cell cycle goes from G1 phase to S phase. P16, atumor suppressor protein, can prevent cyclin D1-CDK4 complex so that inhibit cell proliferation.MethodsThis research was an observational analysis study with paraffin block samples, consists of 3 samples of stage T1 and each 10samples of stage T2, T3 and T4 in Laboratory of Anatomical Pathology of RSUD dr. Soetomo that was collected from 2013-2015.Samples were stained with antibody of p16 and CDK4. P16 and CDK4 expression were assessed based on the percentage andintensity of tumor cells that were stained. The differences between variables were analyzed by Kruskal-Wallis. The correlationbetween variables was analyzed by Spearman correlation test.ResultsP16 expression of carcinoma larynx T1 (88.33±7.64), T2 (85.00±5.27), T3 (61.00±21.83), and T4 (62.00±25.30). Besides CDK4expression of carcinoma larynx T1 (43.33±15.28), T2 (47.00±14.18), T3 (75.00±8.50), and T4 (70.00±9.43). Statistic analysisshowed significant differences between p16 and CDK4 expression on each stade of carcinoma larynx (p=0.017 and p=0.000). Andthere were corelation between p16 and CDK4 expression on each stage of carcinoma larynx (p=0.000 and p=0.000).ConclusionThe lower p16 expression and the higher CDK4 expression showed the higher stage of carcinoma larynx. Latar belakangKegagalan kontrol siklus sel mempengaruhi proliferasi sel dan berperan penting dalam patogenesis kanker. Beberapa proteinmemiliki pengaruh pada siklus sel. Protein p16 dan CDK4 diduga mempunyai pengaruh pada karsinoma laring. P16, suatu tumorsuppressor protein, dapat mencegah terbentuknya kompleks cyclin D1-CDK4 sehingga menghambat proliferasi sel. CDK4, suatuonkogen, dapat membentuk kompleks cyclin D1-CDK4 dan menginaktifkan pRB sehingga siklus sel berjalan dari fase G1 ke fase Sdan meningkatkan proliferasi sel.MetodePenelitian ini merupakan studi analitik observasional yang dilakukan pada 3 sampel karsinoma laring stadium T1 dan masingmasing 10 sampel karsinoma laring stadium T2, T3 dan T4 di Laboratorium Patologi Anatomik RSUD dr. Soetomo yangdikumpulkan sejak tahun 2013-2015, dan dilakukan pulasan imunohistokimia dengan antibodi p16 dan CDK4. Ekspresi p16 danCDK4 dinilai berdasarkan prosentase sel tumor yang terpulas dan intensitasnya. Perbedaan ekspresi dianalisis dengan uji KruskalWallis. Korelasi antara variabel dianalisis dengan uji statistik Spearman.HasilEkspresi p16 pada karsinoma laring stadium T1 (88,33±7,64), T2 (85,00±5,27), T3 (61,00±21,83) dan T4 (62,00±25,30), sedangkanekspresi CDK4 pada karsinoma laring stadium T1 (43,33±15,28), T2 (47,00±14,18), T3 (75,00±8,50) dan T4 (70,00±9,43). Hasilanalisis statistik menunjukkan perbedaan antara ekspresi p16 dan CDK4 pada berbagai stadium karsinoma laring (p=0,017 danp=0,000) juga terdapat hubungan antara ekspresi p16 dan CDK4 pada berbagai stadium karsinoma laring (p=0,005 dan p=0,000).KesimpulanEkspresi p16 yang rendah dan ekspresi CDK4 yang tinggi menunjukkan stadium karsinoma laring yang tinggi. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/404 10.55816/mpi.v29i1.404 Majalah Patologi Indonesia; Vol. 29 No. 1 (2020): MPI; 25 - 29 2527-9106 0215-7284 10.55816/mpi.v29i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/404/281

oai:ojs.pkp.sfu.ca:article/405 2025-12-06T00:58:32Z patologi:ART driver

Tumor-infiltrating lymphocytes and their role in breast carcinoma Tumor-infiltrating lymphocytes dan Peranannya pada Karsinoma Payudara Ditha Diana Kusmardi breast carcinoma, tumor infiltrating lymphocytes karsinoma payudara, tumor infiltrating lymphocytes Infiltration of T lymphocyte cells in tumor tissue is a good prognosis in cancer patients. The infiltrating lymphocyte(TIL) tumor is the phenotype of CD4+ T lymphocytes and CD8+ T lymphocytes that infiltrate tumors. TIL can have abeneficial effect as well as a detrimental effect on breast carcinoma. There are three subtypes of T lymphocytes thatinfiltrate tumors which are T-helper (Th), T-citotoxic (Tc) and T-regulators (Treg). The role of Th lymphocytes in tumorimmunity can be divided into three phases, that are the initial induction phase, the phase maintaining the Tc responseas an effector and the memory formation phase. Th lymphocytes will secrete cytokines that can stimulate tumor cellsto increase the expression of MHC class I molecules, so that it will further optimize the function of Tc lymphocyticcytotoxicity which has the most dominant role in cytolytic tumor cells. Treg lymphocytes effectively suppressantitumor responses from Tc lymphocytes and other immune cells so that they can facilitate tumor development. Inbreast carcinoma, Treg lymphocytes have a different influence on each of the cancer subtypes. Infiltrasi sel limfosit T pada jaringan tumor merupakan prognosis yang baik pada penderita kanker. Tumour infiltratinglymphocyte (TIL) adalah fenotip dari limfosit T CD4+ dan limfosit T CD8+ yang menginfiltrasi tumor. TIL dapatmemberikan efek yang menguntungkan sekaligus juga efek yang merugikan pada karsinoma payudara. Terdapattiga subtipe limfosit T yang menginfiltrasi tumor yaitu limfosit T-helper (Th), T-citotoxic (Tc) dan T-regulator (Treg).Peranan limfosit Th dalam imunitas tumor dapat dibagi menjadi tiga fase, yaitu fase induksi awal, fasemempertahankan respon Tc sebagai efektor dan fase pembentukan memori. Limfosit Th akan mensekresikan sitokinyang dapat merangsang sel tumor untuk meningkatkan ekspresi molekul MHC kelas I, sehingga akan lebihmengoptimalkan fungsi sitotoksisitas limfosit Tc yang mempunyai peran paling dominan dalam sitolitik sel tumor.Limfosit Treg secara efektif mensupresi respon antitumor dari limfosit Tc dan sel imunitas lainnya sehingga dapatmempermudah perkembangan tumor. Pada karsinoma payudara, limfosit Treg mempunyai pengaruh yang berbedapada masing-masing subtipe kankernya. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/405 10.55816/mpi.v29i1.405 Majalah Patologi Indonesia; Vol. 29 No. 1 (2020): MPI; 30 - 40 2527-9106 0215-7284 10.55816/mpi.v29i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/405/282

oai:ojs.pkp.sfu.ca:article/406 2025-12-06T00:58:32Z patologi:ART driver

An Accuracy Comparison of SOX10 with DOG1 Immunohistochemistry in Diagnosing Acinic Cell Carcinoma of Salivary Gland in Paraffin Tissue Samples Perbandingan Akurasi Pemeriksaan Imunohistokimia SOX10 dengan DOG1 dalam Mendiagnosis Karsinoma Sel Asinik Kelenjar Liur pada Sediaan FFPE Ricky Handika Ika Dhuhani Nur Rahadiani Acinic cell carcinoma, Discovered on gist 1, DOG1, Salivary gland tumors, SOX10, Sry-related HMG box 10. Acinic cell carcinoma, Discovered on gist 1, DOG1, Salivary gland tumors, SOX10, Sry-related HMG box 10. BackgroundMorphological diversity is a challenge in establishing diagnosis of malignant tumor originated from salivary gland, especially Aciniccell carcinoma (ACC). Immunohistochemical (IHC) staining using DOG1 is often used to help diagnosis of ACC. Lately, IHC SOX10has been reported to have almost the same accuracy to DOG1 staining. This evidence-based case report aims to determine theaccuracy of SOX10 to DOG1 immunohistocheinstry staining in establishing a diagnosis of ACC.MethodsLiterature searches were carried out on the Pubmed, Clinical key, Proquest, ScienceDirect, Scopus, SpringerLink and Wileydatabases. Two journals were relevant to the clinical scenario, then a critical review is carried out using the Diagnostic StudyAppraisal Worksheet from the Center for Evidence-based Medicine, University of Oxford, 2010.ResultsThe accuracy of SOX10 in diagnosing ACC was slightly better than DOG1, with sensitivity reaching 100% and specificity reaching87.76%. The sensitivity of SOX10 and DOG1 is the same, but SOX10 is more specific, and positive predictive value is also betterthan DOG1.ConclusionThe accuracy of SOX10 is better than DOG1 in diagnosing ACC of salivary gland. However, the intensity of SOX10 staining tends tobe rather low compared to DOG1. Therefore, further research is still needed to recommend SOX10 staining in daily practice. Latar belakangKeanekaragaman morfologi menjadi tantangan tersendiri dalam menegakkan diagnosis tumor ganas kelenjar liur terutamaKarsinoma Sel Asinik (Acinic Cell Carcinoma/ACC). Pemeriksaan imunohistokimia (IHK) DOG1 banyak digunakan untuk membantumenegakkan diagnosis ACC. Belakangan ini SOX10 dilaporkan memiliki keakuratan yang hampir sama dibandingkan denganpulasan yang menggunakan antibodi DOG1. Laporan kasus berbasis bukti ini ditulis dengan tujuan mengetahui akurasi pulasan IHKSOX10 dibandingkan dengan pulasan IHK DOG1 dalam menegakkan diagnosis ACC kelenjar liur.MetodeDilakukan pencarian literatur pada basis data Pubmed, Clinical key, Proquest, ScienceDirect, Scopus, SpringerLink dan Wiley.Terdapat dua jurnal yang relevan dengan kasus, lalu dilakukan telaah kritis menggunakan Diagnostic Study Appraisal Worksheetdari Centre for Evidence-based Medicine, University of Oxford, 2010.HasilDari kedua jurnal, akurasi SOX10 dalam mendiagnosis acinic cell carcinoma kelenjar liur sedikit lebih baik dibandingkan denganDOG1, dengan sensitivitas mencapai 100% dan spesifitas mencapai 87,76%. Walaupun sensitivitas keduanya sama, namunSOX10 sedikit lebih spesifik, sehingga nilai positive predictive value nya juga lebih baik dibandingkan DOG1.KesimpulanAkurasi SOX10 lebih baik dibandingkan DOG1 dalam menegakkan ACC. Walau demikian, intensitas pulasanSOX10 cenderungrendah dibandingkan dengan DOG1 yang mayoritas intensitas pewarnaannya sedang dan kuat. Oleh karena itu, masih dibutuhkanpenelitian lebih lanjut untuk dapat mengeluarkan rekomendasi penggunaan antibody SOX1 di dalam pelayanan sehari-hari. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/406 10.55816/mpi.v29i1.406 Majalah Patologi Indonesia; Vol. 29 No. 1 (2020): MPI; 41 - 47 2527-9106 0215-7284 10.55816/mpi.v29i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/406/283

oai:ojs.pkp.sfu.ca:article/407 2025-12-06T00:58:32Z patologi:ART driver

Case Report and Literature Review: Giant Cell Tumor of Tendon Sheath Laporan Kasus dan Tinjauan Pustaka Giant Cell Tumor of Tendon Sheath Timotius Benedict Djitro Dyonesia Ary Haryanti Kidyarto Suryawinata Iwan Irawan Karman giant cell tumor of tendon sheath, histopathology, recurrence. giant cell tumor of tendon sheath, histopatologi, rekurensi BackgroundGiant cell tumor of tendon sheath (GCTTS) is the 2nd most common tumor in the hand but is relatively rare ineveryday practice. The tumor has a tendency to develop in women aged 30-50 years and has no specific symptoms.This characteristic made GCTTS difficult to distinguish clinically from other tumor occurring mainly in the hand. Inaddition, high recurrence rate also made these tumors often reappear despite having been excised. As such,preoperative histopathologic examination is important in enforcing the diagnosis so that appropriate steps can betaken to suppress the recurrence rate of this tumor Latar belakangGiant cell tumor of tendon sheath (GCTTS) adalah tumor tersering kedua pada tangan, tetapi relatif jarang ditemuipada praktek sehari-hari. Tumor dengan kecenderungan timbul pada wanita berusia 30-50 tahun ini tidak memilikigejala yang spesifik sehingga membuat tumor ini sulit dibedakan secara klinis dari tumor lain yang juga memilikipredileksi pada tangan. Selain itu, angka rekurensi yang tinggi juga membuat tumor ini sering muncul kembalisekalipun telah dilakukan eksisi. Pemeriksaan histopatologi preoperatif penting dalam penegakan diagnosis sehinggapenanganan yang tepat dapat diambil untuk menekan rekurensi Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/407 10.55816/mpi.v29i1.407 Majalah Patologi Indonesia; Vol. 29 No. 1 (2020): MPI; 48 - 51 2527-9106 0215-7284 10.55816/mpi.v29i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/407/284

oai:ojs.pkp.sfu.ca:article/408 2025-12-06T00:58:32Z patologi:ART driver

The Effect of Myrmecodia pendans Ethanol Extract on Blood Vessels Within Pulpitis (Study on Sprague Dawley Rats) Janti Sudiono Patricia Revana Key words: Myrmecodia pendans, pulpitis, blood vessel appearance, anti inflammation Sarang semut (Myrmecodia pendans) is one of traditional herbs used to treat various diseases. Myrmecodia pendanscontain chemical compound flavonoid which has anti inflammation effects. Pulpitis is a dental pulp inflammation thatcan be caused by bacteria invasion, physical trauma or iatrogenic factor which affecting blood vessels in dental pulp.This research is an in vivo laboratory experimental pre and post treatment with control. The purpose of this study is tofind out the effects of Myrmecodia pendans ethanol extract on blood vessels appearance of Sprague Dawley rats’dental pulps which had been inducted with 0,01ml Porphyromonas gingivalis for 48 hours. The subjects were dividedinto 3 groups with Group I representing the negative control, group II representing treated group with Myrmecodiapendans ethanol extract, and group III representing positive control with Ca(OH)2. Group II and III were divided intosubgroups based on the treatment periods of 48, 96, 168, 336 hours. The statistic results showed that there weresignificant differences (p<0.05) among the pulpitis, negative control, positive control Ca(OH)2 and the Myrmecodiapendans treatment subgroups of 48, 96, 168, 336 hours. Myrmecodia pendans ethanol extract has comparable antiinflammation effect with Ca(OH)2 which is used to pulpitis commercial medicament. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/408 10.55816/mpi.v29i1.408 Majalah Patologi Indonesia; Vol. 29 No. 1 (2020): MPI; 52 - 56 2527-9106 0215-7284 10.55816/mpi.v29i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/408/285

oai:ojs.pkp.sfu.ca:article/409 2022-05-21T13:32:47Z patologi:ART driver

Expression of HPV16 Oncoprotein E7 in Precancerous Lesions and Cervical Squamous Cell Carcinoma Ekspresi HPV16 Onkoprotein E7 pada Lesi Prakanker dan Karsinoma Sel Skuamosa Serviks Saniba, Verli Wresnindyatsih Musa, Zulkarnain Hafy, Zen HPV16 E7, precancerous lesions, SCC. lesi prakanker, KSS, HPV16 E7 BackgroundCervical cancer is the secondmost common malignancy in women worldwide.The vast majority of cervical cancersare associatedwith high risk HPV16 infection. The persistence of HPV16 and integration of viral DNA into cell genome are necessary for thedevelopment of precancerous lesion and squamous cell carcinoma (SCC) of the cervix. The integration of viral DNA in the host cellgenome resulted in the regulation of oncoprotein E7 leading to detection of HPV16 E7 expression in precancerous lesions and SCCof the cervix.The aim of this study was to evaluate differences of HPV16 E7 expression in precancerous lesions and cervical SCC.MethodsAn observational study with cross-sectional design was performedat Department of Anatomical Pathology Faculty of MedicineUniversity of Sriwijaya/dr. Mohammad Hoesin General Hospital Palembang, from 1st July 2014 to 30st Juni 2017. The selectedsamples consisted of 25 precancerous cases lesions (13 cases of LSIL and 12 samples of HSIL) and SCC (25 cases).Immuhistochemical stained was conducted using HPV16 E7 antibody. Fisher exact’s test was used to analyze differences inexpression of HPV16 E7 among precancerous lesions and cervical SCC.ResultsThe pracancerous lesions occurred mostly between the ages of 30-40 years, while SCC were those between the ages of 51-60years. All SCC cases (100%) and 91.6% of HSIL showed weak to strong HPV16 E7 expression, and 84.6% cases of LSIL showedimmunonegative.ConclusionThe positivity of HPV16 E7 increased in HSIL and KSS lesions. Latar belakangKanker serviks merupakan keganasan kedua terbanyak pada wanita di dunia, yang sebagian besar disebabkan oleh infeksi humanpapillomavirus(HPV) risiko tinggi yaitu HPV tipe 16. Persistensi infeksi HPV16 dan integrasi DNA virus pada genom sel pejamuberperan terhadap perkembangan lesi prakanker dan KSS serviks. Integrasi DNA virus pada genom sel pejamu mengakibatkanregulasi onkoprotein E7. Ekspsresi HPV16 E7 dapat dideteksi pada lesi prakanker dan KSS serviks. Penelitian ini bertujuan untukmengetahui perbedaan ekspresi HPV16 E7 pada lesi prakanker dan KSS serviks.MetodePenelitian retrospektif observasional dengan dengan pendekatan cross-sectional. Populasi penelitian adalah blok parafin sediaanlesi prakanker (LSIL, HSIL) dan KSS serviks yang tersimpan di Departemen Patologi Anatomik RSUP Dr. Moh. Hoesin Palembangperiode 1 Juli 2014 sampai dengan 30 Juni 2017. Jumlah sampel 50 kasus, dari masing-masing kelompok kasus dipilih: lesiprakanker (25 kasus, meliputi 13 kasus LSIL dan 12 kasus HSIL) dan KSS (25 kasus). Slaid blok parafin yang memenuhi kriteriadilakukan pulasan imunohistokimia menggunakan antibodi HPV16 E7. Perbedaan ekspresi HPV16 E7 pada lesi prakanker dan KSSserviks dianalisis menggunakan uji Fisher Exact’s.HasilUsia terbanyak pasien lesi prakanker adalah 30-40 tahun dan KSS adalah 51-60 tahun. Ekspresi HPV16 E7 positif lemah sampaikuat pada 100% kasus KSS dan 91,6% kasus HSIL, sedangkan ekspresi HPV16 E7 negatif pada 86,4% kasus LSIL. Analisisstatistik ekspresi HPV16 E7 antara lesi prakanker dan KSS serviks menunjukkan perbedaan bermakna (p=0,000).KesimpulanPositivitas ekspresi HPV16 E7 meningkat pada lesi HSIL dan KSS. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/409 10.55816/mpi.v29i2.409 Majalah Patologi Indonesia; Vol. 29 No. 2 (2020): MPI; 57 - 64 2527-9106 0215-7284 10.55816/mpi.v29i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/409/286

oai:ojs.pkp.sfu.ca:article/410 2022-05-21T13:33:26Z patologi:ART driver

Identification of CYP1A1 Gene Polymorphism in Squamous Cell Carcinoma and Cervical Adenocarcinoma Identifikasi Polimorfisme Gen CYP1A1 pada Karsinoma Sel Skuamosa dan Adenokarsinoma Serviks Fitriyani, Hilda Delyuzar Hidayat polymorphism, CYP1A1, 3801TC, Ile462Val, PCR-RFLP CYP1A1, PCR-RFLP, polimorfisme, 3801TC, Ile462Val. BackgroundCervical cancer is the third most common cancer in women with risk factor of smoking, high parity, long term use of oralcontaception that are associated with chemical carcinogenesis. Chemical carcinogenesis require biotransfor-mation of lipophilicsubstrates to hydrophilic metabolites, therefore facilitating their secretion from the human body. Cytochrome P450 (CYP) is one ofgenes that have important role in this process. Benzo[α]pyrene and estrogen have a common biotransformation process which ismetabolized by CYP, particularly CYP1A1. The objectives to identify the frequency and distribution of CYP1A1 gene polymorphismin squamous cell carcinoma and adenocarcinoma of the cervix.MethodsThis is an analytical descriptive study with cross sectional approach. CYP1A1 gene polymorphism (3801T/C or Ile462Val) wasanalyzed using PCR-RFLP method followed by gel electrophoresis.ResultsCYP1A1 gene polymorphisms (3801TC) in squamous cell carcinoma were 50% heterozygote T/C, 36% wild-types T/T and 14%homozygote C/C. CYP1A1 gene polymorphisms (3801TC) in adenocarcinoma were 60% heterozygote T/C and 40% wild-types T/T.CYP1A1 gene polymorphisms (Ile462Val) in squamous cell carcinoma were 97.2% heterozygote Ile/Val, and 2.8% homozygoteVal/Val. CYP1A1 gene polymorphisms (Ile462Val) in adenocarcinoma were 100% heterozygote Ile/ValConclusionThe most common type of CYP1A1 gene polymorphism (3801TC and Ile462Val) in squamous cell carcinoma and adenocarcinomaof the cervix were heterozygote. Latar belakangKanker serviks merupakan kanker ketiga tersering pada wanita dengan faktor risiko merokok, paritas tinggi dan lamanyapenggunaan kontrasepsi oral yang berkaitan dengan karsinogen kimiawi dan memicu terjadinya proses karsinogenesis. Karsinogenkimiawi memerlukan biotransformasi dari lipofilik menjadi hidrofilik, agar lebih mudah untuk diekskresikan dari dalam tubuh. Salahsatu gen yang berperan penting dalam proses biotransformasi tersebut adalah cytochrome P450 (CYP). Benzo[a]pyrene danestrogen memiliki kesamaan dalam proses biotransformasi, dimetabolisme oleh CYP, khususnya CYP1A1. Tujuan penelitian iniuntuk mengidentifikasi polimorfisme gen CYP1A1 pada karsinoma sel skuamosa dan adenokarsinoma serviks.MetodePenelitian ini merupakan penelitian deskriptif dengan pendekatan cross sectional. Polimorfisme gen CYP1A1 (3801T/C danIle462Val) dianalisis dengan menggunakan metode PCR-RFLP dan dilanjutkan dengan gel elektroforesis.HasilPolimorfisme gen CYP1A1 (3801TC) ditemukan pada karsinoma sel skuamosa dengan genotip heterozygot T/C 50%, wild-typesT/T 36% dan homozygot C/C 14%. Polimorfisme gen CYP1A1 (3801TC) pada adenokarsinoma dengan genotip heterozygot T/C60% dan wild-types T/T 40%. Polimorfisme gen CYP1A1 (Ile462Val) pada karsinoma sel skuamosa dengan genotip heterozygotIle/Val 97,2% dan homozygot Val/Val 2,8%. Polimorfisme gen CYP1A1 (Ile462Val) pada adenokarsinoma dengan genotipheterozygot Ile/Val 100%KesimpulanPolimorfisme gen CYP1A1 (3801TC dan Ile462Val) pada karsinoma sel skuamosa dan adenokarsinoma serviks yang tertinggiadalah tipe heterozygot Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/410 10.55816/mpi.v29i2.410 Majalah Patologi Indonesia; Vol. 29 No. 2 (2020): MPI; 65 - 70 2527-9106 0215-7284 10.55816/mpi.v29i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/410/287

oai:ojs.pkp.sfu.ca:article/415 2022-05-21T13:34:00Z patologi:ART driver

Role of CD44 in Non-Alcoholic Steatohepatitis (NASH) Progressivity Peran CD44 pada Progresivitas Non Alcoholic Steatohepatitis (NASH) Sarwanti Stephanie, Marini Kodariah, Ria NAFLD, steatohepatitis, CD44. NAFLD, steatohepatitis, CD44 Non alcoholic Steatohepatitis (NASH) is part of a group of conditions called Non-Alcoholic Fatty Liver Diseases(NAFLD) where it is a chronic disease, which defined after elimination other causes of fatty liver, such as excessivealcohol consumption and other causes of chronic liver diseases. NASH is fatty liver disease which characterized byballooning of hepatocyte and lobular inflammation with or without fibrosis. Histopathology diagnose on NASH can bedefined by performing liver biopsy. The purpose of liver biopsy is to define level and degree of the disease. Cluster ofDifferentiation (CD) 44 is a transmembrane glycoprotein receptor which located on the surface of macrophage cells,lymphocytes and endothelial cells. In studies which conducted on mice and humans, showed that CD44 playsimportant role in the progression of NASH. CD44 regulates inflammation of adipose tissue and liver. CD44 ispresumed as a marker which increase macrophage infiltration and other inflammatory cells on liver. This processleads to ultimate increment on insulin resistance and fatty liver. Deficiency was discovered on mice which injectedwith methionine and choline deficiency diet (MCDD). CD44 is associated with preventive method to prevent livercomplication by reducing macrophage or monocyte and as well as neutrophil accumulation in liver which wasevaluated through reducing numbers of inflammatory focus, expression of inflammatory cytokines: tumor necrosisfactor α (TNFα), interleukin (IL) -1B and nitric oxide synthesis (iNOS), and pro-inflammatory types of macrophage. Inobese patients, number of CD44 is predicted to be increasing. ABSTRAKNon alcoholic Steatohepatitis (NASH) merupakan suatu spektrum penyakit dari Non Alcoholic Fatty Liver Disease(NAFLD) yang bersifat kronik. Setelah menyingkirkan penyebab perlemakan hati lainnya seperti konsumsi alkoholberlebih dan penyakit hati kronik lainnya. NASH ditandai dengan adanya perlemakan hati yang disertai peradangan(balloning) hepatosit dan inflamasi lobular dengan atau tanpa disertai adanya fibrosis. Diagnosis histopatologi NASHhanya dapat dilakukan dengan melakukan biopsi hati agar didapatkan tingkat dan derajat penyakitnya. Cluster ofDifferen-tiation (CD) 44 merupakan reseptor glikoprotein transmembran yang terletak dipermukaan sel makrofag,limfosit, sel endotel. Pada penelitian yang dilakukan pada mencit dan manusia didapatkan hasil bahwa CD44berperan penting pada progresivitas NASH melalui mekanisme peningkatan peradangan pada jaringan adiposa danhati. CD44 diduga meningkatkan rekruitmen makrofag dan sel-sel radang lainnya pada jaringan hati yang padaakhirnya meningkatkan resistensi insulin dan perlemakan pada hati. Pemberian methionine and choline deficiencydiet (MCDD) pada model tikus didapatkan defisiensi CD44 dan berhubungan dengan pencegahan komplikasi hatikarena berkurangnya infiltrasi dan inflamasi monosit atau makrofag dan rekrutmen neutrofil di hati yang dievaluasimelalui berkurangnya jumlah fokus inflamasi, ekspresi sitokin peradangan seperti tumor necrosis factor α (TNFα),interleukin (IL) -1B dan nitric oxide synthesis (iNOS) serta makrofag tipe pro inflamasi. CD44 juga diduga meningkatekspresinya secara spesifik pasien obesitas. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/415 10.55816/mpi.v29i2.415 Majalah Patologi Indonesia; Vol. 29 No. 2 (2020): MPI; 71 - 81 2527-9106 0215-7284 10.55816/mpi.v29i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/415/290

oai:ojs.pkp.sfu.ca:article/417 2022-05-21T13:34:53Z patologi:ART driver

Relationship of CD-68 Associated Macrophages Tumor Density and Clinicopathological Characteristics in Invasive Breast Carcinoma Hubungan Kepadatan Tumor Associated Macrophages CD-68 dan Karakteristik Klinikopatologi pada Karsinoma Payudara Invasif Handayani, Miko Kresna Maulani, Heni Apriyani, Nursanti invasive carcinoma of the breast, molecular subtype, TAM, CD-68. karsinoma payudara invasif, subtipe molekuler, TAM, CD68 BackgroundBreast carcinoma is the most common malignancy among women worldwide. Many factors can influence prognosis of the disease.Tumor microenvironment (TME) is enriched in highly active immune cells. Herein, tumor associated macrophages (TAM) are themost prevalent immune cells. TAM can enhance tumor progression by promoting angiogenesis, invasion and metastasis. Somestudies demonstrate that high density of macrophages infiltration associated with poor prognosis. The aim of the study is todetermine the association between TAM density and clinicopathological characteristics of breast invasive carcinoma.MethodsDescriptive study with case series design on January 1, 2015-October 31, 2017 was performed at Department of AnatomicalPathology Faculty of Medicine University of Sriwijaya/dr. Moh. Hoesin General Hospital Palembang on 48 samples invasivecarcinoma mammae subtype unspecific, and have already staining with immunohistochemistry staining ER, PR, HER-2 and Ki-67,surrogate markers. Then immunohistochemistry staining using CD-68 antibody was conduced. The association between TAMdensity and clinicopathological characteristics were analyzed by using Spearman’s Rho test.ResultsThe majority of the patient are older than 40 years of age (77.1%), with positive lymphovascular invasion (79.2%), and at the gradeIII (62.5%). Bivariate analysis between TAM CD-68+density in stromal tumor and subtype luminal A showed negative correlationmoderate significantly (r= -0.398; p=0.005) while between TAM CD-68+density in stromal tumor and subtype triple negative showedpositive correlation moderate significantly (r=0.335; p=0.020).ConclusionTAM CD-68+density in stromal tumor was significantly correlated with luminal A and triple negative subtype. ABSTRAKLatar belakangKarsinoma payudara merupakan keganasan terbanyak pada wanita. Banyak faktor yang mempengaruhi prognosis penyakit ini.Tumor microenvironment (TME) atau ekologi tumor terutama terdiri dari sel-sel imun dengan tumor associated macrophages (TAM)sebagai populasi utama, yang berperan pada proses angiogenesis, invasi dan metastasis karsinoma. Beberapa penelitian telahmemperlihatkan bahwa infiltrasi tinggi TAM dikaitkan dengan prognosis yang buruk. Penelitian ini bertujuan untuk mengetahuihubungan antara kepadatan TAM dengan karakteristik klinikopatologi karsinoma payudara invasif.MetodePenelitian deskriptif dengan desain serial kasus, mulai 1 Januari 2015`-31 Oktober 2017 telah dilakukan di Departemen PatologiAnatomik Fakultas Kedokteran Universitas Sriwijaya/Rumah Sakit Umum dr. Mohamad Hoesin Palembang pada 48 sampel yangtelah didiagnosis secara histopatologik sebagai karsinoma payudara invasif tipe tidak spesifik dan telah dilakukan pemeriksaanpulasan imunohistokimia ER, PR, HER-2 dan Ki67. Kemudian dilakukan pewarnaan imunohistokimia dengan menggunakanantibodi CD-68. Hubungan antara kepadatan TAM dengan karakteristik klinikopatologi dianalisa dengan uji Spearman’s Rho.HasilPada penelitian ini didapatkan mayoritas pasien berusia >40 tahun (77,1%), invasi limfovaskular positif (79,2%), dan grade III(62,5%). Analisis bivariat antara kepadatan TAM CD-68 stromal dan subtipe luminal A menunjukkan korelasi negatif sedangbermakna (r= -0,398; p= 0,005), sedangkan antara kepadatan TAM CD-68 stromal dan subtipe triple negative menunjukkan korelasipositif sedang bermakna (r=0,335; p=0,020).KesimpulanKepadatan TAM CD-68 stromal tumor berhubungan erat dengan subtipe luminal A dan triple negative Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/417 10.55816/mpi.v29i2.417 Majalah Patologi Indonesia; Vol. 29 No. 2 (2020): MPI; 82 - 88 2527-9106 0215-7284 10.55816/mpi.v29i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/417/291

oai:ojs.pkp.sfu.ca:article/418 2022-05-21T13:35:25Z patologi:ART driver

Analysis of Immunoexpression of Programmed Death Ligand 1 (PD-L1) and Proliferation Index Ki-67 on Diffuse Astrocytic Tumours (DAT) Analisis Imunoekspresi Programmed Death Ligand 1 (PD-L1) dan Indeks Proliferasi Ki-67 pada Tumor Astrositik Difus Lubis, Billy Parulian Agustina, Hasrayanti Usman, Hermin Aminah Hernowo, Bethy Surjawathy Sri Suryanti Faried, Ahmad Bolly, Hendrikus histopathological degree, Ki-67, PD-L1, diffuse astrocytic tumours. derajat histopatologi, Ki-67, PD-L1, tumor astrositik difus BackgroundDiffuse astrocytic tumours (DAT) is a diffuse infiltrative astrocytoma tumor accompanied by molecular parameters in the form or absence ofIsocitrate Dehydrogenase (IDH) gene mutations. The DAT category without the examination of the IDH gene mutation is classified as NotOtherwise Specified (NOS). Ki-67 is a marker for DAT proliferation so that it can assess the degree of DAT. Diffuse astrocytic tumoursbehavior is influenced by proliferative ability and the presence of a tumor microenvironment. The tumor microenvironment PD-L1 factorinfluences DAT behavior and allows the development of target therapy in DAT cases. This study aimed to analyze the relationship betweenPD-L1 and Ki-67 imunoexpression in the histopathological degree of DAT.MethodsCross-sectional study of 30 paraffin blocks of DAT NOS cases from 2014-2018 in the Department of Anatomical Pathology RSUP dr. HasanSadikin, Bandung. The sample consisted of 15 cases of grade II, 7 cases of grade III and 8 cases of grade IV, carried outimmunohistochemical staining with GFAP, PD-L1 and Ki-67. Assessment of PD-L1 immunoexpression was carried out by the histoscoremethod. The Ki-67 assessment was assessed based on its distribution, which was <10% and ≥10%. PD-L1 and Ki-67 immunoexpressionwith histopathological degrees in DAT were statistically analyzed by Kolmogorof Smirnov test, however association betweenimmunoexpression of PD-L1 and Ki-67 were analyzed by chi-square test.ResultsThere was a significant relationship between PD-L1 immunoexpression and histopathological degree in DAT (p=0.005). There was asignificant relationship between Ki-67 immunoexpression and DAT histopathology degree and with PD-L1 (p=0.001 and p=0.010).ConclusionPD-L1 immunoexpression and proliferation rates affect the degree of DAT. Proliferation rate of DAT is influenced by PD-L1 so that in thisstudy, PD-L1 and Ki-67 can be used as a marker of prognosis predictors. Latar belakangTumor astrositik difus (TAD) merupakan tumor astrositoma difus yang infiltratif disertai dengan parameter molekular berupa ada atautidaknya mutasi gen Isositrat Dehidrogenase (IDH). Kategori TAD tanpa disertai pemeriksaan mutasi gen IDH diklasifikasikan sebagai NotOtherwise Specified (NOS). Ki-67 merupakan penanda untuk melihat proliferasi TAD sehingga dapat menilai derajat TAD. Perilaku TADdipengaruhi oleh kemampuan proliferasi serta adanya tumor microenvironment. Faktor tumor microenvironment PD-L1 mempengaruhiperilaku TAD dan memungkinkan pengembangan terapi target pada kasus TAD. Penelitian ini bertujuan untuk menganalisis hubunganantara imunoekspresi PD-L1 dan Ki-67 pada derajat histopatologi TAD.MetodePenelitian potong lintang pada 30 blok parafin kasus TAD NOS sejak tahun 2014-2018 di Departemen Patologi Anatomik RSUP dr. HasanSadikin, Bandung. Sampel terdiri atas 15 kasus derajat II, 7 kasus derajat III, dan 8 kasus derajat IV yang dilakukan pewarnaanimunohistokimia dengan GFAP, PD-L1 dan Ki-67. Penilaian imunoekpresi PD-L1 dilakukan dengan metode histoscore. Penilaian Ki-67dinilai berdasarkan distribusinya yaitu <10% dan ≥10%. Imunoekspresi PD-L1 dan Ki-67 dengan derajat histopatologi pada TAD dianalisissecara statistik dengan uji Kolmogorov Smirnov, sedangkan hubungan imunoekspresi PD-L1 dan imunoekspresi Ki-67 dinilai dengan uji chisquare.HasilTerdapat hubungan yang signifikan antara imunoekpresi PD-L1 dengan derajat histopatologi pada TAD (p=0.005). Terdapat hubungan yangsignifikan antara imunoekpresi Ki-67 dengan derajat histopatologi TAD dan dengan PD-L1 (p=0,001 dan p=0,010).KesimpulanImunoekspresi PD-L1 dan tingkat proliferasi mempengaruhi derajat TAD. Tingkat proliferasi TAD dipengaruhi oleh PD-L1 sehingga padapenelitian ini, PD-L1 dan Ki-67 dapat digunakan sebagai salah satu penanda prediktor prognosis. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/418 10.55816/mpi.v29i2.418 Majalah Patologi Indonesia; Vol. 29 No. 2 (2020): MPI; 89 - 94 2527-9106 0215-7284 10.55816/mpi.v29i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/418/293

oai:ojs.pkp.sfu.ca:article/419 2022-05-21T13:36:04Z patologi:ART driver

Analysis Morphological of Tuberculosis Lymphadenitis Features between Cytology Biopsy of Fine Needle Aspiration Polymerase Chain Reaction Methods Analisis Gambaran Morfologi Limfadenitis Tuberkulosis Menggunakan Metode Biopsi Aspirasi Jarum Halus dan Polymerase Chain Reaction Suryadi, Dedy Delyuzar Soekimin TB, lymphadenitis, aspiration biopsy, morphology, PCR. TB, limfadenitis, biopsi aspirasi, morfologi, PCR. BackgroundIndonesia is the 2nd largest of tuberculous (TB) cases in the world. Therefore, TB must be controlled by increasing the capacity anddeveloping universal access needed for TB diagnose accurately and quickly. Progression in controlling TB and decreasing theconsequence can be accelerated through early detection and promt treatment. This study used PCR test as gold standard, albeitthe high cost. Thus, quick, accurate and low cost diagnostic method is needed. In this case morphologic features requirementinclude the existence of epithelioid and necrosis in FNAB smears. Objective was to analyze morphologic features of tuberculosislymphadenitis using FNAB and PCR methods.MethodsThis study is a cross sectional analysis. N samples are all kind of lymphadenitis tuberculosis. Morphologic features in FNABcytology must be confirmed using PCR test and performing chi-square test respectively.ResultsEpitheloid and necrosis features of TB lymphadenitis on FNAB showed 100% positive on PCR methods. This study divided intothree groups. Statistic analyses between group I and II, group II and III showed in significant differences (p=0.074); while betweengroup I and III showed significant differences (p<0.05).ConclusionsPCR method could confirmed morphologic features (epitheloid and necrosis) of TB lymphadenitis Latar belakangIndonesia termasuk negara kedua dengan kasus TB (tuberkulosis) terbanyak di dunia, sehingga TB di Indonesia harus dikendalikandengan cara meningkatkan kapasitas dan membangun akses universal yang dibutuhkan untuk menegakkan diagnosis laboratoriumyang cepat dan akurat. Kemajuan dalam mengontrol TB dan mengurangi konsekuensinya dapat dipercepat melalui diagnosis dinidan pengobatan yang tepat. Penelitian ini menggunakan pemeriksaan polymerase chain reaction (PCR) sebagai baku emas,walaupun untuk diagnosis rutin membutuhkan biaya yang lebih mahal. Oleh karena itu sangat dibutuhkan metode diagnostik yangcepat, akurat dan tidak mahal yaitu biopsi aspirasi jarum halus (BAJAH) dengan gambaran morfologi berupa epiteloid dan nekrosis.Tujuan penelitian ini untuk menganalisis gambaran morfologi limfadenitis TB menggunakan BAJAH dan PCR.MetodePenelitian ini merupakan studi analitik dengan pendekatan potong lintang. Jumlah sampel adalah n sampel, yaitu semua pasienlimfadenitis tuberkulosis. Kriteria gambaran morfologi yang berupa epiteloid dan nekrosis pada pemeriksaan sitologi BAJAH danPCR, kemudian dilakukan uji chi-square.HasilGambaran morfologi limfadenitis TB yang berupa epiteloid dan nekrosis pada pemeriksaan sitologi BAJAH menunjukkan hasilpemeriksaan PCR 100% positif. Analisis statistik menggunakan Fisher exact antara grup I dan grup II, grup II dan grup IIImenunjukkan perbedaan tidak bermakna (p=0,074); sedangkan antara grup I dan grup III menunjukkan perbedaan bermakna(p<0,05).KesimpulanMetode PCR dapat digunakan untuk mengkonfirmasi gambaran morfologik (epiteloid dan nekrosis) lymfadenitis TB. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/419 10.55816/mpi.v29i2.419 Majalah Patologi Indonesia; Vol. 29 No. 2 (2020): MPI; 95 - 100 2527-9106 0215-7284 10.55816/mpi.v29i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/419/295

oai:ojs.pkp.sfu.ca:article/420 2022-05-21T13:36:33Z patologi:ART driver

Difference in CD56 Expression in Nodular Hyperplasia, Follicular Adenomas and Thyroid Papillary Carcinoma Perbedaan Ekspresi CD56 pada Hiperplasia Nodular, Adenoma Folikular dan Karsinoma Papiler Tiroid Octaviani Dewi, Amalia Unita, Mezfi Apitriani nodular hyperplasia, follicular adenoma, papillary carcinoma, CD56 hiperplasia nodular, adenoma folikular, karsinoma papiler, CD56 BackgroundThyroid carcinomas are the most common malignancy of endocrine organs and the fourth most common cancer of women inIndonesia. Most of these tumors derived from thyroid follicular cells and more than 85% of cases are papillary carcinoma. Somecases may be confusing and cause misdiagnosis due to the morphology of these tumors are frequent overlap between nodularhyperplasia, follicular adenoma and papillary carcinoma. Anti-CD56 is one of the diagnostic markers with high sensitivity andspecificity in distinguishing benign thyroid lesions and papillary carcinoma. The aims of this study is to analyze the differences ofCD56 expression in nodular hyperplasia, follicular adenoma and papillary carcinoma.MethodsAn observational study with cross-sectional design was performed at Departement of Anatomical Pathology Faculty of MedicineUniversity of Sriwijaya/Dr. Mohammad Hoesin Palembang, from 1st January 2014 to 30th June 2017. Paraffin blocks of 52 samplesare collected from nodular hyperplasia (24 cases), follicular adenoma (8 cases) and papillary carcinoma (20 cases). The slides wereimmune stained using CD56 antibody. The difference of CD56 expression between papillary carcinoma and nodular hyperplasiawas analyzed using Chi-Square test while those between papillary carcinoma among follicular adenoma was analyzed usingFisher’s exact test.ResultsIn all samples of papillary carcinoma CD56 expression was negative compared to totally positive reaction in all samples of nodularhyperplasia and follicular adenoma.ConclusionAnti-CD56 could be used as a diagnostic marker in distinguishing between papillary carcinoma, nodular hyperplasia and follicularadenoma. Latar belakangKarsinoma tiroid merupakan keganasan tersering organ endokrin dan menempati urutan keempat tumor terbanyak padaperempuan di Indonesia. Sebagian besar tumor ganas ini berasal dari diferensiasi sel folikular tiroid dan lebih dari 85% merupakankarsinoma papiler. Beberapa kasus dapat membingungkan sehingga menimbulkan kesulitan diagnosis dikarenakan secaramorfologi mempunyai gambaran tumpang tindih antara hiperplasia nodular, adenoma folikular dengan karsinoma papiler. CD56merupakan salah satu petanda diagnostik yang memiliki sensitivitas dan spesifisitas tinggi dalam membedakan lesi-lesi tiroidtersebut dengan menunjukkan penurunan ekspresi CD56 pada karsinoma papiler. Tujuan penelitian ini menganalisis perbedaanekspresi CD56 antara hiperplasia nodular, adenoma folikular dan karsinoma papiler tiroid.MetodePenelitian bersifat observational dengan pendekatan cross-sectional di Departemen Patologi Anatomik RSUP Dr. MohammadHoesin Palembang periode 1 Januari 2014 sampai 30 Juni 2017. Sampel blok parafin sebanyak 52 sampel (24 kasus hiperplasianodular, 8 kasus adenoma folikular dan 20 kasus karsinoma papiler), dari masing-masing kelompok kasus dipilih, kemudiandilakukan pulasan imunohistokimia menggunakan antibodi anti CD56. Perbedaan ekspresi CD56 pada karsinoma papiler danhiperplasia nodular dengan uji chi-square sedangkan karsinoma papiler dan adenoma folikular dengan uji Fisher’s exact.HasilKarsinoma papiler menunjukkan ekspresi CD56 negatif, sedangkan hiperplasia nodular dan adenoma folikular menunjukkanekspresi CD56 positif.KesimpulanCD56 dapat digunakan sebagai petanda diagnostik untuk membedakan antara karsinoma papiler, hiperplasia nodular dan adenomafolikular, dan berguna meningkatkan akurasi diagnosis. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/420 10.55816/mpi.v29i2.420 Majalah Patologi Indonesia; Vol. 29 No. 2 (2020): MPI; 101 - 106 2527-9106 0215-7284 10.55816/mpi.v29i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/420/296

oai:ojs.pkp.sfu.ca:article/421 2022-05-21T13:37:45Z patologi:ART driver

Analysis of Cyclin D1 and COX-2 Expressions in Various Histopathological Grading of Ovary Endometrioid Carcinoma Analisis Ekspresi Cyclin D1 dan COX-2 pada Berbagai Grading Histopatologik Karsinoma Endometrioid Ovarium Sunardi, Erwin Joewarini, Endang COX-2, Cyclin D1, endometrioid carcinoma. COX-2, Cyclin D1, endometrioid carcinoma, ovarium. BackgroundOvarian endometrioid carcinoma accounts for 10-15% of ovarian carcinomas, representing the second most common form ofovarian epithelial malignancy. Epithelial cell lines stably transfected to overexpress COX-2 exhibit a higher proliferation rate and aninhibition of apoptosis by prolongation of the G1 phase of the cell cycle through effects on Cyclin D1. The aim of this study is toanalyzed the correlation and differences between Cyclin D1 and COX-2 expressions in various histopathology grading ovarianendometrioid carcinoma.MethodsAn analitycal obsevational design with cross-sectional approach on sample from 23 paraffin blocks in Anatomical PathologyLaboratory Dr. Soetomo General Hospital. Microscopic slide stained with Cyclin D1 and COX-2 antibody. The imunohistochemistryexpression are evaluated and analyzed using Kruskal Wallis and Spearman test.ResultsThere were no differences Cyclin D1 expression in each grade, there were no differences COX-2 expression in each grade, therewas no correlation between Cyclin D1 and COX-2 expression all histopathologic grading, but have correlation expression in grade 3ovarian endometrioid carcinoma.ConclusionThis study showed that Cyclin D1 and COX-2 expression have no significant differences in various histopathological grading ovarianendometrioid carcinoma, and no correlation between Cyclin D1 and COX-2 expression all grade ovarian endometrioid carcinoma Latar belakangKarsinoma endometrioid ovarium mencakup 10-15% dari seluruh karsinoma ovarium, dan merupakan bentuk terbanyak kedua darikeganasan epitelial ovarium. Sel-sel epitel yang dipengaruhi COX-2 dan penghambatan apoptosis oleh perpanjangan fase G1 darisiklus sel melalui efek pada Cyclin D1 menunjukkan proliferasi lebih tinggi. Penelitian ini dilakukan tuntuk menganalisis korelasi danperbedaan ekspresi Cyclin D1 dan COX-2 pada berbagai grading karsinoma endometrioid.MetodePenelitian ini dilakukan secara observasional analitik dengan pendekatan cross sectional pada 23 sampel blok parafin di InstalasiPatologi Anatomik Rumah Sakit Dr. Soetomo. Sediaan mikroskopik diwarnai dengan antibodi Cyclin D1 dan COX-2, dievaluasi dandinilai dengan sistem skoring, serta dianalisis secara statistik menggunakan uji Kruskal Wallis dan Spearman.HasilTidak terdapat perbedaan ekspresi Cyclin D1 pada berbagai grading histopatologik, tidak terdapat perbedaan ekspresi COX-2 padaberbagai grading histopatologik, tidak terdapat hubungan ekspresi Cyclin D1 dan COX-2 pada semua grading histopatologik, tetapiterdapat hubungan antara ekspresi Cyclin D1 dan COX-2 pada grade 3 karsinoma endometrioid ovarium.KesimpulanKarsinoma endometrioid ovarium grade 1, 2, 3 menunjukkan ekspresi D1 dan COX-2 yang tidak berbeda bermakna. Sedangkanpada karsinoma endometrioid grade 1, grade 2, grade 3 menunjukkan tidak ada korelasi antara ekspresi cyclin D1 dan COX-2. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/421 10.55816/mpi.v29i2.421 Majalah Patologi Indonesia; Vol. 29 No. 2 (2020): MPI; 107 - 113 2527-9106 0215-7284 10.55816/mpi.v29i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/421/297

oai:ojs.pkp.sfu.ca:article/431 2024-05-31T08:30:02Z patologi:ART driver

oai:ojs.pkp.sfu.ca:article/432 2024-05-31T08:30:02Z patologi:ART driver

oai:ojs.pkp.sfu.ca:article/441 2022-05-19T12:45:02Z patologi:ART driver

Detection of Micrometastasis in Bulky Lymph Nodes Cervical Carcinoma Stage IA-IIB Using Cytokeratin AE1/AE3 Deteksi Mikrometastasis pada Bulky Kelenjar Getah Bening Karsinoma Serviks Stadium IA-IIB Menggunakan Sitokeratin AE1/AE3 Angeline, Reni Tjahjadi, Hartono Wuyung, Puspita Eka bulky lymph nodes; cervical carcinoma stage IA-IIB; cytokeratin AE1/AE3; micrometastasis bulky KGB; karsinoma serviks stadium IA-IIB; mikrometastasis; sitokeratin AE1/AE3. BackgroundCervical uterine carcinoma is a primary malignant disease of the uterine cervix. The presence or absence of metastasis in lymphnodes does not alter the stage but affects recurrence, survival, and therapy. Immunohistochemistry stains of the AE1/AE3cytokeratin can assist in the diagnosis and determine the small focus of carcinoma metastasis. In this study, we analyzedimmunohistochemistry cytokeratin AE1/AE3 could determine micrometastasis in bulky lymph nodes negative.MethodsThis study used cross-sectional design. The sample consisted of 52 cases of stage IA-IIB cervical carcinoma performed by radicalhysterectomy and lymphadenectomy accompanied by negative bulky lymph nodes at the Department of Anatomical PathologyFaculty of Medicine University of Indonesia/Cipto Mangunkusumo Hospital (FKUI/RSCM) from January 2011 to June 2017. Allcases stained by immunohistochemistry cytokeratin AE1/AE3.ResultsBulky Lymph Nodes Cervical Carcinoma stage IA-IIB, histopathologic diagnose of non keratinized squamous cell carcinoma,moderate differentiation degree, no lymphovascular invasion and deepest invasion >5.0 mm. Immunohistochemistry staining ofAE1/AE3 cytokeratin showed no micrometastasis in all cases.ConclusionThe immunohistochemistry staining of AE1/AE3 cytokeratin used in Bulky Lymph Nodes Cervical Carcinoma stage IA-IIB could notdetected micrometastasis in all cases. ABSTRAKLatar belakangKarsinoma serviks uteri adalah keganasan primer pada serviks uteri. Ada atau tidaknya metastasis pada kelenjar getah bening tidakmengubah stadium, namun mempengaruhi kekambuhan, kesintasan dan terapi. Pulasan imunohistokimia sitokeratin AE1/AE3dapat membantu dalam penegakkan diagnosis maupun menentukan fokus kecil metastasis karsinoma. Pada penelitian ini dianalisispemeriksaan imunohistokimia sitokeratin AE1/AE3 untuk menentukan mikrometastasis pada bulky KGB negatif.MetodePenelitian ini menggunakan desain potong lintang. Sampel terdiri atas 52 kasus karsinoma serviks stadium IA-IIB yang dilakukanhisterektomi radikal dan limfadenektomi yang disertai bulky KGB negatif di Departemen Patologi Anatomik Fakultas KedokteranUniversitas Indonesia/Rumah Sakit Dr. Cipto Mangunkusumo (FKUI/RSCM) periode Januari 2011 sampai Juni 2017. Dilakukanpulasan imunohistokimia sitokeratin AE1/AE3.HasilPada bulky KGB Karsinoma Serviks stadium IA-IIB diagnosis histopatologik karsinoma sel skuamosa (KSS) tidak berkeratin, derajatdiferensiasi sedang, tidak ada invasi limfovaskular dengan invasi terdalam >5,0 mm. Pemeriksaan imunohistokimia sitokeratinAE1/AE3 menunjukkan tidak adanya mikrometastasis pada seluruh kasus.KesimpulanPemeriksaan imunohistokimia sitokeratin AE1/AE3 yang digunakan pada bulky KGB karsinoma serviks stadium IA-IIB tidak dapatmendeteksi adanya mikrometastasis Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-09-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/441 10.55816/mpi.v29i3.441 Majalah Patologi Indonesia; Vol. 29 No. 3 (2020): MPI; 114 - 122 2527-9106 0215-7284 10.55816/mpi.v29i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/441/312

oai:ojs.pkp.sfu.ca:article/442 2022-05-19T12:45:21Z patologi:ART driver

The correlation between IGF-1R and HER-2 expression with mitosis count and histopathological grade in invasive breast carcinoma of no special type Hubungan Ekspresi IGF-1R dan HER-2 Terhadap Jumlah Mitosis dan Derajat Histopatologik pada Karsinoma Payudara Invasif Tidak Spesifik Setiawati, Yessy Asri, Aswiyanti Rasyid, Rosfita : Invasive breast carcinoma of NST, IGF-1R, HER-2. Karsinoma payudara invasif tidak spesifik, IGF-1R, HER-2 BackgroundThe IGF-1R signaling pathways have cross-talk with HER-2 signaling pathways and was thought to be one of the resistance mechanism intrastuzumab therapy. This research was proposed to analyze the correlation between IGF-1R and HER-2 expression with mitosis count andhistopathological grade in invasive breast carcinoma of no special type.MethodsThis research was a cross-sectional design. A total of fifty-five invasive breast carcinoma of no special type cases diagnosed in theAnatomical Pathology Centre Diagnostic of Medical Faculty of Andalas University in a period of 2014-2015 were collected and stainedimmunohistochemically with IGF-1R and HER-2 antibodies. IGF-1R and HER-2 expression were examined and their correlation with mitosiscount and histopathological grade were statistically analyzed with T-test, Oneway Anova and Chi-Square test.ResultsIGF-1R cytoplasm and membranous expression were found positive in 18.2% and 34.5% cases respectively, meanwhile, HER-2 expressionswere found positive in 23.6% cases of invasive breast carcinoma of no special type. There were significant correlations between IGF-1Rcytoplasm expression with mitosis count (p=0.049). There were no significant correlations between IGF-1R membranous expression withmitosis count (p=0.641) with histopathological grade (p=1.00). There were no significant correlations between HER-2 expression withmitosis count (p=0,495) and histopathological grade (p=1.000).ConclusionIGF-1R expressions have a significant correlation with mitosis count rather than HER-2 expressions. Inhibition it’s signaling pathways mayhave therapeutic value in breast carcinoma. Combination therapy of anti-HER-2 and anti-IGF-1R are expected to overcome resistance withtrastuzumab in HER-2 positive breast carcinoma ABSTRAKLatar belakangAdanya cross-talk antara jalur sinyal IGF-1R dengan HER-2 dan diduga menjadi salah satu mekanisme terjadinya resistensi terhadaptrastuzumab. Tujuan penelitian ini untuk menganalisis hubungan ekspresi IGF-1R dan HER-2 terhadap jumlah mitosis dan derajathistopatologik pada karsinoma payudara invasif tidak spesifik.MetodePenelitian ini merupakan penelitian observasional dengan desain cross sectional. Lima puluh lima kasus karsinoma payudara invasif tidakspesifik yang didiagnosis di Sentra Diagnostik Patologi Anatomik Fakultas Kedokteran Universitas Andalas periode tahun 2014-2015dikumpulkan, dilakukan pewarnaan imunohistokima IGF-1R dan HER-2. Ekspresi IGF-1R dan HER-2 dinilai serta dianalisis hubungannyadengan jumlah mitosis dan derajat histopatologik secara statistik dengan menggunakan T-test, Oneway Anova dan Chi-Square test. Ujistatistik dianggap bermakna jika nilai p<0,05.HasilEkspresi IGF-1R sitoplasma dan membran positif ditemukan masing-masing pada 18,2% dan 34,5% kasus sedangkan ekspresi HER-2positif ditemukan pada 23,6% kasus karsinoma payudara invasif tidak spesifik. Terdapat hubungan yang bermakna antara ekspresi IGF-1Rsitoplasma dengan jumlah mitosis (p=0,049). Akan tetapi, tidak terdapat hubungan yang bermakna antara ekspresi IGF-1R membrandengan jumlah mitosis (p=0,641). Tidak terdapat hubungan yang bermakna antara ekspresi IGF-1R membran dan sitoplasma denganderajat histopatologik (p=1,000) serta tidak terdapat hubungan yang bermakna antara ekspresi HER-2 dengan jumlah mitosis (p=0,495) danderajat histopatologik (p=1,000).KesimpulanEkspresi IGF-1R mempunyai hubungan yang bermakna dengan jumlah mitosis dibandingkan dengan HER-2. Penghambatan jalur sinyalIGF-1R dapat bermanfaat pada penatalaksanaan karsinoma payudara. Kombinasi terapi anti-HER-2 and anti-IGF-1R diharapkan dapatmengatasi resistensi trastuzumab pada karsinoma payudara HER-2 positif. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-09-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/442 10.55816/mpi.v29i3.442 Majalah Patologi Indonesia; Vol. 29 No. 3 (2020): MPI; 123 - 130 2527-9106 0215-7284 10.55816/mpi.v29i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/442/313

oai:ojs.pkp.sfu.ca:article/444 2022-05-19T12:45:40Z patologi:ART driver

Analysis of BRAF and TERT Expressions in Papillary Thyroid Carcinoma Cases Based on ATA Risk Stratification Analisis Ekspresi BRAF dan TERT pada Kasus Papillary Thyroid Carcinoma Berdasarkan Stratifikasi Risiko ATA Sekarputri,, Cempaka Harsa Hary Kusumastuti,, Etty Mastutik, Gondo papillary thyroid carcinoma, BRAF, TERT, ATA risk stratification papillary thyroid carcinoma, BRAF, TERT, stratifikasi risiko ATA BackgroundPapillary thyroid carcinoma (PTC) is the most common malignancy in thyroid, accounting for more than 80% of all thyroid cancer.Rrecurrences of the disease reach 30% cases. It has been widely observed that BRAF and TERT is associated with aggressivenessbehaviors in human cancer. Both are involved in the pathogenesis of PTC that may be used as targets for new therapies. AmericanThyroid Association (ATA) risk stratification can define the risk of recurrence in PTC. No marker has been found to determine therisk of PTC recurrence. The purpose of this study is to analyzed BRAF and TERT expression in ATA risk stratification system inPTC classic variant samples.MethodsThe methods of this study is an analytical observational study with cross sectional approach, conducted on 56 samples of PTCclassic variant selected from Department of Anatomical Pathology Dr. Soetomo General Hospital between January 2015-December2017 by determined criteria in ATA risk stratification groups of low risk, intermediate risk and high risk. The expression BRAF andTERT observed using immunohistochemical staining of Santa Cruz monoclonal antibody.ResultsThe result for each group based on ATA risk stratification groups of low risk were 13 samples (23.20%), intermediate risk were 25samples (44.62%) and high risk were 18 samples (32.12%).The correlation was assessed with Spearman’s rho correlation test. There is significant correlation BRAF (p=0.004) with a value ofr=0.374 and TERT(p=0.032) with a value of r=0.287 for ATA risk stratification.ConclusionBRAF and TERT expression showed significant correlation to ATA risk stratification in classic variant PTC. ABSTRAKLatar belakangPapillary thyroid carcinoma (PTC) adalah jenis keganasan tiroid terbanyak, lebih 80% dari kanker tiroid. Rekurensi mencapai 30%kasus. BRAF dan TERT adalah marker yang berkaitan dengan sifat agresif pada kanker, yang keduanya terlibat pada patogenesisPTC dan telah menjadi target baru untuk terapi molekular. Sistem stratifikasi risiko ATA dinilai dapat menentukan risiko rekurensipada PTC. Sampai saat ini belum ditemukan marker yang dapat digunakan untuk menentukan risiko rekurensi PTC. Tujuanpenelitian ini adalah menganalisis korelasi ekspresi BRAF dan TERT terhadap stratifikasi risiko ATA pada kasus PTC.MetodeMetode penelitian yang dilakukan adalah analitik observasional dengan pendekatan cross sectional pada 56 sampel PTC varianklasik di SMF Patologi Anatomik RSUD. Dr. Soetomo periode Januari 2015-Desember 2017 dengan parameter ATA yang terbagimenjadi kelompok risiko rendah, risiko sedang, risiko tinggi. Ekspresi BRAF dan TERT dinilai dengan pulasan imunohistokimiamenggunakan antibodi monoklonal Santa Cruz. Korelasi antar variabel dianalisis dengan uji korelasi Spearman’s rho.HasilPada penelitian ini didapatkan untuk masing-masing kelompok berdasarkan stratifikasi risiko ATA untuk risiko rendah sebanyak 13sampel (23,20%), risiko sedang sebanyak 25 sampel (44,62%), dan risiko tinggi sebanyak 18 sampel (32,12%). Ekspresi BRAF(p=0,004) dengan nilai r=0,374 dan TERT (p=0,032) dengan nilai r=0,287 terhadap sistem stratifikasi ATA menunjukkan korelasiyang bermaknaKesimpulan:Ekspresi BRAF dan TERT menunjukkan korelasi signifikan terhadap stratifikasi risiko ATA pada kasus PTC varian klasik. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-09-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/444 10.55816/mpi.v29i3.444 Majalah Patologi Indonesia; Vol. 29 No. 3 (2020): MPI; 131 - 138 2527-9106 0215-7284 10.55816/mpi.v29i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/444/314

oai:ojs.pkp.sfu.ca:article/445 2022-05-19T12:45:55Z patologi:ART driver

Clinico histopathological Characteristics and Immunofluorescent Studies of Bullous pemphigoid in Anatomical Pathology Department, Cipto Mangunkusumo General Hospital within 2011-2018 Karakteristik Klinikohistopatologik dan Profil Imunofluoresensi Kasus Pemfigoid bulosa di Departemen Patologi Anatomik Rumah Sakit Cipto Mangunkusumo Tahun 2011-2018 Kurniawan, Fetisari Arisanty, Riesye bullous pemphigoid, autoimmune bullous diseases pemfigoid bulosa, penyakit bulosa autoimun. BackgroundBullous pemphigoid is the most common autoimmune bullous disease. It is characterized by the presence of autoantibodies againsthemidesmosomal proteins in dermo-epidermal junction. Immune complexes subsequently induce skin blisters formation clinically, which willappear as subepidermal cleft histopathologically, and revealed as immune depositions immunologically. These three findings are required inorder to estabilsh the diagnosis of Bullous pemphigoid. This study was aimed to asses the clinico histopathological characteristics andimmunofluorescence studies of bullous pemphigoid in Anatomical Pathology Department, Cipto Mangunkusumo General Hospital.MethodsA cross sectional study was conducted in Anatomical Pathology Department ,Cipto Mangunkusumo General Hospital from January 2011 toDecember 2018. Clinical, histopathological, and direct immunofluorescence results were evaluated.ResultsDuring the last 8 years, bullous pemphigoid was the second most common form of autoimmune bullous diseases. The mean age of thesample was 60 years old. Women were affected more common than men (61.6%). Majority of patients present with blisters eruption (67.4%)and most commonly found in the lower extremities (48.8%). Histopathological examinations showed mostly a subepidermal cleft (89.5%)containing infiltrates of eosinophil, neutrophil, and lymphocyte. DIF staining demonstrated 31 cases (36.0%) with immune depositscharacteristic for bullous pemphigoid.ConclusionThe diagnosis of bullous pemphigoid is relied on a combination of clinical, histopathological, and direct immunofluorescence findings. ABSTRAKLatar belakangPemfigoid bulosa merupakan penyakit bulosa autoimun tersering. Penyakit ini ditandai oleh adanya ikatan antibodi terhadap proteinhemidesmosom di dermo-epidermal junction (DEJ). Kompleks imun yang terbentuk menyebabkan timbulnya manifestasi bula secara klinik,celah subepidermal pada pemeriksaan histopatologik, dan deposit imun pada pemeriksaan direct immunofluorescence (DIF). Ketigagambaran tersebut merupakan dasar dalam menegakan diagnosis Pemfigoid bulosa. Penelitian ini dilakukan untuk mengetahui karakteristikkliniko-histopatologik dan profil imunofluoresensi kasus pemfigoid bulosa yang ada di Departemen Patologi Anatomik Rumah Sakit CiptoMangunkusumo (RSCM).MetodePenelitian ini merupakan penelitian deskriptif dengan desain penelitian potong-lintang. Kasus pemfigoid bulosa diambil dari arsipDepartemen Patologi Anatomik RSCM dari kurun waktu Januari 2011 sampai Desember 2018. Parameter penilaian meliputi keteranganklinik, gambaran histopatologik, dan hasil DIF.HasilSelama kurun waktu 8 tahun di Departemen Patologi Anatomik RSCM, pemfigoid bulosa menempati urutan kedua terbanyak darikeseluruhan penyakit bulosa autoimun yang ditemukan. Rerata usia sampel adalah 60 tahun. Kasus lebih sering dijumpai pada perempuan(61,6%), umumnya datang dengan keluhan lesi lepuh/lenting (67,4%), dengan lokasi terbanyak dijumpai pada ekstremitas bawah (48,8%).Gambaran histopatologik umumnya menunjukkan celah terletak di subepidermal (89,5%) berisikan campuran sel-sel eosinofil, neutrofil, danlimfosit. Penelusuran hasil DIF menunjukkan 31 kasus (36,0%) dengan deposit imun yang sesuai dengan pemfigoid bulosa.KesimpulanPenegakan diagnosis pemfigoid bulosa membutuhkan kombinasi tiga pilar diagnostik meliputi keterangan klinik, gambaran histopatologik,dan DIF Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-09-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/445 10.55816/mpi.v29i3.445 Majalah Patologi Indonesia; Vol. 29 No. 3 (2020): MPI; 139 - 144 2527-9106 0215-7284 10.55816/mpi.v29i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/445/315

oai:ojs.pkp.sfu.ca:article/446 2022-05-19T12:46:17Z patologi:ART driver

Correlation between AgNOR Expression and Ki-67 Expression with Prostate Adenocarcinoma Grading Ekspresi AgNOR dan Ekspresi Ki-67 pada Grading Adenokarsinoma Prostat Rita, Roza Delyuzar Imelda Laksmi, Lidya AgNOR, Ki-67, Gleason grade group, prostate adenocarcioma. : AgNOR, Ki-67, gleason grade group, adenokarsinoma prostat. BackgroundProstate cancer is the second most common cancer and fifth most common cancer cause of death in males. Tissue biopsy is a goldstandard examination to diagnose prostate cancer. One of the hallmarks of cancer is increased activity of cell proliferation. Thisactivity can be detected with Ki-67 and AgNOR (Argyrophilic nucleolar organizing region). The aim of this study is to analyzedcorrelation between AgNOR and Ki-67 expression in grading of prostated adenocarcinoma.MethodsThis analytic cross-sectional study was held in Laboratory of Anatomical Pathology of Medical Faculty of USU/ RSUP H. AdamMalik Medan. Thirty paraffin blocks diagnosed with prostate adenocarcinoma were stained with H&E and p63 immunohistochemistrythen evaluated based on Gleason’s histopathological grading and stained with Agnor and Ki 67.ResultsAgNOR expression yang diperoleh pada grading adenokarsinoma prostat group 1, 2, 3, 4, dan 5 berturut-turut adalah 43; 32,40(±14,54); 64,29 (±28,2); 59,5 (±28,32); 69,22 (±29,26). Ekspresi Ki-67 pada setiap grading adenokarsinoma prostat group 1, 2, 3, 4,dan 5 secara berurutan adalah 43; 32,4 (±14,53); 64,29 (±28,2), 59,5 (±28,31); 69,22 (±29,26). Statistical analyses showed thatthere was no significantly correlation between grading of prostate adenocarcinoma and AgNOR expression (p=0.065), and Ki-67expression (p=0.18). Nevertheless, a significantly correlation between KI-67 expression and grading of prostate adenocarcinomawas found (p=0.34).ConclusionKi-67 could be used as prognostic indicator for prostate adenocarcinoma. ABSTRAKLatar belakangKanker prostat merupakan kanker terbanyak kedua dan penyebab kematian terbanyak kelima pada laki-laki. Biopsi jaringanmerupakan standar baku dalam menegakkan diagnosis kanker prostat. Salah satu ciri khas kanker adalah aktivitas proliferasi selyang meningkat. Aktivitas proliferasi ini dapat dilihat dengan menggunakan penanda Ki-67 dan AgNOR (Argyrophilic nucleolarorganizing region). Tujuan penelitian ini untuk menganalisis ekspresi AgNOR dan ekspresi Ki-67 pada grading adenokarsinomaprostat.MetodePenelitian ini merupakan studi uji analitik dengan desain potong lintang (cross sectional) yang dilakukan di Laboratorium PatologiAnatomik Fakultas Kedokteran Universitas Sumatera Utara/RSUP H. Adam Malik Medan. Tiga puluh sampel blok parafin yangdiagnosis sebagai adenokarsinoma prostat dari peawarnaan haematoksilin & eosin dan imunohistokimia p63, dinilai gradingberdasarkan gleason grade group dan diperiksakan dengan pewarnaan AgNOR dan Ki-67.HasilEkspresi AgNOR yang diperoleh pada grading adenokarsinoma prostat group 1, 2, 3, 4, dan 5 berturut-turut adalah 43; 32,40(±14,54); 64,29 (±28,2); 59,5 (±28,32); 69,22 (±29,26). Ekspresi Ki-67 pada setiap grading adenokarsinoma prostat group 1, 2, 3, 4,dan 5 secara berurutan adalah 43; 32,4 (±14,53); 64,29 (±28,2), 59,5 (±28,31); 69,22 (±29,26). Analisis statistik menunjukkankorelasi yang tidak signifikan antara grading adenokarsinoma prostat dan ekspresi AgNOR (p=0,07), dan ekspresi Ki-67 (p=0,18).Namun, terdapat hubungan yang signifikan antara ekspresi Ki-67 dan grading adenokarsinoma prostat (p=0,34).KesimpulanKi-67 dapat digunakan sebagai penanda prognostik untuk adenokarsinoma prostat Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-09-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/446 10.55816/mpi.v29i3.446 Majalah Patologi Indonesia; Vol. 29 No. 3 (2020): MPI; 145 - 150 2527-9106 0215-7284 10.55816/mpi.v29i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/446/316

oai:ojs.pkp.sfu.ca:article/447 2022-05-19T12:46:46Z patologi:ART driver

The Relationship between Inflammatory Cell Infiltration Density and the Clinicopathology of Colorectal Carcinoma in Dr. Mohammad Hoesin General Hospital-Palembang Hubungan Kepadatan Infiltrasi Sel Radang Limfosit dengan Klinikopatologik Karsinoma Kolorektal di RSUP Dr. Mohammad Hoesin Palembang Ienawi, Calvin Auline Rusminan, Suly Hafy, Zen TILs, Colorectal carcinoma, clinicopathological characteristics, lymphocytes density, cell progressivity, cell behavior. TILs, karsinoma kolorektal, karakteristik klinikopatologik, kepadatan limfosit, progresivitas sel, perilaku sel. BackgroundColorectal carcinoma has become one of primary problem that caused mortality and morbidity around the world. This malignancyhas contributed almost more than 9% of all cancer incidence. Lymphocyte infiltration density (TILs) is one of prognostic factors toevaluate colorectal cancer. Although some studies have found that there still have variations in sassociation lymphocyte density withclinicopathology of colorectal carcinoma which related to patient prognosis. This study was aimed to identify and analyze theassociation of lymphocyte density with clinicopathological colorectal carcinoma patients in Dr. Mohammad Hoesin General HospitalPalembang.MethodsA total of 41 HE stained slides which were diagnosed as colorectal carcinoma were observed under the light microscope.Lymphocytes were counted in five different areas that showed the highest lymphocytes density. The mean values of the lymphocytewere received from those five different areas. Determination of cutoff point was counted from the median of all mean value of thelymphocytes and being classified to become two categories, high-density lymphocytes and low-density lymphocytes.ResultsOut of 41 subjects, most patients have tumor size more than/or equal to 5 cm (63.4%), the tumors are often found in the left-sided ofcolon (39.0%), have moderate differentiated degree (65.9%), are included into T3 in tumor staging (48.8%), and no regional lymphnodes metastasis (39.0%). The most histopathological classification is adenocarcinoma (41.5%). Bivariate analysis showed thatlymphocyte density has correlation with tumor differentiation degree (p=0.021) and histopathological classification (p=0.004).ConclusionLymphocyte infiltration density has correlation with degree differentiation and histopathological classification of colorectal carcinomain Dr. Mohammad. Hoesin General Hospital-Palembang ABSTRAKPendahuluanKanker Kolorektal merupakan salah satu masalah utama penyebab morbiditas dan mortalitas di seluruh dunia. Keganasan inimenyumbang hampir lebih dari 9% seluruh angka kejadian kanker. Walaupun kepadatan infiltrasi limfosit (TILs) merupakan salahsatu faktor prognosis terhadap evaluasi kanker kolorektal, namun beberapa penelitian menemukan adanya variasi hubungan antarakepadatan limfosit dan klinikopatologik karsinoma kolorektal terkait dengan penentuan prognosis pasien. Penelitian ini bertujuanuntuk mengidentifikasi dan menganalisis hubungan antara kepadatan limfosit dan klinikopatologik pasien karsinoma kolorektal diRSUP Dr. Mohammad Hoesin Palembang.MetodeSebanyak 41 sediaan HE yang didiagnosis sebagai karsinoma kolorektal diamati di bawah mikroskop. Limfosit dihitung pada limaarea tumor yang berbeda yang menunjukkan kepadatan limfosit tertinggi. Nilai rerata limfosit didapatkan dari lima area tumortersebut. Penentuan nilai cutoff point diambil dari nilai median seluruh nilai rerata limfosit, kemudian diklasifikasikan menjadi duakategori, yaitu kepadatan limfosit tinggi dan kepadatan limfosit rendah.HasilDari 41 subjek penelitian, Sebagian pasien memiliki ukuran tumor yang lebih dari/sama dengan 5 cm (63,4%), sering dijumpai dikolon sinistra (39,0%), memiliki derajat diferensiasi sedang (65,9%), termasuk ke dalam kategori T3 (48,8%), dan belumbermetastasis ke limfonodus (39,0%). Klasifikasi histopatologik paling banyak adalah adenokarsinoma (41,5%). Analisis bivariatmenunjukkan bahwa kepadatan limfosit memiliki hubungan dengan derajat diferensiasi (p=0,021) dan klasifikasi histopatologiktumor (p=0,004).KesimpulanKepadatan infiltrasi limfosit memiliki hubungan dengan derajat diferensiasi dan klasifikasi histopatologik karsinoma kolorektal diRSUP Dr. Moh. Hoesin Palembang. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-09-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/447 10.55816/mpi.v29i3.447 Majalah Patologi Indonesia; Vol. 29 No. 3 (2020): MPI; 151 - 160 2527-9106 0215-7284 10.55816/mpi.v29i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/447/317

oai:ojs.pkp.sfu.ca:article/448 2022-05-19T12:47:04Z patologi:ART driver

The Expression of Programmed Death Ligand-1 (Pd-L1) in Testicular Seminoma as Potential Prognostic Factor Ekspresi Programmed Death Ligand-1 (PD-L1) pada Seminoma Testis sebagai Potensi Faktor Prognostik Boedijono, Freciyana Tanurahardja, Budiana Kodariah, Ria Seminoma, TILs, PD-L1, OS, PFS OS; PD-L1; PFS; seminoma; TILs BackgroundThe prognosis of testicular seminoma cases which failed to be cured with conventional therapy is poor. Several recurrent eventsafter chemotherapy were also found. PD-L1 is expressed in various types of malignancy and tumor infiltrating lymphocytes (TILs)and its role is known as a prognostic factor. This study was conducted to determine the role of PD-L1 expression seminoma indetermining overall survival (OS) and progression free survival (PFS).MethodsThis is a retrospective cohort study with survival analysis. Clinical data were obtained from medical record in RSUPN CiptoMangunkusumo since January 2011-December 2016 and observed for 2 years. Histopathological data were obtained fromAnatomical Pathology Department and PD-L1 immunohistochemical staining were performed.ResultsA significant correlation between PD-L1 expression in tumor cells with 2-year OS (p=0.023) and PFS (p=0.002) in testicularseminoma was found. No significant correlation between PD-L1 expression in TILs with 2-year OS (p=0.235) and PFS (p=0.111).We also found significant correlations between PD-L1 expression of tumor cells and TILs with PFS (p=0.019). A significantcorrelation between stage with 2-year OS (p=0.010) and PFS (p=0.000) and lymph node metastases with 2-year OS (p=0.010) andPFS (p=0.000).ConclusionPD-L1 expression of testicular seminoma cells were significantly correlated with OS and PFS. There were no statistically significantassociations between PD-L1 expression in TILs with OS and PFS. ABSTRAKLatar belakangSeminoma testis yang gagal sembuh dengan penatalaksanaan konvensional memiliki prognosis buruk. Beberapa kejadian rekurensetelah dilakukan kemoterapi juga ditemukan. Programmed Death Ligand-1 (PD-L1) terekspresi pada berbagai keganasan dantumor infiltrating lymphocytes (TILs) serta telah diketahui perannya sebagai faktor prognostik.MetodePenelitian ini dilakukan untuk mengetahui peran ekspresi PD-L1 pada seminoma testis dalam menentukan overall survival (OS) danprogression free survival (PFS). Penelitian ini merupakan penelitian kohort retrospektif dengan desain analisis kesintasan. Dataklinik diambil dari rekam medik RSUPN Dr.Cipto Mangunkusumo sejak Januari 2011-Desember 2016 yang diobservasi selama duatahun. Data histopatologik diambil dari Departemen Patologi Anatomik RSUPN Dr. Cipto Mangunkusumo yang kemudian dilakukanpulasan imunohistokimia PD-L1.HasilTerdapat hubungan yang bermakna antara ekspresi PD-L1 pada sel tumor dengan 2-year OS (p=0,023) dan PFS (p=0,002) padaseminoma testis. Tidak terdapat hubungan yang bermakna antara ekspresi PD-L1 pada TILs dengan 2-year OS (p=0,235) dan PFS(p=0,111).KesimpulanEkspresi PD-L1 pada sel tumor seminoma testis berhubungan dengan OS dan PFS, tetapi hubungan tersebut tidak ditemukan padaTILs Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-09-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/448 10.55816/mpi.v29i3.448 Majalah Patologi Indonesia; Vol. 29 No. 3 (2020): MPI; 161 - 171 2527-9106 0215-7284 10.55816/mpi.v29i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/448/318

oai:ojs.pkp.sfu.ca:article/449 2022-05-19T12:44:05Z patologi:Redaksi

Halaman Redaksi Redaksi MAJALAH PATOLOGI INDONESIAIndonesian Journal of Pathology Penerbitan ResmiPerhimpunan Dokter Spesialis Patologi Indonesia (IAPI)Indonesian Association of Pathologists Penanggung JawabKetua Umum PP-IAPI Pemimpin Umumdr. Totok Utoro, D.Med., SpPA(K)Mitra BestariProf. dr. Sutisna Himawan, SpPA(K), Prof. Dr. dr. Soeripto, SpPA(K),Prof. dr. Nadjib D. Lubis, SpPA(K), Prof. Dr. dr. Endang Joewarini, SpPA(K),Prof. dr. Mpu Kanoko, PhD., SpPA(K), Prof. dr. Syarifuddin Wahid, PhD., SpPA(K),Prof. Dr. dr. Ambar Mudigdo, SpPA(K), Dr. dr. Chairil Hamdani, SpPA(K),Prof. ``dr. Bethy S. Hernowo, PhD., SpPA(K), dr. Nurjati Chairani Siregar, MS., PhD., SpPA(K) Dewan RedaksiPemimpin RedaksiDr. Drs. Kusmardi, MS. Wakil Pemimpin Redaksidr. Endang SR. Hardjolukito MS., SpPA(K), drg. Agoeng Tjahajani, MS., SpPMM(K) Redaksidr. Ening Krisnuhoni, MS., SpPA(K), Dr. Dra. Puspita Eka Wuyung, MS., dr. Lia Damayanti, MS., SpPA.,dr. Maria Francisca Ham, PhD., SpPA(K)., Dr. dr. Indra Wijaya, SpPA(K)., Dr. dr. Lisnawati, SpPA(K).,dr. Budiana Tanurahardja, SpPA(K)., dr. Lidya Imelda Laksmi, SpPA., dr. Aswiyanti Asri, SpPA(K).,dr. Krisna Murti, M.Biotech., PhD., SpPA., dr. Meilania Saraswati, MPd.Ked., SpPA(K).,dr. Hermin Aminah Usman, SpPA(K)., Dr. dr. Udadi Sadhana, M.Kes., SpPA., Dr. drh. Gondho Mastutik, M.Kes., Dr. dr. Ni Putu Sriwidyani, SpPA., dr. Muh. Husni Cangara, SpPA., DFM. Sekretarisdr. Eka Susanto, SpPA. Alamat Redaksi dan Tata UsahaDepartemen Patologi Anatomik, Fakultas Kedokteran, Universitas IndonesiaJl. Salemba Raya No. 6, Jakarta 10430E-mail: mpi.iapi@gmail.com Versi on line: www.majalahpatologiindonesia.com Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2020-09-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/449 Majalah Patologi Indonesia; Vol. 29 No. 3 (2020): MPI 2527-9106 0215-7284 10.55816/mpi.v29i3

oai:ojs.pkp.sfu.ca:article/455 2022-05-19T12:36:22Z patologi:ART driver

Clinical Features and Histopathologic Characteristic of Thymomas Aspek Klinik dan Karakteristik Tipe Histopatologik Timoma Maria Mayella Vianney Lisnawati Rachmadi Masaoka-Koga; myasthenia gravis; thymoma. Masaoka-Koga staging system; myasthenia gravis; timoma. BackgroundThymoma is the most common neoplasm found in thymus gland and is classified into 5 main histopathological types, A, AB, B1, B2,and B3. Thymomas are most commonly found in the anterior mediastinum and accompanied with myasthenia gravis. The aim of thisstudy was to find out clinical feature and histopathological characteristics of thymomas in Department of Anatomical Pathology, Facultyof Medicine, University of Indonesia/Dr. Cipto Mangunkusumo Hospital (FKUI/RSCM) in 2014-2018.MethodsThis is a descriptive study, using a cross-sectional design. Clinical and histopathological data obtain from the medical records andarchives of the Department of Anatomical Pathology of FKUI/RSCM. Clinical data consists of age, sex, tumor location, clinicalsymptom, concomitant disease, metastasis, and treatment. Histopathological assessment consists of histopathology type using WorldHealth Organization criteria and staging using Masaoka-Koga staging system.ResultsThere were 31 thymoma cases matched the inclusion criteria. Nineteen (61.3%) of 31 cases were women. The mean age of thesubjects was 46.7 years old. A total of 12 cases (37.5%) had muscle weakness due to myasthenia gravis as chief complaint and 21cases (67.7%) of tumor mass in the anterior mediastinum. There 9 cases of type AB and 2 cases of type B2.ConclusionThymoma is more common in women with an average age of 46.7 years. The most common paraneoplastic syndrome is myastheniagravis and mostly found in the anterior mediastinum. The most common histopathologic type is type AB, while type B2 is the least. Latar belakangTimoma adalah neoplasma tersering yang ditemukan pada organ timus dan diklasifikasikan menjadi 5 tipe histopatologik utama, yaituA, AB, B1, B2, dan B3. Timoma paling sering ditemukan di mediastinum anterior dan disertai dengan sindrom paraneoplastikmyasthenia gravis. Penelitian ini ingin mengetahui aspek klinik dan karakteristik tipe histopatologik timoma di Departemen PatologiAnatomik Fakultas Kedokteran Universitas Indonesia/Rumah Sakit Dr. Cipto Mangunkusumo (PA-FKUI/RSCM) tahun 2014-2018.MetodePenelitian ini merupakan penelitian deskriptif dengan desain penelitian potong-lintang. Data klinik dan histopatologik berasal darirekam medik dan arsip Departemen Patologi Anatomik FKUI/RSCM tahun 2014-2018. Data klinik berupa usia, jenis kelamin, lokasitumor, gejala klinik, penyakit penyerta, metastastis, dan terapi. Penilaian histopatologik meliputi diagnosis tipe histopatologikberdasarkan kriteria World Health Organization dan stadium berdasarkan Masaoka-Koga staging system.HasilDitemukan 31 kasus timoma yang sesuai dengan kriteria inklusi. Dari 31 kasus, 19 (61,3%) di antaranya adalah perempuan. Reratausia subyek adalah 46,7 tahun. Sebanyak 12 kasus (37,5%) memiliki gejala klinik kelemahan otot akibat myasthenia gravis dan 21kasus (67,7%) massa tumor di mediastinum anterior. Ditemukan 9 kasus timoma tipe AB dan 2 kasus timoma tipe B2. Sebanyak 3kasus berada di stadium IV dengan metastasis ke kelenjar getah bening dan paru.KesimpulanTimoma lebih banyak ditemukan pada wanita dengan rerata usia 46,7 tahun. Sindrom paraneoplastik tersering adalah myastheniagravis dan lokasi tersering adalah mediastinum anterior. Tipe histopatologik yang paling banyak ditemukan adalah tipe AB, sedangkanyang paling sedikit ditemukan adalah tipe B2 Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/455 10.55816/mpi.v30i1.455 Majalah Patologi Indonesia; Vol. 30 No. 1 (2021): MPI; 172 - 179 2527-9106 0215-7284 10.55816/mpi.v30i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/455/319

oai:ojs.pkp.sfu.ca:article/456 2022-05-19T12:36:41Z patologi:ART driver

CK19 and HBME-1 Expression as Diagnostic Test in Benign and Malignant Thyroid Lesions Uji Diagnostik Ekspresi CK19 dan HBME-1 pada Lesi Jinak dan Ganas Tiroid Indah Yati Henny Sulastri Aspitriani NH, FA,PTC, FC, CK19 and HBME-1 HN, AF, KPT, KFT, CK19 dan HBME-1 BackgroundThyroid carcinoma is the most common malignancy of the endocrine system. Some cases histopathologically have overlappingmorphological features among papillary thyroid carcinoma follicular variants and follicular adenomas; beetween nodular hyperplasiaand protruding papillary protrusion; nuclear feature and thyroid papillary carcinoma. The combination of CK19 and HBME-1imunohistochemical staining have been proven as specific and sensitive markers to distinguish benign and malignant lesions thyroid.The purpose of this study was to determine the differences in CK19 and HBME-1 expression in benign and malignant Thyroid lesions.MethodsThis study used a diagnostic test of 50 cases (16 cases of nodular hyperplasia, 8 cases of follicular adenoma, 21 papillary thyroidcarcinomas and 5 cases of thyroid follicular carcinoma) clinicopathological data were noted. Immunohistochemical staining wasperformed using CK19 and HBME-1antibodies and the their expression assessed. Data were analysed by SPSS version 22.0.ResultsBased on the four histopathological diagnoses, the majority patient, were females between 20-50 years.Fifty percent of macroscopicsample size was more than 5 cm. The expression of CK19 and HBME-1 has a sensitivity value of 76.9% and 92.3% and a specificityvalue of 79.2% and 79.2%, consecutively. While positive predictive value 80% and 82.8% and negative predictive value 76% and90.5%, accuracy of 78% and 86%, respectively.ConclusionHBME-1 expression could be used to distinguishing benign from malignan lesions of thyroid with high diagnostic sensitivity thanCK19 expression. ABSTRAKLatar belakangKarsinoma tiroid merupakan keganasan tersering sistem endokrin. Secara histopatologi sering dijumpai morfologi yang tumpangtindih antara karsinoma papiler tiroid varian folikular dan adenoma folikular; antara nodular hiperplasia dan penonjolan papiler yangtipis; serta antara gambaran inti jernih dan karsinoma papiler tiroid. CK19 dan HBME-1 merupakan penanda kombinasi yang telahdibuktikan sebagai penanda spesifik dan sensitif dalam membedakan lesi jinak dan lesi ganas pada tiroid. Tujuan penelitian ini untukmengetahui perbedaan ekspresi CK19 dan HBME-1 pada lesi jinak dan ganas tiroid.MetodePada penelitian ini dilakukan uji diagnostik, dengan sampel penelitian berjumlah 50 kasus, yang terdiri atas 16 kasus hiperplasianodular (HN), 8 kasus adenoma folikular (AF), 21 kasus karsinoma papiler tiroid (KPT) dan 5 kasus karsinoma folikular tiroid (KFT).Pemeriksaan imunohistokimia dilakukan dengan antibodi CK19 dan HBME-1 dan dilanjutkan dengan penilaian ekspresi kedua proteintersebut.HasilBerdasarkan keempat diagnosis histopatologi mayoritas usia terbanyak 20-50 tahun dengan jenis kelamin perempuan dan ukuranjaringan makroskopik tiroid >5 cm. Ekspresi CK19 dan HBME-1 masing-masing memiliki nilai sensitivitas 76,9% dan 92,3% dan nilaispesifisitas 79,2% dan 79,2%, nilai positive predictive value (PPV) 80% dan 82,8% dan negative predictive value (NPV) 76% dan90,5%, akurasi 78% dan 86%.KesimpulanEkspresi HBME-1 dapat digunakan untuk membedakan lesi jinak dan lesi ganas tiroid dengan nilai sensitivitas diagnostik yangtinggi dibandingkan dengan ekspresi CK19. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/456 10.55816/mpi.v30i1.456 Majalah Patologi Indonesia; Vol. 30 No. 1 (2021): MPI; 180 - 188 2527-9106 0215-7284 10.55816/mpi.v30i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/456/320

oai:ojs.pkp.sfu.ca:article/458 2024-02-22T03:53:26Z patologi:ART driver

The Association between EGFR Expressions with Histopathological Grade and Perineural Invasion in Prostatic Adenocarcinoma Hubungan antara Ekspresi Epidermal Growth Factor Receptor (EGFR) dengan Derajat Histopatologik dan Invasi Perineural pada Adenokarsinoma Prostat Ria Oktavia R. Zuryati Nizar Yenita Tofrizal prostatic adenocarcinoma, EGFR expression, histopathology grade, perineural invasion adenokarsinoma prostat, ekspresi EGFR, derajat histopatologik, invasi perineural ABSTRAKLatar belakangEpidermal Growth Factor Receptor (EGFR) berperan penting pada karsinogenesis beberapa karsinoma termasuk karsinoma prostat.Overekspresi EGFR pada adenokarsinoma menyebabkan sel tumor menjadi resisten terhadap terapi hormonal. Tujuan penelitian iniuntuk mengetahui hubungan antara ekspresi EGFR dengan derajat histopatologik dan invasi perineural pada adenokarsinomaprostat.MetodePenelitian ini merupakan penelitian observasional dengan desain cross sectional dengan sampel penelitian sebanyak 60 kasusadenokarsinoma prostat yang telah didiagnosis di 5 Laboratorium Patologi Anatomik di Sumatera Barat periode Januari 2015 sampaiDesember 2017. Sampel diperoleh dari blok parafin yang berasal dari jaringan hasil operasi transuretral resection of prostat (TURP)dan prostatektomi kemudian dilakukan reevaluasi terhadap derajat histopatologik dan invasi perineural. Ekspresi EGFR pada seltumor dilihat dengan pewarnaan imunohistokimia. Analisis statistik menggunakan uji Chi-Square.HasilEkspresi EGFR positif ditemukan pada 38 kasus (63,33%) dan kasus terbanyak dengan EGFR positif adalah poorly differentiated(57,9%). Kasus adenokarsinoma prostat dengan invasi perineural paling banyak ditemukan dengan ekspresi EGFR positif (42,1%).Analisis statistik menunjukkan terdapat hubungan bermakna antara ekspresi EGFR dengan derajat histopatologik (p=0,003) dan tidakterdapat hubungan yang bermakna antara ekspresi EGFR dengan invasi perineural (p=0,129).KesimpulanTerdapat hubungan yang bermakna antara ekspresi EGFR dengan derajat histopatologik pada adenokarsinoma prostat dan tidakterdapat hubungan antara ekspresi EGFR dengan invasi perineural. ABSTRAKLatar belakangEpidermal Growth Factor Receptor (EGFR) berperan penting pada karsinogenesis beberapa karsinoma termasuk karsinoma prostat.Overekspresi EGFR pada adenokarsinoma menyebabkan sel tumor menjadi resisten terhadap terapi hormonal. Tujuan penelitian iniuntuk mengetahui hubungan antara ekspresi EGFR dengan derajat histopatologik dan invasi perineural pada adenokarsinomaprostat.MetodePenelitian ini merupakan penelitian observasional dengan desain cross sectional dengan sampel penelitian sebanyak 60 kasusadenokarsinoma prostat yang telah didiagnosis di 5 Laboratorium Patologi Anatomik di Sumatera Barat periode Januari 2015 sampaiDesember 2017. Sampel diperoleh dari blok parafin yang berasal dari jaringan hasil operasi transuretral resection of prostat (TURP)dan prostatektomi kemudian dilakukan reevaluasi terhadap derajat histopatologik dan invasi perineural. Ekspresi EGFR pada seltumor dilihat dengan pewarnaan imunohistokimia. Analisis statistik menggunakan uji Chi-Square.HasilEkspresi EGFR positif ditemukan pada 38 kasus (63,33%) dan kasus terbanyak dengan EGFR positif adalah poorly differentiated(57,9%). Kasus adenokarsinoma prostat dengan invasi perineural paling banyak ditemukan dengan ekspresi EGFR positif (42,1%).Analisis statistik menunjukkan terdapat hubungan bermakna antara ekspresi EGFR dengan derajat histopatologik (p=0,003) dan tidakterdapat hubungan yang bermakna antara ekspresi EGFR dengan invasi perineural (p=0,129).KesimpulanTerdapat hubungan yang bermakna antara ekspresi EGFR dengan derajat histopatologik pada adenokarsinoma prostat dan tidakterdapat hubungan antara ekspresi EGFR dengan invasi perineural. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/458 10.55816/mpi.v30i1.458 Majalah Patologi Indonesia; Vol. 30 No. 1 (2021): MPI; 189 - 196 2527-9106 0215-7284 10.55816/mpi.v30i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/458/418 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/460 2022-05-19T12:37:21Z patologi:ART driver

Relationship of Interleukin-17 Expression and Ki67 Proliferative Index in Breast Carcinoma Hubungan Ekspresi Interleukin-17 dan Indeks Proliferasi Ki67 pada Karsinoma Payudara Ika Kartika, Theodorus Citra Dewi Nyiayu Fauziah Kurniawati breast carcinoma, IL-17, Ki67, proliferation index : karsinoma payudara, IL-17, Ki67, indeks proliferasi BackgroundTumor cells have the ability to change the phenotype of inflammatory cells in the tumor environment into protumoral factors. One ofthe mediators produced such as interleukin 17 (IL-17) also plays a role in increasing tumor proliferation and invasion. Similar to IL-17,Ki67 has generally been used as a marker to assess the extent of breast carcinoma proliferation. This is the first study to determinethe relationship of IL-17 expression with Ki67 proliferation index of breast carcinoma patients.MethodsThis is a case series study using 38 paraffin block samples of breast carcinoma patients. The clinicopathological characteristicsdocumented are age, histopathological subtype and grade, TILs (tumor infiltrating lymphocytes) and molecular subtypes. Interleukin17 and Ki67 expressions were assessed using immunohistochemistry examination and data were analysed using the Pearson andSpearman test to assess correlations between the two variables.ResultsGeneral characteristics of the sample in this study were breast cancer patients aged >40 years (73.7%), histopathological subtypesof non-specific invasive carcinoma (84.2%), high tumor rates (78.9%), non-dominant TILs (94.7%) and molecular subtype luminal B(55.3%). There is no relationship between IL-17 expression and Ki67 proliferation index (p 0.72 and OR of 1.417), even though lowerexpression of IL-17 showed a higher Ki67 proliferation index.ConclusionExpression of IL-17 cannot describe the Ki67 proliferation index in breast carcinoma. Studies using another analytical techniques andlarge sample size are suggested to obtain more accurate results. ABSTRAKLatar belakangSel tumor memiliki kemampuan untuk dapat merubah fenotipe sel radang pada lingkungan tumor menjadi faktor protumoral. Bahkansalah satu mediator radang yang dihasilkan seperti interleukin 17 (IL-17) juga berperan dalam meningkatkan proliferasi dan dayainvasi tumor. Sama halnya dengan IL-17, Ki67 secara umum telah dipakai sebagai penanda untuk menilai tingkat proliferasi karsinomapayudara. Penelitian ini adalah penelitian pertama yang bertujuan untuk mengetahui hubungan ekspresi IL-17 dengan indeksproliferasi Ki67 pasien karsinoma payudara.MetodePenelitian ini adalah serial kasus yang menggunakan 38 sampel blok paraffin penderita karsinoma payudara. Karakteristikklinikopatologi didokumentasikan yaitu usia, subtipe dan derajat histologik, TILs (tumor infiltrating lymphocytes) dan subtipemolekular. Ekspresi IL-17 dan Ki67 dinilai menggunakan pulasan imunohistokimia dan analisis data menggunakan uji Pearson danSpearman untuk menilai korelasi antar kedua variabel.HasilKarakteristik umum sampel pada penelitian ini adalah penderita kanker payudara usia >40 tahun (73,7%), subtipe histopatologikarsinoma invasif tidak spesifik (84,2%), derajat histologik tumor tinggi (78,9%), TILs yang tidak dominan (94,7%) dan subtipemolekular lumninal B (55,3%). Tidak terdapat hubungan antara ekspresi IL-17 dan indeks proliferasi Ki67 (p 0,72 dan OR 1,417),walaupun terlihat kecenderungan rendahnya ekspresi IL-17 memberikan gambaran ekspresi Ki67 yang tinggi.KesimpulanEkspresi IL-17 belum dapat menggambarkan indeks proliferasi Ki67 pada karsinoma payudara. Penelitian menggunakan teknikanalisa dan jumlah sampel yang besar disarankan untuk mendapatkan hasil yang lebih akurat Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/460 10.55816/mpi.v30i1.460 Majalah Patologi Indonesia; Vol. 30 No. 1 (2021): MPI; 197 - 202 2527-9106 0215-7284 10.55816/mpi.v30i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/460/322

oai:ojs.pkp.sfu.ca:article/461 2022-05-19T12:39:26Z patologi:ART driver

Analyzes of Interleukin-17 Expression and Clinicopathological Characteristics of Breast Carcinoma Analisis Ekspresi Interleukin-17 pada Karakteristik Klinikopatologi Karsinoma Payudara Nyiayu Fauziah Kurniawati Ika Kartika Citra Dewi Theodorus breast cancer, IL-17, subtype, histopathological grade, TILs, lymphovascular invasion, molecular subtype. karsinoma payudara, IL-17, subtipe, grade histopatologi, TILs, invasi limfovaskular, subtipe molekuler BackgroundInteraction between cancer cells and tumor microenvironment plays roles in cancer proliferation and invasiveness. Many type ofcytokine produced by inflammatory cells in tumor stroma could stimulate tumor proliferation and invasiveness, such as interleukin-17.The aim of this study is to determine the correlation between IL-17 expression and clinicopathological characteristics of breast cancer.MethodsSerial cases study was performed by enrolling 40 paraffin block of breast cancer patients. Clinicopathological characteirstics werenoted, including age, histopathological subtype and grade, TILs, lymphovascular invasion and molecular subtype. TILs were scoredby denomination area of stromal tissue occupied by mononuclear cells over total stromal area. Expression of IL-17 was examined byusing immunohistochemistry with anti-IL-17 antibody and counted from stromal mononuclear cells cytoplasm staining from five areas.The average value was used as a cut-off point in determining level of expression. Data were analysed by using SPSS v.22.ResultsMajority of the patients were more than 40 years old (75%), histopathological grade III (77.5%), without lymphovascular invasion(62.5%), luminal B molecular subtype (57.5%), and non-dominant TILs (95%). IL-17 expression was not significantly correlated withclinicopathological characteristics with p value=0.079, 0.600, 0.739, 0.883 and 1.000 respectively for age, histological grade,lymphovascular invasion, molecular subtype and TILs.ConclusionInterleukin-17 expression and clinicopathological on characteristics of breast carcinoma between histopathological grade, TILs, andlymphovascular invasion showed no significant differences. There was a tendency that the higher IL-17 expression, the higherhistopathological grade, the lower TILs and positive lymphovascular invasion ABSTRAKLatar belakangInteraksi antara sel-sel tumor ganas dan lingkungan mikro tumor memainkan peran penting dalam proliferasi dan progresivitas selsel tumor. Beberapa sitokin yang dihasilkan oleh sel-sel radang pada stroma tumor dapat menstimulasi proliferasi dan invasi tumordiantaranya adalah interleukin-17. Penelitian ini bertujuan untuk menganalisis ekspresi IL-17 pada gambaran klinikopatologikarsinoma payudara antara subtype, grade histopatologik, dan invasi limfovaskular, TILs dan subtipe molekular.MetodePenelitian ini adalah serial kasus yang menggunakan 40 sampel blok parafin penderita karsinoma payudara. Karakteristikklinikopatologi didokumentasikan yaitu usia, subtipe dan grade histopatologi, invasi limfovaskular, TILs dan subtipe molekular. TILsdihitung sebagai luas area stroma yang berisi sel mononuklir banding luas total area stroma. Ekspresi IL-17 dinilai menggunakanpulasan imunohistokimia dengan antibodi anti-IL-17 pada semua sitoplasma sel mononuklear pada stroma di 5 area terpilih. Nilairata-rata digunakan sebagai cut-off dalam menentukan tinggi rendahnya ekspesi. Data dianalisis menggunakan SPSS v.22.HasilRata-rata usia pasien adalah 46,88 tahun (28-73 tahun), mayoritas histopathological grade III (77,5%), tanpa disertai invasilimfovaskular (62,5%), tergolong dalam subtipe molekular luminal B (57,5%) dan memiliki kepadatan TILs yang tidak dominan (95%).Didapatkan hasil yang tidak bermakna antara ekspresi IL-17 dengan semua karakteristik klinikopatologi dengan nilai p masing-masing0,079, 0,600, 0,739, 0,883 dan 1,000 untuk usia, grade, invasi limfovaskular, subtipe molekular dan TILs.KesimpulanEkspresi IL-17 pada karakteristik klinikopatologi karsinoma payudara antara grade histopatologi, TILs dan invasi limfovaskularmenunjukkan perbedaan tidak bermakna. Terdapat kecenderungan semakin tinggi ekspresi IL-17 semakin tinggi subtipe, gradehistopatologi, semakin rendah TILs dan positif invasi limfovaskular dan subtipe molekuler. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/461 10.55816/mpi.v30i1.461 Majalah Patologi Indonesia; Vol. 30 No. 1 (2021): MPI; 203 - 210 2527-9106 0215-7284 10.55816/mpi.v30i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/461/324

oai:ojs.pkp.sfu.ca:article/462 2022-05-19T12:39:50Z patologi:ART driver

Correlation between CD133 and EGFR Expression with Grading of Ovarian Carcinoma Hubungan Ekspresi CD133 dan EGFR Terhadap Derajat Keganasan pada Karsinoma Ovarium Agung Dwi Suprayitno Dyah Fauziah Gondo Mastutik ovarian carcinoma, CD133, EGFR. karsinoma ovarium, CD133, EGFR BackgroundOvarian carcinoma account for 90% of all malignant ovarian tumors. Epidermal Growth Factor Receptor (EGFR), also known asErbB1/HER1, has an important role in tumor cell proliferation. CD133 is a marker for hematopoietic stem cell and it is involved in tumorcell proliferation, self renewal and cell differentiation. The objective to analyze the expression of CD 133 and EGFR in high grade andlow grade ovarian carcinoma, and to determine the correlation between the expression of CD133 and EGFR expression in ovariancarcinoma.MethodsThe study was conducted with observational and cross sectional method on paraffin blocks of patients diagnosed as ovariancarcinoma from 1 January until 31 December 2017 in Dr. Soetomo Hospital. Ovarian carcinoma was divided into 2 grade, low gradeand high grade. Immunohistochemistry for EGFR and CD133 was performed. Immuno-reactive Score (IRS) was applied to evaluatethe expression of EGFR and CD133. The difference expression of CD133 and EGFR in low grade and high grade ovarian carcinomawas analyzed with Mann-Whitney test. Correlation between CD133 with EGFR was analyzed with Spearman test.ResultsThere were significant difference in expression of CD133 (p=0.0001) and EGFR (p=0.0005) in high grade and low grade OvarianCarcinoma. There was significant positive correlation between CD133 and EGFR expression in Ovarian Carcinoma (p=0.035; r=0.37)ConclusionExpression of CD133 and EGFR were higher in high grade ovarian carcinoma. The higher expression of CD133 will increaseexpression of EGFR in ovarian carcinoma. Both markers may be used as prognostic factor. ABSTRAKLatar belakangKarsinoma ovarium mencakup 90% dari keganasan pada organ ovarium. Epidermal Growth Factor Receptor (EGFR), dikenal sebagaiErbB1/HER1, mempunyai peran penting dalam proliferasi sel tumor. CD133 adalah penanda sel induk hematopoietic, proliferasi, selfrenewal dan diferensiasi sel. Tujuan penelitian ini untuk menganalisis perbedaan ekspresi CD133 dan EGFR pada karsinoma ovariumderajat tinggi dan derajat rendah serta menganalisis hubungan antara ekspresi CD133 dan EGFR karsinoma ovarium.MetodePenelitian dilakukan dengan metode observasi dan cross sectional pada blok parafin pasien dengan diagnosis karsinoma ovariumsejak 1 Januari sampai 30 Desember 2017 di Rumah Sakit dr. Soetomo. Karsinoma ovarium dibagi dalam 2 derajat, yaitu derajatrendah dan derajat tinggi. Dilakukan pemeriksaan imunohistokimia EGFR dan CD133. Ekspresi EGFR dan CD133 dievaluasi denganimunoreactive score (IRS). Perbedaan ekspresi CD133 dan EGFR pada karsinoma ovarium dianalisis menggunakan uji Man-Whitney.Hubungan antara ekspresi CD133 dan EGFR pada karsinoma ovarium dianalisis menggunakan uji Spearman.HasilTerdapat perbedaan ekspresi CD133 dengan p < 0,01 dan EGFR dengan p < 0,01 pada karsinoma ovarium derajat tinggi dan rendah.Terdapat korelasi positif yang signifikan antara ekspresi CD133 dan EGFR pada karsinoma ovarium (p=0,035; r=0,37).KesimpulanEkspresi CD133 dan EGFR lebih tinggi pada karsinoma ovarium derajat tinggi. Ekspresi CD133 tinggi akan meningkatkan ekspresiEGFR pada karsinoma ovarium. Kedua petanda ini dapat digunakan sebagai faktor prognostik Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/462 10.55816/mpi.v30i1.462 Majalah Patologi Indonesia; Vol. 30 No. 1 (2021): MPI; 211 - 218 2527-9106 0215-7284 10.55816/mpi.v30i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/462/325

oai:ojs.pkp.sfu.ca:article/463 2022-05-19T12:40:06Z patologi:ART driver

Association Between Clinicopathologic Characteristics and WHO Prognostic Group in Gastrointestinal Stromal Tumor Patients at RSUP Dr. Mohammad Hoesin, Palembang Hubungan Antara Karakter Klinikopatologik dengan Grup Prognostik WHO pada Pasien Gastrointestinal Stromal Tumor di RSUP Dr. Mohammad Hoesin, Palembang Nur Azizah Suly Auline Rusminan Subandrate clinicopathologic characteristics, gastrointestinal stromal tumor, GIST, WHO prognostic group. gastrointestinal stromal tumor, GIST, grup prognostik WHO, karakter klinikopatologik. BackgroundGastrointestinal stromal tumors are the most common mesenchymal tumors in the gastrointestinal tract. Based on tumor size andmitotic index, WHO classified GIST into three prognostic groups which were benign, uncertain malignant potential, and malignant.Some studies revealed that there was variation in association between age, gender, tumor location, histopathologic type and prognosisof GIST patients. This study aimed to identify and analyze the association between clinicopathologic characteristics and WHOprognostic group in GIST patients at RSUP Dr. Mohammad Hoesin Palembang.MethodsThis study used 30 medical records of GIST patients during 01 January 2014 to 27 December 2018 in the Department of AnatomicPathology RSUP Dr. Mohammad Hoesin. Clinicopathologic characteristics and WHO prognostic group data were collected. Theassociation between clinicopathologic characteristics (age, gender, tumor location, histopathologic type) and WHO prognostic groupwere analyzed by chi-square test.ResultsFrom 30 subjects, 40% of the patients aged 52-58 years.There was no gender predilection. A total of 33.3% of the GISTs were locatedin the small intestine. Majority of histopathologic type was spindle cell type (93.3%). Bivariate analysis showed there were no significantassociation between clinicopathologic characteristics: age (p=0.811), gender (p=0.682), tumor location (p=1.000), histopathologic type(p=0.469) and WHO prognostic group.ConclusionThere was no significant association between clinicopathologic characteristics and WHO prognostic group in GIST patients at RSUPDr. Mohammad Hoesin Palembang. We suggest doing a cohort study about association between clinicopathologic characteristics andsurvival rate in GIST patients. ABSTRAKLatar belakangGastrointestinal stromal tumor merupakan tumor mesenkim tersering pada saluran pencernaan. WHO mengklasifikasikan GISTmenjadi tiga grup prognostik berdasarkan ukuran dan indeks mitosis tumor, yaitu jinak, uncertain malignant potential, dan ganas.Beberapa penelitian menemukan adanya variasi hubungan usia, jenis kelamin, lokasi tumor, dan tipe histopatologi dengan prognosispasien GIST. Penelitian ini bertujuan untuk mengidentifikasi dan menganalisis hubungan antara karakter klinikopatologi dengan grupprognostik WHO pada pasien GIST di RSUP Dr. Mohammad Hoesin Palembang.MetodePenelitian ini menggunakan 30 rekam medis pasien yang telah didiagnosis GIST di Departemen Patologi Anatomik RSUP Dr.Mohammad Hosein periode 01 Januari 2014-27 Desember 2018. Dilakukan pengumpulan data klinikopatologik dan grup prognostikpasien GIST. Hubungan karakter klinikopatologik yang meliputi usia, jenis kelamin, lokasi tumor, dan tipe histopatologik dengan grupprognostik WHO dianalisis menggunakan uji chi-square.HasilDari 30 subjek penelitian, 40% pasien berusia 52-58 tahun dan tidak ditemukan perbedaan distribusi berdasarkan jenis kelamin.Lokasi tumor terbanyak dijumpai pada usus halus (33,3%). Mayoritas tipe histopatologik yang ditemukan adalah sel spindel (93,3%).Hasil analisis bivariat menunjukkan tidak ada hubungan bermakna antara karakter klinikopatologik berupa usia (p=0,811), jeniskelamin (p=0,682), lokasi tumor (p=1,000), dan tipe histopatologik (p=0,469) dengan grup prognostik WHO.KesimpulanTidak terdapat hubungan antara karakter klinikopatologik berupa usia, jenis kelamin, lokasi tumor, dan tipe histopatologik dengangrup prognostik pasien GIST di RSUP Dr. Mohammad Hoesin, Palembang. Sebagai saran diusulkan penelitian dengan desain kohortuntuk mengetahui hubungan antara karakter klinikopatologik dengan angka harapan hidup pada pasien GIST. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-01-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/463 10.55816/mpi.v30i1.463 Majalah Patologi Indonesia; Vol. 30 No. 1 (2021): MPI; 219 - 226 2527-9106 0215-7284 10.55816/mpi.v30i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/463/326

oai:http://majalahpatologiindonesia.com/p/index.php/patologi/oai:article/464 2021-02-28T13:52:23Z patologi:Redaksi driver

oai:ojs.pkp.sfu.ca:article/466 2022-05-19T12:19:35Z patologi:ART driver

Density of CD8 in Tumor Microenvironment of Diffuse Large B Cell Lymphoma Subtypes Kepadatan CD8 di Lingkungan Mikrotumor pada Subtipe Diffuse Large B Cell Lymphoma Yunalda, Indah Murt, Krisna Farida, Aida Kartika, Ika Bahar, Erial DLBCL, CD8, GCB, non-GC DLBCL GCB, DLBCL non-GCB, TILs CD8 BackgroundDiffuse Large B Cell Lymphomas (DLBCL) is divided into two subtypes, GCB and non-GCB, which are determined based on Hans'salgorithm. The GCB subtype has a better prognosis than non-GCB. Some studies indicate that the tumor progression wasinfluenced by tumor microenvironment, including CD8+ TILs located both surrounding tumor cells and peripheral. This study aims todetermine the density of CD8+ TILs in the tumor microenvironment of DLBCL subtypes.MethodsA Cross sectional study of 40 samples of DLBCL registered to Department of Anatomical Pathology Faculty of Medicine UniversitasSriwijaya/Dr. Mohammad Hoesin Palembang Hospital from 1st October 2017 to 31st December 2018. All samples were stained usinganti-CD8 antibody, afterward, lymphocytes express CD8 were assessed both in surrounding tumor cells and peripheral. The densityof CD8 in each DLBCL subtype was analyzed statistically using Mann Whitney U Test.ResultsIn this study, non-GCB subtype was found higher in comparison to GCB subtype (72.5%) and the samples was dominated by youngages (75%). Significantly, peripheral CD8 density was higher than that of surrounding tumor cells, both in the GCB (p=0.003) andnon-GCB subtypes (p=0.000).ConclusionIn both subtypes, CD8 density is higher in peripheral than surrounding tumor cells and a high density in surrounding tumor cells willbe followed by an increase in peripheral CD8 density. ABSTRAKLatar belakangDiffuse Large B Cell Lymphoma (DLBCL) terbagi atas dua subtipe yaitu GCB dan non-GCB, yang ditentukan berdasarkan algoritmeHans, di mana subtipe GCB memiliki prognosis yang lebih baik dibanding non-GCB. Saat ini beberapa penelitian menunjukkanbahwa perkembangan tumor dapat dipengaruhi oleh lingkungan mikrotumor, di antaranya adalah TILs CD8+ yang berada di sekitarsel-sel tumor maupun di perifer. Penelitian ini bertujuan untuk mengetahui kepadatan TILs CD8+ pada lingkungan mikrotumor dikedua subtipe DLBCL.MetodeTelah dilakukan penelitian cross sectional pada kasus DLBCL yang di registrasi di Departemen Patologi Anatomik FakultasKedokteran Universitas Sriwijaya/RSUP Dr. Moh. Hoesin Palembang dari 1 Oktober 2017 sampai 31 Desember 2018, berjumlah 40sampel. Semua sampel dipulas menggunakan antibodi anti-CD8, kemudian dilakukan penilaian pada ekspresi CD8 pada sel-sellimfosit di sekitar sel-sel tumor dan di perifer. Kepadatan TILs CD8+ di kedua lokasi tersebut pada masing-masing subtipe DLBCLdianalisis uji Mann Whitney U TestHasilPada penelitian ini, non-GCB merupakan subtipe DLBCL terbanyak (72.5%) dan didominasi oleh usia muda yaitu 75% kasus.Secara signifikan, kepadatan TILs CD8+ di perifer lebih tinggi dibanding TILs CD8+ yang berada di sekitar sel-sel tumor, baik padasubtipe GCB (p=0.003) dan non-GCB (p=0.000).KesimpulanPada kedua subtipe, kepadatan TILs CD8+lebih tinggi di perifer dibanding di sekitar sel-sel tumor dan kepadatan yang tinggi disekitar sel-sel tumor akan diikuti peningkatan kepadatan CD8 di perifer Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/466 10.55816/mpi.v30i2.466 Majalah Patologi Indonesia; Vol. 30 No. 2 (2021): MPI; 227 - 233 2527-9106 0215-7284 10.55816/mpi.v30i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/466/328

oai:ojs.pkp.sfu.ca:article/467 2022-05-19T12:20:28Z patologi:ART driver

P53 Expression analysis in Low Grade and High Grade Urothelial Bladder Carcinoma and the Association with Tumor Stages Analisis Ekspresi P53 pada Karsinoma Urotelial Kandung Kemih Derajat Rendah dan Derajat Tinggi serta Hubungannya dengan Stadium Tumor Silitonga, Imelda Setiana Tanurahardja, Budiana Kusmardi urothelial carcinoma, bladder, p53, immunohistochemistry. karsinoma urotelial, kandung kemih, p53, imunohistokimia. BackgroundUrothelial carcinoma is the most common bladder malignancy in men. Risk factors include smoking, chemicals exposure, radiation,schistosoma hematobium infection. P53 mutation is the most common mutation in urothelial bladder carcinoma that cause p53 proteinaccumulation which are detected through immunohistochemical examination. The aim of this study is to analyze the differencesbetween p53 expression in low and high grade urothelial and the carcinomas association of p53 expression with tumor stage.MethodsThis was a cross sectional study with 47 cases (22 cases of low grade and 25 cases of high grade) urothelial carcinoma in AnatomicalPathology Department Faculty Medicine of Universitas Indonesia/Cipto Mangunkusumo Hospital (FKUI/RSCM) in 2009-2017. Allcases stained with p53 antibody with immunohistochemical methods with positive cut off ≥20%.ResultsP53 positive expression are found in 33 samples, 20 cases of high grade and 13 cases in low grade; 22 cases of Nonmuscle invasivebladder cancer and 11 cases of Muscle invasive bladder cancer.ConclusionThe expression of p53 in low grade and high grade urotelial carcinoma of the bladder and its association with tumor stage showed nosignificant difference. ABSTRAKLatar belakangKarsinoma urotelial merupakan keganasan kandung kemih tersering pada laki-laki. Faktor risikonya adalah merokok, pajanan bahankimia, radiasi, infeksi Schistosoma hematobium. Mutasi p53 merupakan mutasi tersering pada karsinoma urotelial kandung kemihyang menyebabkan akumulasi protein p53 di inti dan terlihat dengan imunohistokimia. Tujuan penelitian ini untuk menganalisisperbedaan ekspresi p53 pada karsinoma urotelial kandung kemih derajat rendah dan derajat tinggi serta hubungan ekspresi p53dengan stadium tumor.MetodePenelitian menggunakan desain potong lintang. Sampel terdiri atas 47 kasus yang terbagi menjadi 22 kasus karsinoma urotelialderajat rendah dan 25 kasus karsinoma urotelial derajat tinggi di Departemen Patologi Anatomik Fakultas Kedokteran UniversitasIndonesia/Rumah Sakit Cipto Mangunkusumo (FKUI/RSCM) tahun 2009-2017 dan dilakukan pulasan imunohistokimia p53 dengancut off positif ≥20%.HasilEkspresi p53 positif pada 33 sampel, 20 kasus karsinoma urotelial derajat tinggi, dan 13 kasus karsinoma urotelial derajat rendah; 22kasus Nonmuscle invasive bladder cancer dan 11 kasus Muscle invasive bladder cancer.KesimpulanEkspresi p53 pada karsinoma urotelial kandung kemih derajat rendah dan derajat tinggi serta hubungannya dengan stadium tumormenunjukkan perbedaaan tidak bermakna Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/467 10.55816/mpi.v30i2.467 Majalah Patologi Indonesia; Vol. 30 No. 2 (2021): MPI; 234 - 241 2527-9106 0215-7284 10.55816/mpi.v30i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/467/329

oai:ojs.pkp.sfu.ca:article/468 2022-05-19T12:20:47Z patologi:ART driver

Analyses of Fibroblast Activation Protein (FAP) Expression Rate to Clincopathology Factors and Chemotherapy Response in Ovarian Epithelial Malignant Tumors Analisis Ekspresi Fibroblast Activation Protein (FAP) terhadap Faktor Klinkopatologi dan Respon Kemoterapi pada Tumor Ganas Epitelial Ovarium Rinelda, Yunike Wresnindyatsih Farida, Aida Farida clinicopathology characteristic, epithelial ovarian carcinoma, FAP. karakteristik klinikopatologi, tumor ganas epitelial ovarium, Fibroblast Activation Protein BackgroundEpithelial ovarian carcinoma is the most common cause of death among gynecological malignancies in the women. The interactionamong cancer cells and tumor microenvironment plays roles in cancer proliferation and invasiveness. One of important component oftumor microenvironment that could stimulate tumor proliferation and invasiveness, is fibroblast. Activated fibroblasts also known ascarcinoma associated fibroblasts (CAFs). The purpose of research is to analyzed FAP expressions to clinicopathologic characteristic(histopathology subtype and clinical stadium of FIGO) and chemotherapeutic responses.MethodsThis study is a retrospective observational analytic study with cross-sectional designs was undertaken during January 1, 2016 to June30, 2018. The sample was 55 paraffin block sample epithelial ovarian carcinomas consists of 11 samples for each histopathologysubtype collected used purposive sampling technique. All samples were immunostained with FAP antibody. FAP expressions wasevaluated based on Min Shi et al studies. It analyzed stained area/ proportion (P) and intensity (I). Statistical analysis using chi-squarewas performed using SPSS version 17.ResultsPatients of 40-49 years old (24; 43.6%), clinical stadium is III (30; 54.5%), Complete response (CR) of chemotherapeutic response(38; 69.1%). The statistic analyses between histopathology subtypes (p=0.038), clinical stadium of FIGO (p=0.000) and chemotherapyresponse (p=0.000) showed significant differences.ConclusionFAP expressions could showed correlation between clinicopathologic characteristic (histopathology subtype and clinical stadium ofFIGO) and chemotherapeutic response. ABSTRAKLatar belakangTumor ganas epitelial ovarium adalah penyebab kematian tersering dari kanker ginekologi pada wanita. Interaksi antara sel-sel kankerdan lingkungan mikro tumor memainkan peran penting dalam proliferasi dan invasi sel-sel tumor. Salah satu komponen pentinglingkungan mikrotumor yang menstimulasi proliferasi dan invasi tumor, adalah fibroblas. Fibroblas dalam keadaan aktif (teraktivasi),disebut dengan cancer associated fibroblast (CAF). Tujuan penelitian untuk menganalisis ekspresi FAP terhadap karakteristikklinikopatologi (subtipe histopatologi dan stadium klinis FIGO) dan respon kemoterapi tumor ganas epitelial ovarium.MetodePenelitian ini merupakan penelitian observasional analitik dengan desain potong lintang dilakukan sejak 1 Januari 2016 sampai 30Juni 2018. Sampel sejumlah 55 sampel blok paraffin tumor ganas epitelial ovarium, terdiri atas 11 sampel untuk masing-masingsubtipe histopatologi, yang dikumpulkan menggunakan tehnik purposive sampling, dan dilakukan pemeriksaan immunohistokimiamenggunakan antibodi FAP. Ekspresi FAP dinilai berdasarkan penelitian Min Shi et al, yang menilai luas area/ proporsi (P) danintensitas (I) yang terpulas. Analisis statistik dilakukan menggunakan uji chi-square dan SPSS versi 17.HasilKelompok usia 40-49 tahun (24; 43.6%), stadium klinis III (30; 54.5%), respon kemoterapi complete response (38; 69.1%). Analisisstatistik antara subtipe histopatologi (p=0,038), stadium klinis FIGO (p=0,000), dan respon kemoterapi (p=0,000) menunjukkanhubungan yang bermakna.KesimpulanEkspresi FAP dapat menunjukkan hubungan antara karakteristik klinikopatologi (subtipe histopatologi dan stadium klinis FIGO) danrespon kemoterapi tumor ganas epitelial ovarium Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/468 10.55816/mpi.v30i2.468 Majalah Patologi Indonesia; Vol. 30 No. 2 (2021): MPI; 242 - 250 2527-9106 0215-7284 10.55816/mpi.v30i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/468/330

oai:ojs.pkp.sfu.ca:article/469 2022-05-19T12:21:20Z patologi:ART driver

Association of P16 Expression WITH Neoadjuvan Chemotherapy Histological Response in Conventional Osteosarcoma Hubungan antara Ekspresi P16 dengan Respon Histologik Kemoterapi Neoadjuvan pada Osteosarkoma Konvensional Vathonati, Dian Sari Oethia Siregar, Nurjati Chairani Wuyung, Puspita Eka conventional osteosarcoma, necrotic response to neoadjuvan chemotherapy, p16 Osteosarkoma konvensional, p16, respons histologik kemoterapi neoadjuvan BackgroundOsteosarcoma is the most common primary malignant bone tumor in children and young adult. Its pathogenesis has been linked toalterations in several genes. The high percentage is found involving Retinoblatoma (RB) pathway. p16 plays as a tumor suppressorin RB pathway to controll proliferation of the tumor cell. The degree of neoadjuvan chemotherapy histological necrosis response isrelated to prognosis of patients with osteosarcoma. Chemotherapy and p16 both synergic in inhibit the cell tumor proliferation andsupport apoptotic. Loss of p16 function is related to progressiveness of the tumor. The aim of this study was to investigate therelationship of p16 expression in pretreatment osteosarcoma to pathologic necrotic histological response after neoadjuvanchomotherapy.MethodsThis is a cross sectional study p16 stainning was done and count the positive expression tumor cell in percentage. Positive wasdefined as strong and medium nuclear stainning in 30% or greater. The samples is catagorized into positive and negative expressionthen it is correlated into tumor necrotic area based on grade of Huvos.ResultsSamples consist of 33 cases. Positive stainning was found in 10 cases (30.3%), 6 of 10 cases had good chemotherapy response.Negative stainning was found in 23 cases and 21 of 23 cases had poor chemotherapy response. A significant association was notedbetween p16 expression and histological necrotic response to neoadjuvan chemotherapy (p=0.004).ConclusionThe result showed that p16 expression associate significantly with histological necrotic response to neoadjuvan chemotherapy inconventional osteosarcoma (p=0.004). ABSTRAKLatar belakangOsteosarkoma merupakan tumor ganas primer tulang yang paling banyak ditemukan pada anak dan dewasa muda. Patogenesisnyamelibatkan berbagai perubahan gen yang kompleks. Jalur utama yang berperan dalam patogenesisnya antara lain melalui jalurprotein Retinoblastoma (RB). p16 bekerja sebagai tumor suppressor pada jalur RB dalam menghambat pembelahan sel tumor. Salahsatu prognosis osteosarkoma dipengaruhi oleh respon histologik kemoterapi neoadjuvan. Kemoterapi dan p16 keduanya salingbekerja sama di dalam menghambat pembelahan sel dan memicu apoptosis. Hilangnya fungsi p16 berkaitan dengan progresivitassel tumor. Tujuan penelitian ini untuk mengetahui hubungan ekspresi p16 dengan respon kemoterapi neoadjuvan pada osteosarkomakonvensional.MetodePenelitian ini menggunakan desain potong lintang. Dilakukan pulasan imunohistokimia p16 pada setiap kasus biopsi sebelumkemoterapi neoadjuvan, kemudian dihitung persentase sel tumor yang positif. Ekspresi dikategorikan positif apabila inti sel tumorterpulas sedang atau kuat pada >30% sel tumor. Hasil perhitungan dikelompokkan menjadi ekspresi positif dan negatif kemudiandikorelasikan dengan luas nekrosis reseksi tumor setelah kemoterapi neoadjuvan.HasilDiperoleh 33 kasus osteosarkoma konvensional yang sesuai dengan kriteria inklusi. Ekspresi positif ditemukan 10 kasus (30,3%) danekspresi negatif 23 kasus (69,7%). Pada ekspresi positif, 6 dari 10 kasus memiliki respon kemoterapi baik dan pada ekspresi negatif,21 dari 23 kasus memiliki respon kemoterapi buruk. Hasil penelitian menunjukkan ekspresi imunohistokimia p16 berhubungansignifikan dengan respon histologik baik kemoterapi neoadjuvan (p=0,004).KesimpulanEkspresi p16 positif memiliki hubungan yang sejalan dengan keberhasilan respon histologik kemoterapi neoadjuvan padaosteosarkoma konvensional. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/469 10.55816/mpi.v30i2.469 Majalah Patologi Indonesia; Vol. 30 No. 2 (2021): MPI; 251 - 258 2527-9106 0215-7284 10.55816/mpi.v30i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/469/331

oai:http://majalahpatologiindonesia.com/p/index.php/patologi/oai:article/470 2021-05-24T05:34:24Z patologi:ART driver

oai:ojs.pkp.sfu.ca:article/471 2022-05-19T12:22:13Z patologi:ART driver

Relationship between Tumor-Associated-Macrophages and Tumor-Associated-Neutrophils Density with Clinicohistopathological Characteristics of Hodgkin's Lymphoma Patients Hubungan Kepadatan Tumor-Associated-Macrophages dan TumorAssociated-Neutrophils dengan Karakteristik Klinikohistopatologi pada Pasien Limfoma Hodgkin Putri, Nadya Virana Neti Puspita Rasyid, Riana Sari Murti, Krisna Hodgkin lymphoma, TAM, TAN, tumor microenvironment, clinicohistopathology. limfoma Hodgkin, TAM, TAN, lingkungan mikro tumor, klinikohistopatologi. BackgroundThe development and aggressiveness of tumor cells were influenced by tumor microenvironment. Tumor-Associated-Macrophages(TAMs) and Tumor-Associated-Neutrophils (TANs) are components of tumor microenvironment, contribute to development and growthof tumor cells. The aim of this research was to analyse correlation of TAMs and TANs densities with clinicohistopathologiccharacteristics of patients i.e. age, gender, location, and subtype.MethodsA case series was conducted. The samples were paraffin blocks of Hodgkin lymphoma patients at Anatomic Pathology DepartmentFaculty of Medicine Universitas Sriwijaya/RSUP Dr. Mohammad Hoesin Palembang. Among 44 cases of Hodgkin lymphomadiagnosed from 1stJanuari 2015 to 30th October 2019, only 31 cases were included. Immunohistochemistry was performed by usinganti-CD163 antibody and neutrophils were identified by morphologic appearances. The results were correlated to characteristicfactors. Statistical analysis was performed using Spearman and Lambda test with significant consideration if p<0.05.ResultsThe study results showed 30 cases of classical Hodgkin lymphoma (CHL) and 1 case of NLPHL. CHL has only 3 variants LR(lymphocyte-rich) (60%), MC (mixed-cellularity) (26.7%), and NS (nodular sclerosis) (13.3%). Hodgkin lymphoma is most commonlyfound in males (61.3%). Tumor were mostly found in the head and neck, and patient ages were majority in the age group 20-29 years,40-49 years, and over 50 years. Spearman test showed there was a significant correlation between density of TAMs with ages(p=0.043).ConclusionHigh density of TAM was correlated with age, however, there was no correlation between TAN and clinicohistopathologiccharacteristics of Hodgkin lymphoma. ABSTRAKLatar belakangProses perkembangan sel-sel tumor pada limfoma Hodgkin dikaitkan dengan peranan lingkungan mikro tumor. Tumor-AssociatedMacrophages (TAMs) dan Tumor-Associated-Neutrophils (TANs) merupakan komponen lingkungan mikro yang mempengaruhipertumbuhan dan perkembangan tumor. Kepadatan TAMs dan TANs tinggi mempengaruhi perjalanan penyakit pasien. Tujuanpenelitian ini adalah melihat hubungan kepadatan TAMs dan TANs terhadap karakteristik klinikohistopatologi pasien, yaitu usia, jeniskelamin, lokasi, dan subtipe.MetodePenelitian ini menggunakan metode case series. Sampel penelitian ini yaitu pasien limfoma Hodgkin di Departemen Patologi AnatomikRSUP Dr. Mohammad Hoesin Palembang. Didapatkan 44 kasus limfoma Hodgkin selama periode 1 Januari 2015-30 Oktober 2019,dan 31 sampel yang memenuhi kriteria inklusi. Dilakukan pemeriksaan immunohistokimia menggunakan antibodi spesifik untukmakrofag (anti-CD163) dan identifikasi neutrofil dilakukan dengan melihat morfologinya. Analisis statistik menggunakan uji Spearmandari SPSS versi 23.0, dianggap berhubungan bila p<0,05.HasilSebanyak 31 sampel terdiri dari 30 sampel (96,8%) merupakan limfoma Hodgkin tipe klasik dan 1 sampel merupakan NLPHL (3,2%).Sebaran kasus CHL masing-masing subtipenya yaitu LR (lymphocyte-rich) (60%), MC (mixed-cellularity) (26,7%), dan NS (nodularsclerosis) (13,3%). Limfoma Hodgkin paling banyak ditemukan pada laki-laki, lokasi tumor terbanyak di regio kepala-leher, serta palingbanyak pada kelompok usia 20-29 tahun, 40-49 tahun, dan 50 tahun. Terdapat hubungan kepadatan TAMs dengan usia (p=0,043).KesimpulanTerdapat hubungan antara kepadatan TAMs dengan karakteristik klinikohistopatologi yaitu usia, namun tidak terdapat hubunganantara kepadatan TANs dengan karakteristik klinikohistopatologi limfoma Hodgkin. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/471 10.55816/mpi.v30i2.471 Majalah Patologi Indonesia; Vol. 30 No. 2 (2021): MPI; 264 - 271 2527-9106 0215-7284 10.55816/mpi.v30i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/471/333

oai:ojs.pkp.sfu.ca:article/473 2022-05-19T12:23:03Z patologi:ART driver

The Correlation of BRAF V600E Expressions with Histopathological Variant and Lymphocyte Infiltration in Papillary Thyroid Carcinoma Hubungan Ekspresi BRAF V600E dengan Varian Histopatologik dan Infiltrasi Limfosit pada Papillary Thyroid Carcinoma (PTC) Gestin, Dutia RZ Nizar Asri, Aswiyanti Yetti, Husna papillary thyroid carcinoma, BRAF V600E expression, histopathological variant, lymphocyte infiltration papillary thyroid carcinoma, ekspresi BRAF V600E, varian histopatologik, infiltrasi limfosit. BackgroundPapillary thyroid carcinoma is the most common endocrine gland malignancy that has the highest incidence rate of 60-80%. In papillarythyroid carcinoma BRAF V600E mutation plays an important role in carcinogenesis. BRAF V600E is a RAS-regulated-serine-threoninekinase and activator of the MAPK pathway cascade that functions to regulate cell proliferation, differentiation and survival. BRAFV600E mutations in PTC is associated with a high incidence of recurrence and reduced survival. The purpose of this study is to lookat the relationship of BRAF V600E expression with histopathological variants and lymphocyte infiltration in PTC.MethodsThis research was a retrospective observational cross sectional study with 40 cases of papillary thyroid carcinoma which werediagnosed in 4 Pathology Anatomy Laboratory in West Sumatera. Samples were obtained from paraffin blocks of tissue from surgeryand were reevaluated for histopathological variant and lymphocyte infiltration. Expression of BRAF V600E in tumor cell were analyzedusing immunohistochemistry staining. Bivariate statistical analysis of Chi-Square were used and p value <0,05 were consideredsignificance.ResultsPositive BRAF V600E expression was found in 24 case (60%). Positive BRAF V600E expression was mostly found in classic PTC(50%). Papillary thyroid carcinoma with lymphocyte infiltration mostly found with negative BRAF V600E expression (79.2%). Statisticalanalysis showed no significant correlation between BRAF V600E with histopathological variant (p=0.74) and no significancecorrelation between BRAF V600E expression with lymphocyte infiltration (p=0.12).ConclusionThe conclusion is BRAF V600E expression has no correlation with histological variants in papillary thyroid carcinoma and no significantcorrelation of BRAF V600E expression with lymphocyte infiltration. ABSTRAKLatar belakangPapillary thyroid carcinoma (PTC) merupakan keganasan kelenjar endokrin yang paling sering ditemukan, memiliki angka kejadianpaling tinggi yaitu sekitar 60-80%. Pada papillary thyroid carcinoma mutasi BRAF V600E berperan penting pada karsinogenesis.BRAF V600E merupakan suatu RAS-regulated-serin-threonine kinase dan aktivator kaskade jalur MAPK, yang berfungsi mengaturproliferasi, differensiasi dan survival sel. Mutasi BRAF V600E pada PTC dikaitkan dengan tingginya kejadian rekurensi danmengurangi kelangsungan hidup. Tujuan penelitian ini untuk melihat hubungan ekspresi BRAF V600E dengan varian histopatologikdan infiltrasi limfosit pada papillary thyroidcarcinoma.MetodePenelitian ini merupakan penelitian observasional dengan desain cross-sectional dengan sampel penelitian sebanyak 40 kasus PTCyang telah didiagnosis di 4 Laboratorium Patologi Anatomik di Sumatera Barat. Sampel diperoleh dari blok paraffin yang berasal darijaringan hasil operasi dan dilakukan reevaluasi terhadap varian histopatologik dan infiltrasi limfosit. Ekspresi BRAF V600E pada seltumor dilihat dengan pewarnaan imunohistokimia. Analisis statistik bivariat menggunakan uji chisquare dengan p<0,05 dianggapbermakna.HasilEkspresi BRAF V600E positif ditemukan pada 24 kasus (60%). Ekspresi BRAF V600E positif paling banyak ditemukan pada kasusclassic PTC (50%). Kasus PTC dengan infiltrasi limfosit paling banyak ditemukan dengan ekspresi BRAF V600E negatif (79,2%).Analisis statistik menunjukkan tidak terdapat hubungan bermakna antara ekspresi BRAF V600E dengan varian histopatologik (p=0,74)dan tidak terdapat hubungan bermakna antara ekspresi BRAF V600E dengan infiltrasi limfosit (p=0,12).KesimpulanPada penelitian ini tidak terdapat hubungan bermakna antara ekspresi BRAF V600E dengan varian histopatologi dan tidak terdapathubungan yang bermakna antara ekspresi BRAF V600E dengan infiltrasi limfosit Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/473 10.55816/mpi.v30i2.473 Majalah Patologi Indonesia; Vol. 30 No. 2 (2021): MPI; 272 - 279 2527-9106 0215-7284 10.55816/mpi.v30i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/473/334

oai:ojs.pkp.sfu.ca:article/476 2022-05-19T12:22:35Z patologi:ART driver

The Role of Memory T Cells in Controlling the Covid-19 Pandemic Peran Sel T Memori dalam Pengendalian Pandemi Covid-19 Kusmardi Ramadhian N, Dimas Pratomo, Irandi P Tedjo, Aryo Sars-Cov-2; vaccinations; antibody; memory T cells; cell-mediated immunity Sars-Cov-2; vaksinasi; antibodi; sel T memori; cell-medited immunity ABSTRACTEvaluation of changes in antibody orders is a common exclusion in vaccination strategies because of the method ofanalysis. The fact that protection by antibodies produced by the Severe Acute Respiratory Syndrome Coronavirus-2(Sars-Cov-2) infection both naturally and through vaccines will decrease in less than one year, is a challenge for acountry with a large population like Indonesia to run its vaccination program. The main challenge is whether thevaccination strategy adopted will overcome the vaccine barrier and the race between the rate of vaccination and therate of viral mutation and antibody reduction. In addition to antibodies, the adaptive immune system is also run by Tcells that are included in the cell-mediated immune system (CMI) group. In patients with asymptomatic or mildsymptoms of Coronavirus 2019 (Covid-19), T and CMI responses are known to appear in some patients who are notknown to have been exposed to Sars-Cov-2 before. This evidence suggests that the adaptive catastrophe for SarsCov-2 has been acquired by ordering memory T cells and may last longer than previously thought. For countries withlarge populations, this will certainly help overcome the limitations of vaccines and the time needed to implement theirvaccination strategies. Evaluasi berdasarkan pembentukan antibodi merupakan hal umum yang dipertimbangkan dalam strategi vaksinasi karena kemudahan metode analisisnya. Fakta bahwa perlindungan oleh antibodi yang dihasilkan oleh infeksi Severe acute respiratory syndrome coronavirus-2 (Sars-Cov-2) baik secara alami maupun melalui vaksin akan mengalami penurunan dalam waktu kurang dari satu tahun, menjadi tantangan bagi negara dengan jumlah penduduk besar seperti Indonesia untuk menjalankan program vaksinasinya. Tantangan utamanya adalah apakah strategi vaksinasi yang diambil akan berhasil melewati hambatan ketersediaan vaksin dan perlombaan antara laju vaksinasi dengan laju mutasi virus dan penurunan antibodi. Selain antibodi, sistem imun adaptif juga dijalankan oleh sel T yang masuk dalam kelompok sistem kekebalan diperantarai sel atau cell-medited immunity (CMI). Pada pasien Coronavirus disease 2019 (Covid-19) asimtomatik atau gejala ringan, respon sel T dan CMI diketahui muncul pada beberapa pasien yang tidak diketahui pernah terpapar oleh Sars-Cov-2 sebelumnya. Bukti ini menunjukkan bahwa kekebalan adaptif terhadap Sars-Cov-2 telah diperoleh melalui pembentukan sel T memori dan mungkin dapat berlangsung lebih lama dari yang selama ini diperkirakan. Bagi negara-negara dengan jumlah penduduk besar, hal tersebut tentunya akan membantu mengatasi keterbatasan vaksin dan waktu yang dibutuhkan untuk menjalankan strategi vaksinasinya. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-04-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/476 10.55816/mpi.v30i2.476 Majalah Patologi Indonesia; Vol. 30 No. 2 (2021): MPI 2527-9106 0215-7284 10.55816/mpi.v30i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/476/335

oai:ojs.pkp.sfu.ca:article/477 2025-12-19T17:09:15Z patologi:ART driver

Analysis of Clinicopathological Characteristics in Luminal A like subtype Breast Carcinoma Analisis Karakter Klinikopatologik pada Kasus Karsinoma Payudara Subtipe Luminal A Like Susanto, Andi Dewayani,, Birgitta Maria Hernowo, Bethy Surjawathy breast carcinoma, high histoscore, low histoscore, luminal A like karsinoma payudara, histoskor tinggi, histoskor rendah, luminal A like BackgroundMolecular subtypes of breast carcinoma, luminal A like has the best prognosis, but in the previous studies there were cases thatshowed poor clinical outcomes. This study attempted to investigate the clinicopathological characteristics in luminal A like subtypebreast carcinoma.MethodsThis study is a cross sectional study. The samples used were 67 medical records of patients with luminal A like subtype breastcarcinoma in January 2014 to June 2019 period from Hasan Sadikin Hospital, Bandung. According to estrogen receptor (ER) andprogesteron receptor (PR) expression status by adding both intensity and distribution scores, the data were divided into twosubgroups: low histoscore (<7) and high histoscore (≥7). Clinicopathological characteristics (age, TNM stage, treatment, location,histologic type, grading and metastasis) were evaluated for each subgroup.ResultsFrom 67 luminal A like subtype breast carcinoma patients, 73.13% were in the high histoscore subgroup, with higher percentage oflow grade histopathologic features (63.27%). There were no significant differences in age and TNM staging between the twosubgroups. Among 67 breast carcinoma patients, 43 were diagnosed in advanced stages. There were significant differences inlymph nodes metastasis between the two subgroups (p-value 0.013), however, there were no significant difference in metastasis toother organs.ConclusionThis study shows that 73.13% of patients with luminal A like subtype breast carcinoma have a high histoscore of hormone receptor.Low histoscore subgroups luminal A like subtype breast carcinoma will metastasize to lymph nodes. Latar belakangKarsinimo payudara subtipe molekular luminal A like mempunyai prognosis yang paling baik, namun pada penelitian sebelumnyaterdapat kasus yang menunjukkan kondisi klinik yang buruk. Penelitian ini bertujuan untuk mengetahui karakter klinikopatologikkarsinoma payudara subtipe luminal A like.MetodePenelitian ini merupakan penelitian potong lintang. Sampel yang digunakan adalah data rekam medik 67 pasien karsinomapayudara subtipe luminal A like periode Januari 2014 sampai Juni 2019 di Rumah Sakit Hasan Sadikin, Bandung. Berdasarkanpenjumlahan skor intensitas dan distribusi imunoekspresi reseptor estrogen (ER) dan reseptor progesteron (PR) pada inti sel tumor,data dibagi menjadi 2 kelompok, yaitu kelompok histoskor rendah (<7) dan histoskor tinggi (≥7). Karakter klinikopatologik (usia,stadium: tumour-node-metastasis/TNM, terapi, lokasi, tipe histopatologik, derajat histopatologik dan metastasis) dinilai untukmasing-masing kelompok.HasilPenelitian ini menunjukkan bahwa dari total 67 pasien karsinoma payudara subtipe luminal A like, 73,13% termasuk dalamkelompok histoskor tinggi dengan persentase gambaran histopatologik derajat rendah yang lebih tinggi (63,27%). Tidak terdapatperbedaan yang bermakna pada usia dan stadium TNM antara kelompok histoskor rendah dan histoskor tinggi. Dari total 67 pasienkarsinoma payudara, 43 pasien terdiagnosis pada stadium lanjut. Terdapat perbedaan yang bermakna dalam kejadian metastasiske kelenjar getah bening antara kelompok histoskor rendah dan histoskor tinggi (p=0,013), namun tidak terdapat perbedaan yangbermakna dalam metastasis ke organ lainnya.KesimpulanSebagian besar pasien karsinoma payudara subtipe luminal A like memiliki nilai histoskor reseptor hormon yang tinggi (73,13%).Karsinoma payudara subtipe luminal A like kelompok histoskor rendah akan bermetastasis ke kelenjar getah bening. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/477 10.55816/mpi.v30i3.477 Majalah Patologi Indonesia; Vol. 30 No. 3, September 2021; 280 - 285 2527-9106 0215-7284 10.55816/mpi.v30i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/477/336

oai:ojs.pkp.sfu.ca:article/478 2025-12-19T17:09:15Z patologi:ART driver

The Relationship of Fibroblast Activation Protein (FAP) Expression to Clinicopathologic Factors of Papillary Thyroid Carcinoma Hubungan Tingkat Ekspresi Fibroblast Activation Protein (FAP) Terhadap Faktor Klinikopatologi Karsinoma Tiroid Papiler Herlina Maulani, Heni Apriyani, Nursanti clinicopathology characteristic, papillary thyroid carcinoma, FAP Karakteristik klinikopatologi, karsinoma tiroid papiler, FAP. BackgroundPapillary thyroid carcinoma is the most common subtype among thyroid cancers. The interaction between cancer cells and tumormicroenvironment plays roles in cancer proliferation and invasiveness. One of important components of tumor microenvironmentthat could stimulate tumor proliferation and invasiveness is activated fibroblast, known as carcinoma associated fibroblasts (CAFs).The aim of this research is to analysed FAP expression is associated with clinicopathologic characteristics.MethodsThis retrospective descriptive observational study with serial cases design was conducted in 40 samples of the paraffin blocks oftissues registered in Department of Anantomical Pathology Faculty of Medicine University of Sriwijaya/Dr. Moh. Hoesin PalembangHospital from January 1st, 2017 to October 31st, 2019. All samples were immunostained with FAP antibody. The expressions of FAPwere evaluated in accordance with the results of the previous study. Statistical test was performed by using Chi-square andSpearman-rho statistical test. Statistical analysis was performed by using SPSS version 23.0.ResultsOur data indicated that patients who were ≥45 years old (21 cases; 52.5%), women (31 cases; 77.5%) suffered from this cancer themost. The stromal type found in ten classic, follicular, solid and columnar variance were pauci (25 cases; 62.6%), desmoplastic (8cases;20%), sclerosis (5 cases; 12.5%) and inflammatory (2 cases; 5%). The expression of FAP in tumor stroma has a significantcorrelation with histopathology subtypes (p=0.028) and stromal type (p=0.000).ConclusionFAP was expressed in tumor epithelial cells and fibroblast stromal tumour of papillary thyroid carcinoma. FAP expression in stromaltumor between histopathologic subtype and stromal type showed significant correlation Latar belakangKarsinoma tiroid papiler adalah tipe histologi karsinoma tiroid paling banyak angka kejadiannya dibandingkan karsinoma tiroidlainnya. Interaksi antara sel-sel tumor ganas dan lingkungan mikro tumor berperan penting dalam proliferasi dan invasi sel-seltumor. Salah satu komponen penting lingkungan mikro tumor yang menstimulasi proliferasi dan invasi tumor adalah fibroblasteraktivasi cancer associated fibroblast (CAF). Tujuan penelitian ini untuk menganalisishubungan tingkat ekspresi FAP terhadapfaktor klinikopatologi karsinoma tiroid papiler.MetodePenelitian ini merupakan penelitian deskriptif observasional dengan desain serial kasus terhadap blok parafin jaringan yangterdaftar di Departemen Patologi Anatomik Fakultas Kedokteran Universitas Sriwijaya/RS. Dr. Moh. Hoesin Palembang sejak 1Januari 2017 sampai 31 Oktober 2019. terhadap 40 sampel dilakukan pemeriksaan immunohistokimia menggunakan antibodi FAP.Ekspresi FAP dinilai berdasarkan penelitian sebelumnya. Uji statistik menggunakan uji statistik Chi-Square dan Spearman-rho.Analisis statistik menggunakan SPSS versi 23.0.HasilKarsinoma tiroid papiler paling banyak ditemukan pada kelompok usia ≥45 tahun (21; 52,5%), jenis kelamin perempuan (31;77,5%). Stroma tumor terbanyak ditemukan diantara masing-masing 10 varian klasik, folikular, kolumnar dan solid adalah stromapauci (25; 62,5%), desmoplastik (8; 20%), sklerosis (5; 12,5%) dan inflamatori (2; 5%). Terdapat hubungan yang bermakna antaratingkat ekspresi FAP pada stroma dengan subtipe histopatologi (p=0,028) dan jenis stroma (p=0,000).KesimpulanFAP terekspresi pada sel-sel epitel tumor dan fibroblas stroma karsinoma tiroid papiler. Ekspresi FAP pada stroma tumor antarasubtipe histopatologi dan jenis stroma menunjukkan hubungan yang bermakna Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/478 10.55816/mpi.v30i3.478 Majalah Patologi Indonesia; Vol. 30 No. 3, September 2021; 286 - 294 2527-9106 0215-7284 10.55816/mpi.v30i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/478/337

oai:ojs.pkp.sfu.ca:article/479 2025-12-19T17:09:15Z patologi:ART driver

CK19 and PDX-1 Immunohistochemistry: Accuracy on Detecting Pancreatic Tumor and Its Metastases Ketepatan Pemeriksaan Imunohistokimia CK19 Dibandingkan dengan PDX-1 pada Tumor Pankreas dan Anak Sebarnya Rosari, Bayu Perkasa Linggodigdo, Michelle Wijaya, Yan Cahya Stephanie, Marini Nur Rahadiani pancreatic tumor, pancreatic tumor metastasis, immunohistochemistry, PDX-1, CK19. tumor pankreas, metastasis tumor pankreas, imunohistokimia, PDX-1, CK19 BackgroundMetastatic adenocarcinoma of unknown origin remains a serious problem in oncology. Pancreatic cancer is often diagnosed when ithas already metastasized. Therefore immunohistochemistry (IHC) examination for detecting metastases of pancreatic origin is veryimportant IHC marker of Pancreatic duodenal homeobox-1 (PDX-1) has a good accuracy for detecting metastases of pancreaticorigin, however it is not readily available in many centers in Indonesia. Instead, IHC marker that is often being used is CK19. Thismarker is not specific, because it is also expressed by gastrointestinal and hepatobilliary tumors. This evidence-based case reportaims to determine the accuracy of CK19 compared with PDX-1 IHC for detecting pancreatic tumor and its metastases.MethodsLiterature searches were conducted on Pubmed/MEDLINE®, Scopus®, Cochrane®databases. Four articles about PDX-1 and 5 articlesabout CK19 that are relevant were found. Those articles were critically appraised.ResultsAll articles are considered valid, as they fulfilled the important criteria for journal validity. A study of CK19 IHC found that this markerhas 100% sensitivity and 6% specificity for diagnosing liver metastases from well-differentiated pancreatic ductal adenocarcinoma.Studies of PDX-1 IHC have different results; a study found that 27 out of 67 were PDX-1 positive in metastases of pancreaticadenocarcinoma origin, while another study found that PDX-1 has 72% sensitivity and 93% specificity for pancreatic neuroendocrinetumor, with 100% sensitivity and 75% specificity for pancreatic neuroendocrine tumor metastases.ConclusionCK19 IHC is highly sensitive for detecting adenocarcinoma metastases of pancreatic origin, thus it can be utilized as a screening test.However, CK19 IHC specificity for detecting adenocarcinoma metastases of pancreatic origin is low compared to PDX-1 IHC Latar belakangMetastasis adenokarsinoma yang tidak diketahui asalnya merupakan permasalahan onkologi yang serius. Salah satu tumor yangsering ditemukan ketika sudah bermetastasis adalah kanker pankreas. Pemeriksaan untuk mendeteksi anak sebar asal tumor primerpankreas sangatlah penting, salah satunya adalah pulasan imunohistokimia (IHK). Penanda IHK Pancreatic duodenal homeobox-1(PDX-1) memiliki akurasi yang baik untuk mendeteksi anak sebar pankreas, namun penanda ini belum tersedia di banyak senter diIndonesia. Penanda yang sering digunakan adalah CK19, namun pemeriksaan ini kurang spesifik, oleh karena diekspresikan jugaoleh tumor saluran cerna dan hepatobilier. Laporan kasus berbasis bukti ini bertujuan menilai ketepatan pemeriksaan IHK CK19dibandingkan PDX-1 pada kasus tumor pankreas dan anak sebarnya.MetodeDilakukan pencarian literatur pada basis data Pubmed/MEDLINE®, Scopus®, Cochrane®. Didapatkan 4 artikel mengenai PDX-1 dan 5artikel mengenai CK19 yang relevan, kemudian dilakukan telaah kritis.HasilSemua artikel dinilai valid karena memenuhi kriteria validitas artikel yang penting. Studi IHK CK19 menyatakan pemeriksaan inimemiliki sensitifitas 100% dan spesifisitas 6% untuk adenokarsinoma duktal pankreas berdiferensiasi baik yang bermetastasis ke hati.Studi IHK PDX-1 hasilnya bervariasi; satu studi menemukan PDX-1 positif pada 27 dari 67 kasus metastasis adenokarsinomapankreas, studi lainnya menemukan PDX-1 memiliki sensitifitas 72% dan spesifisitas 93% untuk tumor primer neuroendokrinpankreas, dengan sensitifitas 100% dan spesifisitas 75% untuk metastasis tumor neuroendokrin pankreas.KesimpulanPemeriksaan CK19 memiliki sensitifitas tinggi untuk mendeteksi anak sebar adenokarsinoma dari pankreas, sehingga dapatdigunakan sebagai tes skrining. Namun, spesifisitas IHK CK19 untuk mendeteksi anak sebar adenokarsinoma pankreas lebih rendahdibanding dengan IHK PDX-1. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/479 10.55816/mpi.v30i3.479 Majalah Patologi Indonesia; Vol. 30 No. 3, September 2021; 295 - 303 2527-9106 0215-7284 10.55816/mpi.v30i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/479/338

oai:ojs.pkp.sfu.ca:article/480 2025-12-19T17:09:15Z patologi:ART driver

PD-L1 and TILS Expression among Triple Negative and HER2 Enriched Molecular Subtype of Breast Carcinoma Perbandingan Ekspresi PD-L1 dan TILS pada Karsinoma Payudara Triple Negative dan HER2 Enriched Asprianto, Muhammad Murti, Krisna Dewi, Citra Bahar, Erial PD-L1 and TILs expression, subtype triple negative, HER2 enriched. ekspresi PD-L1 dan TILs, subtype triple negative, HER2 enriched BackgroundTriple negative and positive HER 2 subtype breast carcinoma are aggressive subtype that have worst prognosis among othersubtype. High level of tumor infiltrating lymphocytes (TILs) is associated with longer disease free survival and overall survival.Breast cancer is also influenced by the presence of ligation between PD-1 and PD-L1, expressed by activated CD8+ T cells andtumor cells, consecutively. To analysed the comparison of PD-L1 expression levels in triple negative breast cancer and HER2enriched subtype.MethodsA cross sectional study was conducted with 35 samples, which were immunostained with an anti-PD-L1 antibody. TILs weremeasured on HE slides, and together with PD-L1 expression were calculated using image J. Clinicopathological data were obtainedfrom medical record. All data were analysed by SPSS V25.0ResultsA significant difference was observed between PD-L1 expression in HER2 enriched and triple negative samples, where the HER2enriched subtype had a possibility to express PD-L1 3.3 times higher than those of triple negative (p = 0.021; OR = 3.3). There wereno significant differences in clinopathological characteristics or TILs density in both subtypes. Likewise, no significant differenceswas found among PD-L1 expression, and intratumoral and stromal TILs expression.ConclusionMajority patient of both subtypes are mostly older than 40 years old, with positive LVI and higher histological grading. The HER2expression showed a higher level of PD-L1 than triple negative. There was no significant difference of TILs expression betweenthese two subtypes of breast cancer. Latar belakangKarsinoma payudara triple negative dan HER2 enriched merupakan subtipe agresif dengan prognosis paling buruk diantara subtipekarsinoma payudara lainnya. Karsinoma payudara dengan kepadatan tinggi (TILs) sering berhubungan dengan DFS (Disease FreeSurvival) dan OS (Overall Survival) lebih lama. Prognosis kanker payudara juga dipengaruhi oleh adanya ligasi antara ProgrammedCells Death-1 (PD-1) dan Programmed Cells Death-Ligands 1 (PD-L1) yang diekspresikan oleh sel T CD8+ dan sel tumor. Tujuanpenelitian ini untuk mengetahui perbandingan kepadatan PD-L1 dan TILs pada karsinoma payudara subtipe molekuler triplenegative dan HER2 enriched.MetodePenelitian cross sectional design dengan 35 sampel dipulas anti-PD-L1 (clone SP142; Abcam). TILs dihitung pada slaid pulasanHematoxylin-Eosin (HE), bersama dengan penghitungan ekspresi PD-L1 menggunakan Image J. Data klinikopatologi didapat darirekam medis. Seluruh data dianalisis menggunakan SPSS versi 25.0HasilTerdapat perbedaan bermakna kepadatan PD-L1, subtipe HER2 enriched memiliki kepadatan PD-L1 3,3 kali lebih besar dibandingtriple negative (p=0,021;OR=3,3). Terdapat perbedaan tidak bermakna dari kepadatan TILs pada kedua subtipe terhadapkarakteristik klinikopatologi (p>0,05). Terdapat korelasi sangat lemah antara PD-L1 dan TILs.KesimpulanSubtipe triple negative dan HER2 enriched banyak dijumpai pada kelompok usia di atas 40 tahun dengan ILV positif dan gradinghistopatologi tinggi. Ekspresi PD-L1 dan TILs dapat digunakan untuk mendeteksi karsinoma payudara subtipe triple negative danHER2 enriched. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/480 10.55816/mpi.v30i3.480 Majalah Patologi Indonesia; Vol. 30 No. 3, September 2021; 304 - 313 2527-9106 0215-7284 10.55816/mpi.v30i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/480/339

oai:ojs.pkp.sfu.ca:article/481 2025-12-19T17:09:15Z patologi:ART driver

Correlation of PD-L1 Expression with TILs CD8+ in Non-small Cell Carcinoma Lung Cancer Korelasi Ekspresi PD-L1 dengan TILs CD8+ pada Kanker Paru Jenis Karsinoma Bukan Sel Kecil Utami, Erisca Ayu Ika Kartika, Ika Kartika Rusminan, Suly Auline Erial Bahar, Erial Bahar Krisna Murti, Krisna Murti expression of PD-L1, non small cell lung cancer, density of CD8 TILs. ekspresi PD-L1, kanker paru jenis karsinoma bukan sel kecil, kepadatan TILs CD8 BackgroundNon-small cell lung cancer (NSCLC) is an epithelial carcinoma of the lung which consists of adenocarcinoma, squamous cellcarcinoma (SCC), and large-cell carcinoma (LCC). In a microtumor environment, PD-L1 and PD-1 bonds are immune checkpointsthat act in suppressing the activity of CD8+ TILs, thus affecting the prognosis of NSCLC. Aims of this study is to determine thecorrelation between PD-L1 expression with CD8+TILs in NSCLC.MethodsThis was a cross-sectional study of NSCLC registered in Department of Anatomical Pathology Faculty of Medicine University ofSriwijaya/Dr. Moh. Hoesin Palembang Hospital since 1 January 2017 to 31 January 2020 with a total of 24 samples. All sampleswere immunostained using PD-L1 and CD8 antibodies. PD-L1 expression was assessed in tumor mass and CD8 expression inintratumoral and peritumoral TILs.ResultsOnly two subtypes, adenocarcinoma and SCC, were found in this study. Adenocarcinoma was the most frequent subtype (54.1%),sample group is dominated by <60 years old and was the subtype that expressed the PD-L1 most strongly (TPS ≥50%). There wasnot significant correlation between PD-L1 expression with CD8+ TILs in NSCLC (p=0.679 and p=0.826) but based onclinicopathological characteristics, there was a significant relationship between PD-L1 with disease stage and between intratumoralCD8+ TILs density with disease stage (p=0.048 and p=0.017).ConclusionAdenocarcinoma subtype had stronger expression of PD-L1 compared to SCC. There was no correlation between PD-L1 and CD8+TILs density in NSCLC. Latar belakangKanker paru jenis karsinoma bukan sel kecil merupakan kelompok kanker paru epitelial yang terdiri dari adenokarsinoma,karsinoma sel skuamosa (KSS) dan karsinoma sel besar (KSB). Di dalam lingkungan mikrotumor, ikatan PD-L1 dan PD-1merupakan unsur immune checkpoint berperan dalam menekan aktivitas TILs CD8+sehingga mempengaruhi prognosis KPKBSK.Penelitian ini bertujuan untuk mengetahui korelasi ekspresi PD-L1 dengan TILs CD8+pada KPKBSK.MetodeStudi cross sectional dilakukan pada kasus KPKBSK yang teregistrasi sejak 1 Januari 2017 sampai 31 Januari 2020 di DepartemenPatologi Anatomik Fakultas Kedokteran Universitas Sriwijaya/RSU Dr. Moh. Hoesin Palembang dengan jumlah 24 sampel. Seluruhsampel dipulas menggunakan antibodi PD-L1 dan CD8, kemudian dilakukan penilaian ekspresi PD-L1 pada massa tumor dankepadatan TILs CD8+ pada area intratumor dan peritumor. Penilaian terhadap korelasi ekspresi PD-L1 dengan TILs CD8 padaKPKBSK dianalisis secara statistikHasilHanya dua subtipe yaitu adenokarsinoma dan KSS yang ditemukan pada penelitian ini. Adenokarsinoma merupakan subtipeterbanyak (54,1%), sampel kelompok didominasi oleh usia <60 tahun dan merupakan subtipe yang paling banyak mengekspresikanPD-L1 secara kuat (TPS ≥50%). Tidak terdapat korelasi signifikan antara ekspresi PD-L1 dengan TILs CD8+ pada KPKBSK(p=0,679 dan p=0,826) namun berdasarkan karakter klinikopatologik ditemukan hubungan signifikan antara PD-L1 dengan stadiumpenyakit dan antara kepadatan TILs CD8+ intratumor dengan stadium penyakit (p=0,048 dan p=0,017).KesimpulanEkspresi PD-L1 positif lebih banyak ditemukan pada subtipe adenokarsinoma dibandingkan dengan KSS. Tidak terdapat korelasiekspresi PD-L1 dengan kepadatan TILs CD8+ pada KPKBSK. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/481 10.55816/mpi.v30i3.481 Majalah Patologi Indonesia; Vol. 30 No. 3, September 2021; 314 - 322 2527-9106 0215-7284 10.55816/mpi.v30i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/481/340

oai:ojs.pkp.sfu.ca:article/482 2025-12-19T17:09:15Z patologi:ART driver

Relationship between Histomorphological Profile and Endoscopic Pictures of Reflux Esophagitis in Adults Hubungan antara Profil Histomorfologik dengan Gambaran Endoskopi Esofagitis Refluks pada Dewasa Oktamia Bernanthos, Intan Nevita Handjari, Diah Rini Reflux esophagitis; gastroesophageal reflux disease; Esohisto criteria. Esofagitis refluks; gastroesophageal refluks disease; kriteria Esohisto. BackgroundEarly diagnosis of GERD is very important because chronic reflux esophagitis is a major risk factor for Barrett esophagus, which is aprecursor lesion for esophageal adenocarcinoma. The aim of this study is to determine the frequency, demographic characteristics,histomorphological profile based on Esohisto criteria and endoscopic features of reflux esophagitis in FKUI/RSCM in 2016-2018.MethodsA cross-sectional study was conducted in Anatomical Pathology Department, Cipto Mangunkusumo General Hospital from January2016 to December 2018. Assessment criteria include demographic characteristics, histomorphological profiles, and endoscopicfeatures. Histomorphological assessment of reflux esophagitis in this study used the Esohisto criteria, by assessing basal cellhyperplasia, elevated papillae, dilated intercellular spaces and intraepithelial eosinophils. Assessment of the lesion severity isclassified as normal mucosa, mild lesion and severe lesion.ResultsThere were 65 cases of reflux esophagitis in adults over a period of 3 years in the Department of Anatomical PathologyFKUI/RSCM. Reviewing demographic data in January 2016-December 2018. The assessment criteria included demographiccharacteristics, histomorphological profiles, and endoscopic features. Histomorphological assessment of reflux esophagitis in thisstudy used the Esohisto criteria, by assessing basal cell hyperplasia, elevated papillae, dilated intercellular spaces in 65 casesfound 33 cases (50.8%) in men and 32 cases (49.2%) in women, with an average sample age of 56 years. The location of the mostlesions was distal esophagus in 30 cases (46.2%). Clinical manifestations in 13 cases (20%) were esophagitis. The most commonclinical symptoms were dysphagia in 21 cases (32.3%). Most endoscopic features were laryngopharyngeal reflux in 12 cases(18.5%), followed by grade C esophagitis in 9 cases (13.8%). The lesion severity of reflux esophagitis lesions based on Esohistocriteria found 37 cases (56.9%) mild lesion followed by severe lesion as many as 28 cases (43.1%)ConclusionThe Esohisto criteria can be used to help diagnose the lesion severity of reflux esophagitis with endoscopic pictures Latar belakangDiagnosis dini GERD sangat penting karena esofagitis refluks kronis merupakan faktor risiko utama terjadinya Barrett esofagus,yang merupakan lesi prekursor terjadinya adenokarsinoma esofagus. Tujuan penelitian ini adalah mengetahui frekuensi,karakteristik demografik, profil histomorfologi berdasarkan kriteria Esohisto dan gambaran endoskopi kasus esofagitis refluks diFKUI/RSCM tahun 2016-2018.MetodePenelitian ini merupakan penelitian deskriptif dengan desain penelitian potong lintang. Kasus esofagitis refluks diambil dari arsipDepartemen Patologi Anatomik RSCM dalam kurun waktu Januari 2016-Desember 2018. Kriteria penilaian meliputi karakteristikdemografik, profil histomorfologi, dan gambaran endoskopi. Penilaian histomorfologi esofagitis refluks dalam penelitian inimenggunakan kriteria Esohisto, dengan menilai hiperplasia sel basal (HSB), peninggian papilla (PP), dilated intercellular spaces(DIS) dan intraepitelial eosinophil (IE). Penilaian derajat lesi digolongkan menjadi mukosa normal, derajat ringan dan derajat berat.HasilDitemukan 65 kasus esofagitis refluks pada dewasa selama kurun waktu 3 tahun di Departemen Patologi Anatomik FKUI/RSCM.Penelaahan data demografik pada 65 kasus didapatkan 33 kasus (50,8 %) pada laki-laki dan 32 kasus (49,2%) pada perempuan,dengan rerata usia sampel 56 tahun. Lokasi lesi terbanyak adalah distal esofagus sebanyak 30 kasus (46,2%). Manifestasi klinispada 13 kasus (20%) berupa esofagitis. Gejala klinis tersering berupa disfagia pada 21 kasus (32,3%). Gambaran endoskopikterbanyak berupa laringofaringeal refluks sebanyak 12 kasus (18,5%), diikuti dengan esofagitis grade C sebanyak 9 kasus (13,8%).Derajat lesi esofagitis refluks berdasarkan kriteria Esohisto didapatkan 37 kasus (56,9%) derajat ringan dikuti derajat beratsebanyak 28 kasus (43,1%)KesimpulanKriteria Esohisto dapat digunakan dalam membantu menegakkan diagnosis derajat lesi esofagitis refluks dengan endoskopi. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/482 10.55816/mpi.v30i3.482 Majalah Patologi Indonesia; Vol. 30 No. 3, September 2021; 323 - 329 2527-9106 0215-7284 10.55816/mpi.v30i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/482/341

oai:ojs.pkp.sfu.ca:article/483 2025-12-19T17:09:15Z patologi:ART driver

New York Esophageal Squamous Cell Carcinoma-1 (NY-ESO-1) Expression in Synovial Sarcoma and Other Non Synovial Sarcoma as Potential Target for Immunotherapy Ekspresi New York Esophageal Squamous Cell Carcinoma-1 (NYESO-1) pada Sarkoma Sinovial dan Non Sarkoma Sinovial Lainnya Sebagai Dasar Pemilihan Pemberian Imunoterapi Ros Nirmawati, Siregar, Nurjati Chairani Evelina Kodrat synovial sarcoma, MPNST, DFSP, NY-ESO-1. sarkoma sinovial, MPNST, DFSP, NY-ESO-1 BackgroundSynovial sarcoma is a rare high grade soft tissue sarcoma. Nowdays, the available therapeutic modalities has not given asatisfactory result yet. Currently, there is a promising therapeutic strategy through immunotherapy targeting NY-ESO-1 which isexpressed on tumor. The aim of this study was comparing NY-ESO-1 immunoexpression between synovial sarcoma and itshistologic mimics i.e. malignant peripheral nerve sheath tumor (MPNST) and dermatofibrosarcoma protuberans (DFSP)MethodsA cross sectional study was done in 28 cases of synovial sarcoma, 10 cases of MPNST and 17 cases of DFSP from archievalmaterial in Department Anatomical Pathology, FMUI/RSCM from January 2013 to June 2019. Immunohistohemical stainning wasperformed using an antibody NY-ESO-1 and it was described positive if it was expressed in more than 50% of tumor with moderateto strong positive intensity.ResultsThere is a significant difference (p<0.001) in NY-ESO-1 immunoexpression among synovial sarcoma (18/28), MPNST (2/10) andDFSP (1/17). Furthermore, synovial sarcoma showed a significantly higher immunoexpression compared to MPNST (OR 7.2;p=0.016; power 68.7%) and DFSP (OR 28.8; p<0.001; power 98.9%).ConclusionSynovial sarcoma showed a higher expression of NY-ESO-1 thus makes it as a good candidates for immunotherapy. There aredifferences in the expression of NY-ESO-1 in synovial sarcoma against MPNST and DFSP. Latar belakangSarkoma sinovial adalah sarkoma jaringan lunak derajat tinggi. Modalitas terapi yang ada saat ini belum cukup memuaskansehingga mendorong perlunya modalitas terapi baru, yaitu imunoterapi yang menargetkan NY-ESO-1 yang diekspresikan oleh seltumor. Dalam penelitian, perbedaan ekspresi imunohistokimia NY-ESO-1 pada sarkoma sinovial dan diagnosis bandingnya yaitumalignant peripheral nerve sheath tumor (MPNST) dan dermatofibrosarcoma protuberans (DFSP) akan diteliti.MetodePenelitian analitik potong lintang dilakukan terhadap 28 kasus sarkoma sinovial, 10 kasus MPNST dan 17 kasus DFSP yangberasal dari Departemen Patologi Anatomik FKUI/RSCM selama Januari 2013 sampai Juni 2019. Dilakukan pulasan NY-ESO-1pada ketiga kelompok dan dikategorikan sebagai positif apabila terpulas pada lebih dari 50% sel tumor dengan intensitas positifsedang sampai kuat.HasilDitemukan perbedaan bermakna ekspresi NY-ESO-1 pada kelompok sarkoma sinovial (18/28), MPNST (2/10) dan DFSP (1/17)(p<0,001). Pada analisis lebih lanjut sarkoma sinovial memiliki ekspresi NY-ESO-1 lebih tinggi secara signifikan terhadap MPNST(OR 7,2; p=0,016; power 68,7%) dan terhadap DFSP (OR 28.8; p<0,001; power 98,9%).KesimpulanSarkoma sinovial yang mengekspresikan NY-ESO-1 berpotensi untuk mendapat pemberian imunoterapi. Terdapat perbedaanekspresi imunohistokimia NY-ESO-1 pada sarkoma sinovial terhadap MPNST dan DFSP. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2021-09-27 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/483 10.55816/mpi.v30i3.483 Majalah Patologi Indonesia; Vol. 30 No. 3, September 2021; 330 - 338 2527-9106 0215-7284 10.55816/mpi.v30i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/483/342

oai:ojs.pkp.sfu.ca:article/486 2025-12-19T17:08:29Z patologi:ART driver

Association Between Histopathological Grade of Sarcoma and Radiotherapeutical Response. Hubungan Antara Derajat Histopatologik Sarkoma dengan Respon Radioterapi Soesilowati, Eny Afiati Yulianti, Herry Aminah, Hermin Hernowo, PhD, SpPA(K), Bethy Surjawathy sarcoma, histopathological grade, radiotherapeutical response sarkoma, derajat histopatologik, respon radioterap BackgroundSarcomas are rare, aggressive tumors originating from mesenchymal tissue. Radiotherapy is currently accepted as thestandard adjuvant to the treatment of high-grade sarcomas, whereas low-grade sarcomas are controversial, but the response ofradiotherapy to sarcomas is up to 50%. The aim of this study was to determine the association between the histopathologicalgrade of sarcoma and the radiotherapeutical response.MethodsThe research design was a cross-sectional study, with retrospective analysis. The sample consisted of 80 paraffin block tissuesfrom biopsy and surgery specimens of sarcoma from January 2013 to December 2019 at Hasan Sadikin Hospital in Bandung,consisting of 45 radiosensitive samples and 35 radioresistant samples. All samples were assessed for histopathological gradeaccording to the FNCLCC. Statistical analysis used the Chi-Square test with a significant value of p <0.05. Data were processedusing SPSS version 24.0 for windows.ResultsIn the radiosensitive group, 15 (33.3%) samples were low grade sarcomas and 30 (66.7%) samples were high grade sarcoma.There was no statistically significant association between histopathological grade and radiotherapeutical response in sarcomasp> 0.05 (p = 0.304).ConclusionIn this study, the radiotherapeutical response was not associated to the histopathological grade of sarcoma. This is because ofthe sensitivity of radiotherapy is influenced by other factors including molecular characteristics. Latar belakangSarkoma merupakan tumor ganas yang berasal dari jaringan mesenkim dan bersifat agresif yang jarang ditemukan. Radioterapisaat ini diterima sebagai terapi adjuvan standar sarkoma derajat tinggi, sedangkan pada sarkoma derajat rendah masihkontroversial, namun respon radioterapi terhadap sarkoma sebesar 50%. Tujuan penelitian ini adalah untuk mengetahui hubunganderajat histopatologik sarkoma dengan respon radioterapi.MetodeDesain penelitian berupa studi crossectional, dengan analisa retrospektif. Sampel terdiri atas 80 jaringan blok parafin dari biopsi danoperasi sarkoma periode Januari 2013 hingga Desember 2019 di RS Hasan Sadikin Bandung, terdiri atas 45 sampel radiosensitifdan 35 sampel radioresisten. Semua sampel dinilai derajat histopatologik berdasarkan FNCLCC. Analisa statistik menggunakan ujiChi-Square dengan nilai signifikan p <0,05. Data diolah menggunakan program SPSS versi 24.0 for windowsHasilPada kelompok radiosensitif terdapat 15 (33,3%) sampel merupakan sarkoma derajat rendah dan 30 (66,7%) sampel merupakanderajat tinggi. Tidak terdapat hubungan yang signifikan secara statistik antara derajat histopatologik dengan respon radioterapi padasarkoma p>0,05 (p=0,304).KesimpulanPenelitian ini menunjukkan respon radioterapi pada sarkoma tidak berhubungan dengan derajat histopatologik. Hal ini disebabkankarena sensitivitas radioterapi dipengaruhi oleh faktor lain diantaranya karakter molekuler. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-01-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/486 10.55816/mpi.v31i1.486 Majalah Patologi Indonesia; Vol. 31 No. 1, Januari 2022; 339 - 343 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/486/343

oai:ojs.pkp.sfu.ca:article/487 2025-12-19T17:08:29Z patologi:ART driver

The Differences of Histomorphologic Profiles of Liver Tissue of Resipient and Donor Postpaediatric Liver Transplantation between Rejection and Non Rejection Patients in Anatomical Pathology Department of FKUI/RSCM 2010-2019 Perbedaan Profil Histomorfologik Jaringan Hati Resipien dan Donor Pascatransplantasi Hati Anak antara Kelompok Pasien Rejeksi dan Tidak Rejeksi di Departemen Patologi Anatomik FKUI/RSCM Periode 2010-2019 Perkasa, Alif Gilang Stephanie, Marini Rahadiani, Nur Handjari, Diah Rini Krisnuhoni, Ening Oswari, Hanifah liver transplantation, liver rejection, histomorphologic profiles : transplantasi hati, komplikasi rejeksi, profil histomorfologik. BackgroundLiver rejection is an immune system response of recipient in which attacking the antigen originating from a donor that can causedamage to the transplanted organ. Although the prevalence of liver rejection has decreased due to the use of immunosuppressivedrugs, it is estimated that 20-40% of recipients still experience rejection and are at risk of re-transplantation and even death. Thisstudy aims to investigating histomorphological characteristics that can play a role as risk factors for rejection by assessing thedifferences in histomorphological characteristics before transplantation between recipient groups with rejection and non-rejection inpediatric liver transplant recipients in the Department of Anatomical Pathology, Faculty of Medicine, University of Indonesia, Dr.Cipto Mangunkusumo (PA-FKUI/RSCM)MethodsThis study was an analytical study with a cross sectional design, using secondary data from the archives of the Department ofAnatomical Pathology, Faculty of Medicine, University of Indonesia, Dr. Cipto Mangunkusumo 2010-2019. The clinical andhistopathologic data obtained were analyzed using comparative statistical tests.ResultsRejection were found in 25% of recipients. Rejection were more common in the group of recipients aged >1 (75%), male (58%),cirrhosis 4C (92%) and mild portal inflammation (56%). Rejection were more common in the group of donor with male (66%) andsteatosis ≤10% (92%). There were no significant differences in the histomorphological profiles of recipients and donors with orwithout rejection.ConclusionThe histomorphologic profiles of both recipients and donors were known to be descriptively associated with complications of posttransplant liver rejection. However, in terms of analysis, there was not any significant differences PendahuluanRejeksi hati adalah respons imun tubuh terhadap antigen berasal dari donor yang dapat menyebabkan kerusakan organtransplantasi. Walaupun prevalensi rejeksi hati telah menurun akibat penggunaan obat penekan imun, namun diperkirakan 20-40%resipien masih mengalami rejeksi dan berisiko terhadap transplantasi ulang bahkan kematian. Penelitian ini bertujuan untukmempelajari karakter histomorfologik yang dapat berperan menjadi faktor risiko rejeksi, melalui penilaian perbedaan karakterhistomorfologik sebelum transplantasi antara kelompok resipien dengan komplikasi rejeksi dan tidak rejeksi pada resipientransplantasi hati anak di Departemen Patologi Anatomik Fakultas Kedokteran Universitas Indonesia, RSUPN Dr. CiptoMangunkusumo (PA-FKUI/RSCM ).MetodePenelitian ini merupakan penelitian analitik dengan desain potong lintang. Data yang digunakan adalah data sekunder berasal dariarsip Departemen Patologi Anatomik FKUI/RSCM periode 2010-2019. Data klinik dan histopatologik yang didapat dianalisis denganuji statistik komparatif.HasilKomplikasi rejeksi ditemukan pada 25% resipien. Komplikasi rejeksi lebih banyak ditemukan pada kelompok resipien berusia >1 tahun(75%), jenis kelamin laki-laki (58%), sirosis 4C (92%) dan inflamasi portal ringan (56%). Komplikasi rejeksi lebih banyak ditemukanpada kelompok donor dengan jenis kelamin lelaki (66%), steatosis ≤10% (92%). Tidak ditemukan perbedaan yang bermakna padaprofil histomorfologik resipien dan pendonor dengan rejeksi maupun tidak rejeksi.KesimpulanProfil histomorfologik pada resipien dan donor diketahui secara deskriptif berhubungan dengan komplikasi rejeksi pascatransplantasi hati. Namun secara analisis tidak ditemukan perbedaan yang bermakna. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-01-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/487 10.55816/mpi.v31i1.487 Majalah Patologi Indonesia; Vol. 31 No. 1, Januari 2022; 344 - 350 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/487/344

oai:ojs.pkp.sfu.ca:article/488 2025-12-19T17:08:29Z patologi:ART driver

Clinicopathological Characteristics and the Relationship between Histological Grade with Luminal B Breast Cancer in RSUP Mohammad Hoesin within 2014-2016 Karakteristik Klinikopatologik serta Hubungan Derajat Keganasan dengan Subtipe Molekular Luminal B pada Pasien Karsinoma Payudara di RSUP Mohammad Hoesin Palembang Tahun 2014-2016 Awaliah, Reihan Putri Dewi, Citra Puspita Rasyid, Riana Sari breast carcinoma, molecular subtype, luminal B, histopathological grade Karsinoma payudara, subtipe molekular, luminal B, derajat keganasan BackgroundBreast carcinoma is the second highest malignant disease that causes death in Indonesia with 58,256 new cases in 2018. LuminalB has more aggressive clinical behavior and is considered as having unfavorable prognosis. This study aimed to determine theclinicopathological characteristics and the relationship histological grade with breast carcinoma subtype molecular luminal B.MethodsThis is a descriptive analytic study with cross sectional design using secondary data of breast carcinoma patients registered atRSUP Mohammad Hoesin Palembang during the period of January 1st, 2014 to December 31st, 2016.ResultsFrom 903 breast carcinoma patients in Mohammad Hoesin Hospital Palembang during 2014 to 2016, 43.63% had luminal Bmolecular subtype. The highest incidence and prevalence washappened in year 2016, whichwere 4.7 and 4.4 per 10 breastcarcinoma patients, respectively. The majority of breast carcinoma of luminal B molecular subtype occurs in the group of patientsaged ≥40 years (81.7%), female (98.7%), Malay race (100%), marital status (98.8%), housewives (68.3%), histopathologicalcarcinoma of invasive non-specific type (75.9%), high grade/ grade III (63.7%), localized (60.6%), no metastases (60.0%), rightsided tumor laterality (50.8%) and with negative HER2 (65.2%). There was a significant relationship between the histopathologicalgrade and breast carcinoma luminal B molecular subtype (p=0.009).ConclusionThe incident of breast carcinoma molecular subtype luminal B at Mohammad Hoesin Hospital Palembang has increased in the lastthree years with the poor clinicopathological characteristics. Latar belakangKarsinoma payudara merupakan penyakit keganasan tertinggi kedua yang menyebabkan kematian di Indonesia pada tahun 2018dengan jumlah kasus baru sebanyak 58.256. Subtipe molekuler luminal B karsinoma payudara memiliki perilaku klinis yang lebihagresif dan dianggap memiliki prognosis yang tidak menguntungkan dibandingkan dengan subtipe luminal A. Penelitian inibertujuan untuk mengetahui karakteristik klinikopatologik serta hubungan derajat keganasan dengan subtipe molekular luminal Bkarsinoma payudara.MetodePenelitian ini bersifat deskriptif analitik dengan metode cross sectional menggunakan data sekunder pasien karsinoma payudarayang terdaftar di RSUP Mohammad Hoesin Palembang periode 1 Januari 2014-31 Desember 2016.HasilDari 903 pasien karsinoma payudara di RSUP Mohammad Hoesin Palembang pada tahun 2014-2016, sebanyak 43,63% diantaranya merupakan karsinoma payudara subtipe molekular luminal B. Insidensi dan prevalensi tertinggi berada pada tahun 2016yaitu 4,7 dan 4,4 per 10 pasien karsinoma payudara. Mayoritas karsinoma payudara subtipe molekular luminal B terjadi padakelompok pasien dengan usia ≥40 tahun (81,7%), berjenis kelamin perempuan (98,7%), ras Melayu (100%), status menikah(98,8%), pekerjaan ibu rumah tangga (68,3%), tipe histopatologi karsinoma invasif non spesifik (75,9%), derajat histopatologitinggi/grade III (63,7%), dengan perluasan tumor yang terlokalisir (60,6%), tidak terdapat metastasis (60,0%), lateralitas tumor sisikanan (50,8%) dan dengan status HER2 negatif (65,2%). Derajat keganasan memiliki hubungan yang signifikan dengan subtipemolekular luminal B karsinoma payudara (p=0,009).KesimpulanKarsinoma payudara subtipe molekular luminal B di RSUP Mohammad Hoesin Palembang mengalami peningkatan pada tiga tahunterakhir dengan karakteristik klinikopatologik yang buruk. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-01-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/488 10.55816/mpi.v31i1.488 Majalah Patologi Indonesia; Vol. 31 No. 1, Januari 2022; 351 - 358 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/488/345

oai:ojs.pkp.sfu.ca:article/489 2025-12-19T17:08:29Z patologi:ART driver

Clinicopathological Profile of Invasive Breast Cancer with Distant Metastases in Anatomical Pathology Department FKUI/RSCM 2019 Profil Klinikopatologik Karsinoma Payudara Invasif Metastasis Jauh di Departemen Patologi Anatomik FKUI/RSCM Tahun 2019 Kurnianingrum, Laurencia Leny Tjahjadi, Hartono Invasive breast carcinoma, distant metastases, clinicopathologic Karsinoma invasif payudara, metastasis jauh, klinikopatologik. BackgroundInvasive breast carcinoma (IBC) is the highest incidence and is the leading cause of malignancy-related death in women in theworld. Bones are the most common sites of IBC metastases. This study aims to provide clinical and histological characteristic datain cases of distant metastastic IBC in the Anatomical Pathology Department, Faculty of Medicine, Universitas Indonesia, Dr. CiptoMangunkusumo General Hospital (PA-FKUI/RSCM).MethodsThis is descriptive research with cross-sectional design, using secondary data from the archives of PA-FKUI/RSCM, starting fromJanuary 1, 2019 to December 31, 2019.ResultsDistant metastases of IBC was found 65.2% as bone metastases, 46.1% as pulmonary metastases, 26.1% as liver metastases, and8.7% as brain metastases. The largest age group was 40-59 years, median age was 49 years with range 27-78 years. The mostcommon of the classification of primary tumor size was 54.8% T4, and 87% unilateral cases. Most histologic subtypes were 90.4%cases no special type, then lobular and mucinous subtypes. Lymphovascular invasion was 24.3% cases. The most commonmolecular subtype was luminal B Luminal and mostly as bone metastases and triple negative breast cancer (TNBC) in lungmetastases. The greatest histological grade was grade 2.ConclusionBone was the most common IBC metastatic. The most common of the classification of primary tumor size was T4 and histologicsubtype was no special. Luminal B was the most common molecular subtypes and the highest was bone metastases. Latar belakangKarsinoma payudara invasif (KPI) merupakan keganasan payudara yang paling sering didiagnosis dan merupakan penyebabkematian tertinggi pada wanita di dunia. Metastasis KPI yang paling sering adalah metastasis tulang. Penelitian ini bertujuan untukmemberikan data karakteristik klinis dan histopatologik pada kasus-kasus KPI metastasis jauh di Departemen Patologi AnatomikFakultas Kedokteran Universitas Indonesia, RSUPN Dr. Cipto Mangunkusumo (PA-FKUI/RSCM).MetodePenelitian deskriptif dengan desain penelitian potong lintang dengan data sekunder dari arsip Departemen PA-FKUI/RSCM mulaidari 1 Januari 2019 sampai dengan 31 Desember 2019.HasilKasus KPI metastasis jauh ditemukan sebanyak 65,2% metastasis tulang, 46,1% metastasis paru, 26,1% metastasis hati, dan 8,7%metastasis otak. Median usia adalah 49 tahun dengan rentang usia 27-78 tahun. Klasifikasi ukuran tumor primer terbanyak adalah54,8% kasus T4, dan 87% kasus unilateral. Subtipe histologik paling banyak adalah 90,4% kasus no special type, diikuti subtipelobular dan mucinous. Invasi limfovaskular ditemukan pada 24,3% kasus. Subtipe molekular yang paling banyak adalah subtipeluminal B dan paling banyak pada metastasis tulang, sedangkan triple negative breast cancer (TNBC) pada metastasis paru.Derajat histologik terbanyak adalah derajat 2.KesimpulanLokasi metastasis jauh tersering KPI adalah tulang dengan klasifikasi ukuran tumor primer terbanyak adalah T4 dan subtipehistologik terbanyak adalah no special type. Subtipe molekular terbanyak adalah subtipe luminal B dan tertinggi adalah metastasistulang. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-01-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/489 10.55816/mpi.v31i1.489 Majalah Patologi Indonesia; Vol. 31 No. 1, Januari 2022; 359 - 367 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/489/346

oai:ojs.pkp.sfu.ca:article/490 2025-12-19T17:08:29Z patologi:ART driver

The Association of VEGF Expression with Degree of Differentiation and Lymphovascular Invasion in Colorectal Adenocarcinoma Hubungan Ekspresi Vascular Endothelial Growth Factor (VEGF) dengan Derajat Diferensiasi dan Invasi Limfovaskular pada Adenokarsinoma Kolorektal Liana, Nana Hilbertina, Noza Devianti, Loli Yetti, Husna colorectal adenocarcinoma, VEGF expression, degree of differentiation, lymphovascular invasion. adenokarsinoma kolorektal, ekspresi VEGF, derajat diferensiasi, invasi limfovaskular. BackgroundColorectal carcinoma is the third most common malignancy in the world. Colorectal carcinoma is a heterogeneous tumor withdifferent clinical pathologic features and prognostic values. For the same tumor-stage, patients could have difference prognosis andit has been suggested that the angiogenesis might be correlated with the prognosis, especially expression of vascular endothelialgrowth factor (VEGF) as the main pro-angiogenic factor. High VEGF expression in colorectal adenocarcinoma is associated withincreased blood vessels in invasive tumor area, cell proliferation and metastases. However, relation VEGF expression with thedegree of differentiation and lymphovascular invasion is not known.MethodsThis was a retrospective observational study with cross sectional approach. Samples were obtained from 39 paraffin blocks withdiagnosis adenocarcinoma not otherwise specific (NOS) in four Anatomical Pathology Laboratory in West Sumatera 2018 andevaluated for degree of differentiation and lymphovascular invasion. VEGF expressions in tumor cell were analyzed usingimmunohistochemistry staining. Bivariate statistical analysis used Fisher's Exact test and value p<0.05 was considered significant.ResultsColorectal adenocarcinoma with high grade differentiation entirely had high VEGF expression (100%), while low gradedifferantiation with high VEGF expression was 60.7%. Lymphovascular invasion positive was mostly found with high VEGFexpression (80.6%). Statistical analysis showed significant association between VEGF expression with degree of differentiation(p=0.017) and lymphovascular invasion (p=0.028).ConclusionThe conclusion was VEGF expression had significant association with degree of differentiation and lymphovascular invasion ofcolorectal adenocarcinoma. Latar belakangKarsinoma kolorektal merupakan keganasan ketiga terbanyak di dunia. Karsinoma kolorektal adalah tumor heterogen dengangambaran klinikopatologik dan nilai prognostik berbeda. Pada stadium yang sama, penderita memiliki perjalanan penyakit berbedasehingga diduga terdapat faktor prognostik lain yang mempengaruhi prognosis selain stadium tumor, nodus dan metastasis (TNM).Salah satu faktor tersebut adalah angiogenesis, terutama vascular endothelial growth factor (VEGF) sebagai faktor proangiogenikutama. Ekspresi VEGF tinggi pada adenokarsinoma kolorektal dikaitkan dengan peningkatan jumlah pembuluh darah di areainvasif, proliferasi sel dan metastasis jauh. Namun belum diketahui bagaimana hubungan VEGF dengan derajat diferensiasi daninvasi limfovaskular.MetodePenelitian ini merupakan penelitian observasional dengan pendekatan cross sectional. Sampel penelitian ini adalah 39 blok parafindengan diagnosis adenocarcinoma not otherwise specific (NOS) di empat Laboratorium Patologi Anatomik di Sumatera Barat tahun2018. Kemudian dilakukan penilaian derajat diferensiasi dan invasi limfovaskular. Ekspresi VEGF pada sel tumor dinilai denganpewarnaan imunohistokimia. Analisis statistik bivariat menggunakan uji Fisher's Exact dengan p<0,05 dianggap bermakna.HasilPada adenokarsinoma kolorektal diferensiasi high grade, seluruhnya dengan ekspresi VEGF tinggi (100%) sedangkan padadiferensiasi low grade ekspresi VEGF tinggi sebesar 60,7%. Pada adenokarsinoma kolorektal dengan invasi limfovaskular positiflebih banyak ditemukan ekspresi VEGF tinggi (80,6%). Analisis statistik menunjukkan hubungan bermakna antara ekspresi VEGFdengan derajat diferensiasi (p=0,017) dan dengan invasi limfovaskular (p=0,028).KesimpulanEkspresi VEGF pada sel tumor mempunyai hubungan bermakna dengan derajat diferensiasi dan invasi limfovaskular padaadenokarsinoma kolorektal. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-01-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/490 10.55816/mpi.v31i1.490 Majalah Patologi Indonesia; Vol. 31 No. 1, Januari 2022; 368 - 375 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/490/347

oai:ojs.pkp.sfu.ca:article/491 2025-12-19T17:08:29Z patologi:ART driver

Diagnosis Accuracy of Fine Needles Aspiration Assessment in Bone Tumors in the Department of Anatomical Pathology FKUI/RSCM 2015-2019 Akurasi Diagnosis Pemeriksaan Biopsi Aspirasi Jarum Halus Tumor Tulang di Departemen Patologi Anatomik FKUI/RSCM Tahun 2015- 2019 Putri, Vabiayu Stephanie, Marini bone tumor, cytology, fine needle aspiration biopsy, accuracy Kata kunci: tumor tulang, sitologi, biopsi aspirasi jarum halus, akurasi BackgroundFine needle aspiration biopsy (FNAB) is a safe and rapid technique and has an important role in making the preoperative diagnosisof bone tumors because it can help clinicians determine treatment for patients. This retrospective study aims to assess the accuracyof the FNAB cytological examination compared with the histopathological examination in preoperative diagnosis of bone tumors.MethodsThe data comes from the archives of the Department of Anatomic Pathology, FKUI/RSCM 2015-2019 in the form of a cytologicalexamination of FNAB lesions on bone. Then the histopathological examination data were searched for each cytology preparation.There were 133 pairs of cytology, and histopathology preparations. The preparations were reviewed by the investigator andsupervisor, assessed for the suitability of the cytologic diagnosis, and compared with the histopathological diagnosis.ResultsThere were 84 (63.2%) cases of malignant tumors, 44 (33.1%) cases of benign tumors, and 5 (3.7%) cases of non-tumors. Thediagnostic test results showed an accuracy rate of 86.5% with a sensitivity of 85.9%, a specificity of 100%, a positive predictivevalue (PPV) of 100%, and a negative predictive value (NPV) of 21.7%.ConclusionFNAB cytology examination of bone tumors that have adequate sample quality, supported by complete clinical information andinformative radiological images can produce an accurate preoperative diagnosis Latar belakangBiopsi aspirasi jarum halus (BAJH) merupakan teknik yang aman dan cepat serta memiliki peran yang penting dalam menegakkandiagnosis preoperatif tumor tulang karena dapat membantu klinisi menentukan tata laksana bagi pasien. Tujuan telaah retrospektifini untuk menilai akurasi pemeriksaan sitologi dengan teknik BAJH dibandingkan dengan pemeriksaan histopatologi dalammendiagnosis tumor tulang preoperatif.MetodeData berasal dari arsip Departemen Patologi Anatomik FKUI/RSCM tahun 2015-2019 berupa pemeriksaan sitologi BAJH lesi padatulang. Kemudian dilakukan pencarian data pemeriksaan histopatologi dari setiap sediaan sitologi. Didapatkan 133 sedian sitologidan histopatologi berpasangan. Sediaan dibaca ulang oleh peneliti dan pembimbing, dinilai kesesuaian diagnosis sitologinya dandibandingkan dengan diagnosis histopatologinya.HasilDitemukan 84 (63,2%) kasus tumor ganas, 44 (33,1%) kasus tumor jinak dan 5 (3,7%) kasus non-tumor. Hasil uji diagnostikmenunjukkan tingkat akurasi 86,5% dengan sensitivitas 85,9%, spesifisitas 100%, nilai prediksi positif (NPP) 100% dan nilai prediksinegatif (NPN) 21,7%.KesimpulanAkurasi diagnosis pemeriksaan BAJH pada tumor tulang sebesar 86,5%. Pemeriksaan sitologi BAJH pada tumor tulang yangmemiliki kualitas sampel yang adekuat, ditunjang dengan keterangan klinis yang lengkap dan gambaran radiologi yang informatifdapat menghasilkan diagnosis preoperatif yang akurat. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-01-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/491 10.55816/mpi.v31i1.491 Majalah Patologi Indonesia; Vol. 31 No. 1, Januari 2022; 376 - 384 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/491/348

oai:ojs.pkp.sfu.ca:article/492 2025-12-19T17:08:29Z patologi:ART driver

Granulomatous Mastitis: A Diagnostic Challenge Mastitis Granulomatosa: Sebuah Tantangan Diagnosis Dewi, Citra Amalia, Ella Kurniawati, Nyiayu Fauziah Maria Ulfa,, Maria Ulfa, Pratama, Eka Putra Murti, Krisna granulomatous mastitis, PCR, specific primer IS6110, PCR product of 200 bp mastitis granulomatosa, PCR, primer spesifik IS6110, produk PCR 200 bp BackgroundGranulomatous mastitis (GM) is often found in reproductive-age women. These patients are treated by the administration ofantituberculosis drugs. It is difficult to determine the exact cause of GM cytologically and histopathologically. Various etiologies i.e.,tuberculosis, sarcoidosis, foreign bodies and immunological diseases may provide granulomatous inflammation features in thetissue. High-sensitivity tests such as PCR can help to detect the presence of Mycobacterium tuberculosis (MT) as one of possibleetiologic agent. Accurate diagnosis will improve the treatment quality thus a better quality of life. The aim of this study was todetermine whether the granulomatous mastitis patients who referred to the Department of Anatomic Pathology Faculty of MedicineUniversitas Sriwijaya/Dr. Moh. Hoesin Palembang Hospital were caused by MT.MethodsA descriptive observational study was performed. Thirty paraffin blocks of GM patients during the period of 1st October 2018 to 29thSeptember 2020 were collected. DNAs were extracted from tissue scrapings of the slides. Then, PCR analysis was conducted usinga specific primer IS6110 with around 200 bp PCR product.ResultsThe mean age of the patients was 40.8 years (range: 23-67 years). There were six samples (20%) of GM produced the expectedamplicon. However, only three of six samples morphologically consistent with tuberculosis.ConclusionGranulomatous inflammatory reactions in the breast are not only caused by tuberculosis. Further study is needed to determine theexact cause of this lesion. Despite high costs, PCR examination may become an alternative approach in determining the etiology ofGM. Latar belakangMastitis granulomatosa (MG) merupakan lesi yang sering dijumpai pada wanita usia reproduksi. Sering kali terapi pasien MGmerujuk pada pemberian obat antituberkulosa. Pada pemeriksaaan sitologi dan histopatologi sulit untuk menentukan apakahpenyebab pasti dari MG. Berbagai penyebab seperti tuberkulosis, sarcoidosis, benda asing bahkan penyakit imunologi dapatmemberikan gambaran granulomatosa pada jaringan. Pemeriksaan yang memiliki sensitivitas tinggi seperti PCR dapat membantumemastikan apakah lesi ini disebabkan oleh bakteri Mycobacterium tuberculosis (MT). Penegakkan diagnosis yang tepat akanmeningkatkan kualitas terapi yang berimbas pada meningkatnya kualitas hidup pasien. Tujuan penelitian ini untuk mengetahuiapakah lesi mastitis granulomatosa pada pasien-pasien di RSMH disebabkan oleh bakteri MT.MetodePenelitian ini menggunakan metode deskriptif observasional. Sebanyak 30 sampel penelitian berupa blok parafin pasien MG yangdatang ke Departemen Patologi Anatomik Fakultas Kedokteran Universitas Sriwijaya (FK UNSRI)/RS. Dr. Moh. Hoesin Palembangperiode 1 Oktober 2018-29 September 2020. Ekstraksi DNA berasal dari kerokan jaringan pada slaid, kemudian dilakukan analisisPCR setiap sediaan menggunakan primer spesifik IS6110 dan produk PCR sekitar 200 bp.HasilRerata usia sampel adalah 40,8 tahun (rentang: 23-67). Dari analisis PCR didapatkan sebanyak enam sampel (20%) MG yangmenghasilkan amplikon yang diharapkan yaitu sekitar 200 bp. Namun hanya tiga dari enam sampel menunjukkan gambaranhistopatologi spesifik MG akibat tuberkulosis.KesimpulanReaksi radang granulomatosa pada payudara tidak hanya disebabkan oleh infeksi tuberkulosis, walaupun gambaran histopatologijaringan menunjukkan sebaliknya. Perlu pemeriksaan lanjutan untuk menentukan apakah tuberkulosis adalah penyebab dari lesi ini.Walaupun biaya tinggi namun pemeriksaan PCR dapat dijadikan alternatif dalam menentukan etiologi dari MG. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-01-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/492 10.55816/mpi.v31i1.492 Majalah Patologi Indonesia; Vol. 31 No. 1, Januari 2022; 385 - 391 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/492/349

oai:ojs.pkp.sfu.ca:article/493 2025-12-19T17:08:29Z patologi:ART driver

Association between Molecular Subtype of Invasive Breast Carcinoma with grade, lymphovascular invasion and lymph node metastasis in the Department of Anatomic Pathology FKUI/RSCM 2019 Hubungan Subtipe Molekular pada Karsinoma Payudara Invasif dengan Grade, Invasi Limfovaskular dan Metastasis KGB di Departemen Patologi Anatomik FKUI/RSCM Tahun 2019 Bethania, Kristina Anna Rustamadji, Primariadewi : Invasive breast carcinoma, immunohistochemistry, molecular subtypes. Karsinoma invasif payudara, imunopatologik, subtipe molekular. BackgroundBreast cancer is classified into molecular subtypes: luminal A, luminal B, HER2 enriched and triple negative breast cancer based onits immunohistochemical profile. This study aims to assess the relationship between histologic grade, lymphovascular invasion andlymph node metastasis with molecular subtypes in invasive breast carcinoma at Cipto Mangunkusumo Hospital from January 1,2019 to December 31, 2019.MethodsA descriptive, cross-sectional study was conducted in Anatomical Pathology Department, FKUI/RSCM over a period of January toDecember 2019. A total of 652 histologic specimens with a diagnosis of invasive breast carcinoma which were immunostained withER, PR, HER2, and Ki67 were reviewed, then grouped into the appropriate molecular subtypes. A Chi-square test was performed toassess the association between histologic grade, lymphovascular invasion and regional lymph node metastases with molecularsubtypes.The p-value <0.05 was statistically significant.ResultsThe molecular subtypes of breast cancer, from the highest to the least number, were luminal A (40.2%), luminal B (37.7%), TNBC(13.6%), and HER2 enriched (8.6%). There was a significant association between grade and molecular subtypes of breast cancer.(p=<0.001). There was a significant association between lymph node metastasis and molecular subtypes of breast cancer(p=0.038).ConclusionThe most common molecular subtype of invasive breast cancer found was Luminal A. There was a significant association betweenlymph node grade and metastasis and the molecular subtype of breast cancer. Latar belakangKanker payudara dapat dikelompokkan menjadi subtipe molekular luminal A, luminal B, HER2 enriched dan triple negative breastcancer berdasarkan profil imunopatologiknya. Penelitian ini bertujuan untuk menilai karakteristik imunopatologik karsinomapayudara invasif di Rumah Sakit Cipto Mangunkusumo Jakarta, Indonesia sejak 1 Januari hingga 31 Desember 2019.MetodePenelitian deskriptif dengan desain penelitian potong lintang dengan data sekunder dari arsip Departemen Patologi AnatomikFKUI/RSCM sejak 1 Januari 2019 sampai dengan 31 Desember 2019. Dilakukan penilaian terhadap 652 spesimen histologi dengandiagnosis karsinoma payudara invasif yang telah dilakukan pulasan imunohistokimia ER, PR, HER2, dan Ki67, kemudiandikelompokkan menjadi subtipe molekular payudara yang sesuai. Dilakukan uji analitik Chi-Square untuk menilai hubungan grade,invasi limfovaskular dan metastasis kelenjar getah bening regional dengan subtipe molekular. Nilai p<0,05 dinyatakan bermaknasecara statistik.HasilSubtipe molekular kanker payudara dari yang terbanyak hingga yang jumlahnya paling sedikit yaitu luminal A (40,2%), luminal B(37,7%), TNBC (13,6%) dan HER2 enriched (8,6%). Terdapat hubungan bermakna antara grade dengan subtipe molekular kankerpayudara (p=<0,001). Terdapat hubungan bermakna antara metastasis kelenjar getah bening dengan subtipe molekular kankerpayudara (p=0,038).KesimpulanSubtipe molekular kanker payudara terbanyak yang ditemukan adalah Luminal A. Terdapat hubungan bermakna antara grade danmetastasis kelenjar getah bening dengan subtipe molekular kanker payudara. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-01-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/493 10.55816/mpi.v31i1.493 Majalah Patologi Indonesia; Vol. 31 No. 1, Januari 2022; 392 - 399 2527-9106 0215-7284 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/493/350

oai:ojs.pkp.sfu.ca:article/498 2025-12-19T17:08:06Z patologi:ART driver

Sinonasal Papilloma and Its Associated Malignancy: Study from a Tertiary Referral Hospital in Indonesia Papiloma Sinonasal dan Keganasan Terkait Papiloma Sinonasal: Studi dari Pusat Rujukan Tersier di Indonesia Sitinjak, David Rachmadi, Lisnawati Billianti, Yayi Dwina papiloma sinonasal, papiloma inverted, perubahan keganasan Sinonasal papilloma, inverted papilloma, malignant transformation Background Sinonasal papilloma is a benign tumor arising from Schneiderian epithelia in sinonasal mucosa. It has the propensity towards malignant transformation. This preliminary study aims to report clinicopathological aspects of sinonasal papilloma and its malignant transformation rate. Methods A descriptive, cross-sectional study was conducted in Anatomical Pathology Department, FKUI/RSCM over a period of 2014-2019) A total of 127 histologic specimens with diagnosis of sinonasal papilloma obtained from 80 cases. Results The prevalence of sinonasal papilloma among all sinonasal neoplasms is 17.6%. Inverted papilloma was the most common histologic type 91.3%, exophytic papilloma 6.3% and oncocytic papilloma 2.5%, among male (70%), age rate 50 years old. Malignant transformation of squamous cell carcinoma 12.5%, and recurrence 21.3%, nasal congestion was the most complaint 50%. Cases were usually in T3 of Krouse stage 37.5% and managed with functional sinus endoscopic surgery 51.3%. Histological parameters were grade II 54.4%, surface keratinization 17.5%, bony trabeculae 41.3%, nasal polyps 20% and dense lymphocytic infiltrates 53.8%, foam cell infiltrates 2.5%. Mild to moderate dysplasia 8.8% and severe dysplasia 18.8%. Conclusion Sinonasal papilloma was rare sinonasal benign tumor. Inverted papilloma type was local aggressive, recurrence, dysplastic and malignant transformation, among male, age 50 years old, main complaint of nasal congestion, in Krouse stage T3, and managed by Functional Sinus Endoscopy Surgery (FSES Latar belakang Papiloma sinonasal merupakan tumor jinak yang berasal dari epitel permukaan yang melapisi rongga sinonasal. Papiloma sinonasal memiliki kecenderungan untuk mengalami transformasi keganasan. Penelitian ini merupakan penelitian pendahuluan untuk mengetahui karakteristik klinikopatologik papiloma sinonasal dan angka perubahan keganasan pada papilloma sinonasal. Metode Penelitian bersifat deskriptif dengan desain potong lintang dilakukan dengan data sekunder kasus papiloma sinonasal dari arsip Departemen Patologi Anatomik FKUI/RSCM tahun 2014-2019. Dilakukan telaah ulang pada 127 spesimen histologik dengan diagnosis papiloma sinonasal yang berasal dari 80 kasus. Hasil Papiloma sinonasal prevalensi 17,6% dari seluruh neoplasma sinonasal. Jenis histologik terbanyak adalah papiloma inverted 91,3%, papiloma eksofitik 6,3% dan onkositik 2,5%; terutama pada pria sebanyak 70%, rerata usia 50 tahun. Perubahan papiloma sinonasal menjadi KSS sinonasal sebanyak 12,5%; rekurensi 21,3%; dengan keluhan utama hidung tersumbat 50,0%. Kasus berada pada stadium T3 menurut system Krouse 37,5% dan penatalaksanaan dengan metode bedah endoskopik sinus fungsional (BESF) 51,3%. Parameter histopatologik derajat II 53,4%, keratinisasi 17,5%, trabekula tulang 41,3%, polip hidung 20%, dan sebukan limfosit keras 53,8%, sebukan sel buih 2,5%. Gambaran displasia ringan-sedang 8,8%, dan displasia keras 18,8%. Kesimpulan Papiloma sinonasal merupakan tumor sinonasal jinak yang jarang. Tipe papiloma inverted bersifat agresif lokal, rekuren, displasia dan transformasi keganasan, terutama pada pria rerata usia 50 tahun dengan keluhan utama hidung tersumbat pada stadium krouse T3, dan tatalaksana utama adalah BESF Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-05-02 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/498 10.55816/mpi.v31i2.498 Majalah Patologi Indonesia; Vol. 31 No. 2, Mei 2022; 400 - 408 2527-9106 0215-7284 10.55816/mpi.v31i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/498/380 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/499 2025-12-19T17:08:06Z patologi:ART driver

The Correlation of VEGF Expression with Grade of Differentiation and Lymphovascular Invasion in Bladder Infiltrating Urothelial Carcinoma Hubungan Ekspresi Vascular Endothelial Growth Factor (VEGF) dengan Derajat Diferensiasi dan Invasi Limfovaskular pada Karsinoma Urotelial Infiltratif Kandung Kemih Utami, Dini Andri Agus, Salmiah Yenita Yetti, Husna bladder infiltrating urothelial carcinoma, VEGF expression, grade of differentiation, lymphovascular invasion karsinoma urotelial infiltratif kandung kemih, ekspresi VEGF, derajat diferensiasi, invasi limfovaskular Background Urothelial carcinoma is the most common malignancy in the bladder with an incidence up to 95% and 70-85% of them are bladder infiltrating urothelial carcinoma. To improve the management of bladder urothelial carcinoma, prognostic factors are required. One such factor is angiogenesis, which affects growth, development and metastasis. Vascular endothelial growth factor (VEGF) is the main pro-angiogenic factor to control angiogenesis. Expression of VEGF is correlated with progression of bladder infiltrating urothelial carcinoma, such as grade and lymphovascular invasion. The aim of this study was to determine the correlation of VEGF expression with grade of differentiation and lymphovascular invasion in bladder infiltrating urothelial carcinoma. Methods This research was a retrospective observational cross sectional study with 48 cases of bladder infiltrating urothelial carcinoma in four Anatomical Pathology Laboratory in West Sumatera 2018 and 2019. Samples were reevaluated of HE slide for grade of differentiation and lymphovascular invasion. VEGF expressions in tumor cell s were analyzed using immunohistochemistry staining. Bivariate statistical analysis used Fisher's Exact test and value p<0.05 was considered significant. Results Bladder infiltrating urothelial carcinoma high grade had more positive VEGF expression (91.7%), while low grade had more negative VEGF expression (58.3%). Lymphovascular invasion positive was mostly found with positive VEGF expression (75%). Statistical analysis showed significant correlation between VEGF expression with grade of differentiation (p=0.001) and lymphovascular invasion (p=0.004). Conclusion The conclusion was VEGF expression had significant correlation with grade of differentiation and lymphovascular invasion of bladder infiltrating urothelial carcinoma.   Latar belakang Karsinoma urotelial merupakan keganasan terbanyak di kandung kemih dengan insiden mencapai 95% dan 70-85% diantaranya merupakan karsinoma urotelial infiltratif kandung kemih. Untuk meningkatkan penanganan pada karsinoma urotelial kandung kemih, diperlukan adanya faktor prognostik. Salah satu faktor tersebut adalah angiogenesis, yang mempengaruhi pertumbuhan, perkembangan dan metastasis. Vascular endothelial growth factor (VEGF) adalah faktor pro-angiogenik utama untuk mengontrol angiogenesis. Ekspresi VEGF berhubungan dengan progresivitas karsinoma urotelial infiltratif kandung kemih, seperti derajat diferensiasi, dan invasi limfovaskular. Tujuan penelitian ini untuk mengetahui hubungan ekspresi VEGF dengan derajat diferensiasi dan invasi limfovaskular pada karsinoma urotelial infiltratif kandung kemih. Metode Penelitian ini merupakan penelitian observasional dengan pendekatan cross sectional. Sampel penelitian sebanyak 48 kasus karsinoma urotelial infiltratif kandung kemih di empat Laboratorium Patologi Anatomik di Sumatera Barat tahun 2018 dan 2019. Sampel diperoleh dari kasus karsinoma urotelial infiltratif kandung kemih kemudian dilakukan reevaluasi slaid Hematoxilyn Eosin (H&E) berupa derajat diferensiasi dan invasi limfovaskular. Ekspresi VEGF pada sel tumor dinilai dengan pewarnaan imunohistokimia. Analisis statistik bivariat menggunakan uji Fisher's Exact dengan p<0,05 dianggap bermakna. Hasil Karsinoma urotelial infiltratif kandung kemih derajat tinggi lebih banyak dengan ekspresi VEGF positif (91,7%), sedangkan pada derajat rendah lebih banyak dengan ekspresi VEGF negatif (58,3%). Pada invasi limfovaskular positif lebih banyak ditemukan ekspresi VEGF positif (75%). Analisis statistik menunjukkan hubungan bermakna antara ekspresi VEGF dengan derajat diferensiasi (p=0,001) dan invasi limfovaskular (p=0,004). Kesimpulan Ekspresi VEGF mempunyai hubungan bermakna dengan derajat diferensiasi dan invasi limfovaskular pada karsinoma urotelial infiltratif kandung kemih. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/499 10.55816/mpi.v31i2.499 Majalah Patologi Indonesia; Vol. 31 No. 2, Mei 2022; 409 - 416 2527-9106 0215-7284 10.55816/mpi.v31i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/499/381 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/501 2025-12-19T17:08:06Z patologi:ART driver

Clinicopathological Characteristics of Cervical Squamous Cell Carcinoma and Correlation with Tumor-Associated Neutrophil Karakteristik Klinikopatologik Karsinoma Sel Skuamosa Serviks dan Hubungannya dengan Tumor-Associated Neutrophil Alisa, Nanda Safira Joko M Dewi, Citra Susilawati Edi Pudjo, Ika Kartika cervical squamous cell carcinoma, tumor-associated neutrophil, clinicopathological characteristics, prognostic squamous cell carcinoma serviks, tumor-associated neutrophil, karakteristik klinikopatologik, prognostik. BackgroundCervical carcinoma is one of the most common cancers in women, according to data from World Health Organization (WHO).Cervical carcinoma ranks the fourth most deadly type of cancer. Squamous cell carcinoma is the most common type of this cervicalcancer and it is generally caused by Human Papilloma Virus (HPV) types 16 and 18. Tumor-Associated Neutrophil (TAN) are foundin various types of cancer, the density of TAN can determine the prognostic type of tumor or cancer in each individual. This studywas aimed to identify correlation between clinicopathological characteristics of cervical squamous cell carcinoma (SCC) and TAN incervical SCC patients registered at RSUP Dr. Mohammad Hoesin Palembang.MethodsThis type of research is analytic with a cross-sectional method. Data collection in this study used a 37 slides archive which werediagnosed as cervical squamous cell carcinoma in Anatomic Pathology in RSUP Dr. Mohammad Hoesin Palembang for the periodof 2017-2019.ResultsFrom 37 cervical SCC patients, the clinicopathological characteristics of the majority of cervical SCC patients occurred in the agegroup of 51 year-old (51.4%), with histopathological type of non-keratinizing SCC (75,7%) and the most frequent differentiationdegree was moderately differentiated (75,7%). The TAN density was mostly found at low TAN densities (73%). There wassignificant correlation between age and TAN density (p=0.035) and there was no significant correlation between age and TANdensity (p=0.269).ConclusionIt was explained that the higher the degree of differentiation, the lower the density value, however the neutrophils in this studycannot be ascertained whether the neutrophils of anti-tumor (N1) or pro-tumor (N2) characteristic PendahuluanKarsinoma serviks merupakan salah satu kanker yang sering dijumpai pada wanita. Menurut data World Health Organization(WHO) karsinoma serviks menempati urutan ke empat dari jenis kanker yang mematikan. Tipe terbanyak dari karsinoma ini adalahtipe karsinoma sel skuamosa dan umumnya disebabkan oleh Human Papilloma Virus (HPV) tipe 16 dan 18. Tumor-AssociatedNeutrophil (TAN) dijumpai pada berbagai jenis kanker, di mana kepadatan TAN dihubungkan dengan prognostik kanker padamasing-masing individu. Penelitian ini bertujuan untuk mengetahui hubungan karakteristik klinikopatologi squamous cell carcinoma(SCC) serviks dengan TAN di RSUP Dr. Mohammad Hoesin Palembang.MetodeJenis penelitian ini adalah analitik dengan metode cross-sectional di mana pengumpulan data ini menggunakan 37 arsip slidesquamous cell carcinoma serviks di Bagian Patologi Anatomik RSUP Dr. Mohammad Hoesin Palembang pada periode 2017-2019.HasilDari 37 sampel penelitian, didapatkan karakteristik klinikopatologik mayoritas pasien SCC serviks terjadi pada kelompok usia 51tahun (51,4%), dengan jenis histopatologi non-keratinizing SCC sebanyak (75,7%) dan derajat diferensiasi tersering yaitumoderately differentiated (75,7%). Kepadatan TAN banyak didapatkan pada kepadatan rendah (73%). Terdapat hubungan yangsignifikan antara derajat diferensiasi dan kepadatan TAN (p=0,035) dan tidak terdapat hubungan yang signifikan antara usia dankepadatan TAN (p=0,269).KesimpulanSemakin tinggi derajat diferensiasi, maka nilai kepadatan semakin rendah. Namun masih perlu dibuktikan apakah neutrofil padapenelitian ini merupakan neutrofil yang anti-tumor (N1) atau pro-tumor (N2). Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/501 10.55816/mpi.v31i2.501 Majalah Patologi Indonesia; Vol. 31 No. 2, Mei 2022; 417 - 421 2527-9106 0215-7284 10.55816/mpi.v31i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/501/383 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/502 2025-12-19T17:08:06Z patologi:ART driver

Histopathologic Factors Affecting Metastases to Lymph Nodes in Laryngeal Squamous Cell Carcinoma in the Department of Anatomical Pathology FKUI/RSCM 2015-2019 Faktor-faktor Histopatologik yang Memengaruhi Metastasis ke Kelenjar Getah Bening pada Karsinoma Sel Skuamosa Laring di Departemen Patologi Anatomik FKUI/RSCM Tahun 2015-2019 Laelasari, Ela Lisnawati laryngeal squamous cell carcinoma, lymph node metastases, histopathologic. karsinoma sel skuamosa laring, metastasis ke KGB, histopatologik. BackgroundLaryngeal squamous cell carcinoma (LSCC) is the second most common epithelial carcinoma of the head and neck. This carcinomahas a poor prognosis if there have been metastases in the neck lymph nodes and did not get proper therapheutical management.The aims of the study is to determine the histopathologic characteristics of LSCC with partial or total laryngectomy and neckdissection, as well as its relationship with the incidence of metastases to lymph nodes.MethodsAnalytical descriptive study with cross-sectional design of secondary data on LSCC cases obtained through total or partiallaryngectomy accompanied by neck dissection from the archives of the Department of Anatomic Pathology FKUI / RSCM fromJanuary 2015 to December 2019. The histopathological data obtained were analyzed by comparative statistical test.ResultsKSSL was more frequently found in men as much as 155 cases (94.5%) with the most common age was over 60 years old. In thisstudy, LSCC with lymph nodes metastases was found in 65 cases, the most common was tumor size >4 cm in 40 cases (61.5%),medium differentiated tumors in 47 cases (72.3%), pT3-pT4 in 57 cases. (87.7%). There was a significant association betweenmetastasis to cervical lymph nodes with tumor size, poorly differentiated tumors, lymphovascular invasion and pT3-pT4.ConclusionHistopathologic factors including tumor size, pT3-pT4, poor differentiation, lymphovascular invasion are factors that play a role in theoccurrence of metastases to cervical lymph nodes in LSCC Latar belakangKarsinoma sel skuamosa laring (KSSL) merupakan karsinoma epitelial tersering kedua pada daerah kepala dan leher. Karsinomaini memiliki prognosis yang buruk bila telah ditemukan metastasis ke kelenjar getah bening (KGB) leher dan tidak mendapatkanpenatalaksanaan secara tepat. Tujuan penelitian ini untuk mengetahui karakter histopatologik pada KSSL dengan laringektomiparsial maupun total dan diseksi leher, serta hubungannya dengan kejadian metastasis ke kelenjar getah bening (KGB).MetodePenelitian deskriptif analitik dengan desain potong lintang pada data sekunder kasus KSSL yang ditemukan melalui laringektomitotal atau parsial disertai diseksi leher dari arsip Departemen Patologi Anatomik FKUI/RSCM Januari 2015 sampai denganDesember 2019. Data histopatologik yang ditemukan dianalisis dengan uji statistik komparatif.HasilKSSL lebih sering ditemukan pada laki-laki sebanyak 155 kasus (94,5%) dengan usia tersering >60 tahun. Pada penelitian ini,KSSL dengan metastasis ke KGB ditemukan pada 65 kasus, tersering yaitu ukuran tumor >4 cm sebanyak 40 kasus (61,5%), tumorberdiferensiasi sedang sebanyak 47 kasus (72,3%), pT3-pT4 sebanyak 57 kasus (87,7%). Terdapat hubungan bermakna antarametastasis ke KGB leher dengan ukuran tumor, tumor berdiferensiasi buruk, invasi limfovaskular dan pT3-pT4.KesimpulanFaktor-faktor histopatologik yaitu ukuran tumor, pT3-pT4, diferensiasi buruk, invasi limfovaskular merupakan faktor-faktor yangberperan pada terjadinya metastasis ke KGB leher pada KSSL. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/502 10.55816/mpi.v31i2.502 Majalah Patologi Indonesia; Vol. 31 No. 2, Mei 2022; 422 - 429 2527-9106 0215-7284 10.55816/mpi.v31i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/502/384 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/503 2025-12-19T17:08:06Z patologi:ART driver

Unexpected Case of Cerebral Toxoplasmosis with Radiological Appearance Similar to Malignancy Kasus Toksoplasmosis Serebri Tak Terduga dengan Gambaran Radiologi yang Menyerupai Keganasan Sekarputri, Cempaka Harsa Indiralia, Anisia Roebijanti toksoplasmosis, cerebri, HIV, brain biopsy toksoplasmosis, serebral, HIV, biopsi otak ABSTRACTToxoplasmosis was Toxoplasma Gondii infection. In HIV-infected patients, the prevalence of toxoplasma infectionranges was 3-97%. Diagnosis cerebral toxoplasmosis almost failed to recognized varians clinical and radiologicalfeatures on organism identifiied from brain biopsy which difficult to extract from alive patient. This case reportdiscussed cerebral toxoplasmosis of brain biopsy HIV positive patient which unknown status HIV patient, previously.A 52-year-old male patient came complained not only loss of apatit and dizzy two months ago, but also fainted.MSCT Scan with and without contrast showed right temporooccipital glioblastoma. Brain MRI showed a lesion on theright temporoparietal area with perifocal edema pressing on the right lateral ventricle. Microscopical feature showedglial tissue containing large areas of necrosis with karyorrhexis debris, PMN and mononuclear leucocyte infiltration,scaterred of tachyzoites and bradyzoites, no signs of malignancy. HIV serological examination was confirmed and thepatient's results were found to be anti-HIV reactive. Conclusion diagnosis as cerebral toxoplasmosis. ABSTRAKToksoplasmosis merupakan infeksi oleh Toxoplasma Gondii. Prevalensi infeksi toksoplasma pada pasien terinfeksiHIV adalah 3-97%. Diagnosis toksoplasmosis serebral sering terlewat karena kegagalan mengenali berbagaipresentasi klinis dan gambaran radiologi, terhadap hasil identifikasi organisme yang berasal dari biopsi otak, yangsukar dilakukan pada saat pasien masih hidup. Laporan kasus ini membahas toksoplasmosis serebral, yang berupabahan biopsi otak pasien HIV positif yang sebelumnya status HIV pasien tidak diketahui. Pasien laki-laki 52 tahun,datang ke RS dengan keluhan tidak mau makan dan pasien merasa pusing sejak dua bulan lalu, juga pasien sempatpingsan. Hasil MSCT Scan kepala dengan dan tanpa kontras menunjukkan gambaran Glioblastoma temporooksipital kanan. Hasil MRI Brain, lesi di temporoparietal kanan disertai perifokal edema mendesak ventrikel lateraliskanan. Pemeriksaan mikroskopik otak menunjukkan jaringan glia mengandung area nekrosis luas disertai debriskaryoreksis, sebukan sel-sel lekosit PMN dan sel-sel mononukleus; sebaran takizoit dan bradizoit; tanpa tanda-tandakeganasan. Pada pemeriksaan serologi HIV ditemukan anti-HIV reaktif. Kesimpulan diagnosis adalah toksoplasmosisserebral. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/503 10.55816/mpi.v31i2.503 Majalah Patologi Indonesia; Vol. 31 No. 2, Mei 2022; 430 - 435 2527-9106 0215-7284 10.55816/mpi.v31i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/503/385 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/504 2025-12-19T17:08:06Z patologi:ART driver

Characteristics of Cervical Precancerous Lesions at Tertiary Hospital in West Java, Indonesia Karakteristik Pasien Lesi Prakanker Serviks di Rumah Sakit Tersier Jawa Barat, Indonesia Maulana, Akbar Dewayani, Birgitta Maria Hernowo, Bethy Surjawathy cervical intraepithelial neoplasia, cervical cancer, characteristic, risk factor : faktor risiko, karakteristik, neoplasia intraepitel serviks BackgroundThe incidence of cervical cancer was continues to increase even though various risk factors and prevention efforts have been made.Cervical cancer begins with the development of a precancerous lesion (Cervical Intraepithelial Neoplasia/CIN) which is an abnormalcell growth in the cervical epithelium. This study aims to see the proportion of risk factors for cervical cancer in CIN patients at Dr.Hasan Sadikin, Bandung.MethodsThis study used a cross-sectional retrospective descriptive research method using secondary data of CIN patients for the periodJanuary 2016 - December 2020 from the archives of the anatomical pathology laboratory and clinical medical records of patients inthe form of age, marriage history, total parity, age at marriage, age at menarche, usage of hormonal contraceptives, smoking habits,clinical symptoms and histopathological degrees.ResultThere were total sample 40 cases of CIN. The 41-50 year age category is the largest age range of all NIS cases (35%). The resultsof this research data were history of marriage 1x (85%), age at marriage in the range of 20-30 years (47.5%), history of menarche inthe age range 10-15 years (90%), history of multiparous (65%), used of hormonal contraceptives (52.5%), non-smoking habits(67.5%), clinical symptoms of vaginal bleeding (42.5%) and histopathological grade of CIN 1 (47.5%).ConclusionCervical precancerous lesions at RSUP Dr. Hasan Sadikin occurred between fourth and fifth decades of life with a history of beingmarried once, multiparous, married at the age of 20-30 years, menarche at the age of 10-15 years, using hormonal contraception,not smoking, complaints of vaginal bleeding and the majority of cases were CIN 1. Latar belakangAngka kejadian kanker serviks terus meningkat walaupun berbagai faktor risiko dan usaha pencegahan telah dilakukan. Kankerserviks berawal dari perkembangan lesi prakanker (Neoplasia Intraepitelial Serviks/NIS) yang merupakan pertumbuhan selabnormal pada epitel serviks. Penelitian ini bertujuan untuk melihat proporsi faktor-faktor risiko terjadinya kanker serviks padapasien NIS di RSUP Dr. Hasan Sadikin, Bandung.MetodeStudi ini menggunakan metode penelitian deskriptif retrospektif secara potong lintang dengan menggunakan data sekunder pasienNIS periode Januari 2016-Desember 2020 dari arsip laboratorium patologi anatomik dan rekam medis klinis pasien berupa usia,riwayat pernikahan, jumlah paritas, usia saat menikah, usia saat menarche, penggunaan alat kontrasepsi, riwayat merokok, gejalaklinis dan derajat histopatologi.HasilTerdapat total sampel NIS sebanyak 40 kasus. Kategori usia 41-50 tahun merupakan rentang usia terbanyak dari seluruh kasusNIS (35%). Hasil data penelitian ini antara lain: riwayat pernikahan satu kali (85%), usia saat menikah pada rentang 20-30 tahun(47,5%), riwayat menarche pada rentang usia 10-15 tahun (90%), riwayat multipara (65%), penggunaan kontrasepsi hormonal(52,5%), kebiasaan tidak merokok (67,5%), gejala klinis perdarahan pervaginam (42,5%) dan derajat histopatologi NIS 1 (47,5%).KesimpulanLesi prakanker serviks di RSUP Dr. Hasan Sadikin terjadi pada usia dekade keempat dan kelima dengan riwayat menikah satu kali,multipara, menikah pada usia 20-30 tahun, menarche pada usia 10-15 tahun, menggunakan kontrasepsi hormonal, tidak merokok,keluhan perdarahan pervaginam dan mayoritas kasus adalah NIS 1 Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/504 10.55816/mpi.v31i2.504 Majalah Patologi Indonesia; Vol. 31 No. 2, Mei 2022; 436 - 444 2527-9106 0215-7284 10.55816/mpi.v31i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/504/386 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/505 2025-12-19T17:08:06Z patologi:ART driver

Diagnostic Accuracy and Cytomorphological Analysis of Biliary Brush and Endoscopic Ultrasound Guided Fine Needle Aspiration (EUS-FNA) of the pancreas based on The Papanicolaou Society of Cytopathology System Akurasi Diagnostik dan Analisis Gambaran Sitomorfologi Sikatan Bilier dan Endoscopic Ultrasound Guided Fine Needle Aspiration (EUS-FNA) Pankreas Berdasarkan The Papanicolaou Society of Cytopathology System Sarwanti Stephanie, Marini Biliary brush cytology, Endoscopic ultrasound guided fine needle aspiration (EUS-FNA), sensitivity, specificity, accuracy Sitologi sikatan bilier, Endoscopic ultrasound guided fine needle aspiration (EUS-FNA), sensitivitas, spesifisitas, akurasi. BackgroundEndoscopic ultrasound guided fine needle aspiration (EUS-FNA) of the pancreas and biliary brushes is a minimally invasive method ofcytology sampling. The purpose of this study was to determine the sensitivity, specificity, and accuracy of examination of biliary brushcytology and EUS-FNA pancreas based on the System of standardized terminology and nomenclature for pancreatobilliary cytology(STNPC) as well as cytology characteristics of pancreatic malignancy.MethodsA search of the biliary brush preparation and EUS-FNA pancreatic in 2017-2020 was carried out in the Archives section of the Department ofAnatomical Pathology FKUI / RSCM. 506 cases of biliary brush cytology and EUS-FNA pancreatic, conducted a search of histopathologicalpreparations and re-categorized based on STNPC.ResultsCytological cases of biliary brushing and pancreatic EUS-FNA paired with histopathology in 2017-2020 151 cases, 85 cases of biliarybrushing and 66 cases of pancreatic EUS-FNA, were re-categorized based on STNPC with results of biliary brushing 27 non-diagnosticcases, 24 atypical cases, 7 suspicious for malignancy (SFM) cases and 17 malignant cases. Meanwhile, for pancreatic EUS-FNA cytology16 non-diagnostic cases, 2 negative cases, 16 atypical cases, 3 other neoplasms, 4 SFM cases and 25 malignant cases. Twenty sevencases of false negative biliary brushes on non-diagnostic and atypical preparations and 4 cases of false negative on pancreatic EUS-FNA onnon-diagnostic and atypical preparations. Biliary brushing diagnostic test results: sensitivity 82.35%, specificity 100%, positive predictivevalue (PPV) 100%, negative predictive value (NPV) 84.21% and accuracy 90.9%. In pancreatic EUS-FNA: sensitivity 84.21%, specificity100%, PPV 100%, NPV 986.67% and accuracy 92.86%.ConclusionCytological diagnosis of biliary brushing and pancreatic EUS-FNA has good specificity, sensitivity and accuracy, but definitive diagnosisrequires histopathological examination ABSTRAKLatar belakangEndoscopic ultrasound guided fine needle aspiration (EUS-FNA) pankreas dan sikatan bilier merupakan cara pengambilan sampel sitologiyang minimal invasif. Tujuan penelitian ini untuk mengetahui sensitivitas, spesifisitas, dan akurasi pemeriksaan sitologi sikatan bilier danEUS-FNA pankreas berdasarkan System of standardized terminology and nomenclature for pancreatobilliary cytology (STNPC) sertakarakteristik sitologi keganasan pankreas.MetodeDilakukan penelusuran sediaan sikatan bilier dan EUS-FNA pankreas tahun 2017-2020 di bagian Arsip Departemen Patologi AnatomikFKUI/RSCM. 506 kasus sitologi sikatan bilier dan EUS-FNA pankreas, dilakukan penelusuran sediaan histopatologiknya dan dibuat kategoriulang berdasarkan STNPC.HasilKasus sitologi sikatan bilier dan EUS-FNA pankreas berpasangan dengan histopatologi tahun 2017-2020 151 kasus, 85 kasus sikatan bilierdan 66 kasus EUS-FNA pankreas, dilakukan kategori ulang berdasarkan STNPC dengan hasil sikatan bilier 27 kasus non diagnostik, 24kasus atipik, 7 kasus suspicious for malignancy (SFM) dan 17 kasus ganas. Sedangkan untuk sitologi EUS-FNA pankreas 16 kasus nondiagnostik, 2 kasus negatif, 16 kasus atipik, 3 kasus neoplasma lainnya, 4 kasus SFM dan 25 kasus ganas. 27 kasus sikatan bilier negatifpalsu pada sediaan non diagnostik dan atipik dan 4 kasus negatif palsu pada EUS-FNA pankreas pada sediaan non diagnostik dan atipik.Hasil uji diagnostik sikatan bilier: sensitivitas 82,35%, spesifisitas 100%, positive predictive value (PPV) 100%, negative predictive value(NPV) 84,21% dan akurasi 90,9%. Pada EUS-FNA pankreas: sensitivitas 84,21%, spesifisitas 100%, PPV 100%, NPV 986,67% dan akurasi92,86%.KesimpulanDiagnosis sitologi sikatan bilier dan EUS-FNA pankres memiliki spesifisitas, sensitivitas dan akurasi yang baik namun diagnosis definitifmemerlukan pemeriksaan histopatologik Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/505 10.55816/mpi.v31i2.505 Majalah Patologi Indonesia; Vol. 31 No. 2, Mei 2022; 445 - 454 2527-9106 0215-7284 10.55816/mpi.v31i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/505/387 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/506 2025-12-19T17:08:06Z patologi:ART driver

Association between Clinicopathological Profile and Recurrence of Invasive Breast Carcinoma in Anatomical Pathology FKUI/RSCM Hubungan Profil Klinikopatologik dengan Rekurensi Karsinoma Payudara Invasif di Departemen Patologi Anatomik FKUI/RSCM Anggreani, Ineke Rustamadji, Primariadewi invasive breast carcinoma, clinicopathology, therapy, recurrence. : karsinoma payudara invasif, klinikopatologik, terapi, rekurensi. ABSTRACTBackgroundBreast cancer is the most common malignancy and the leading cause of death in women. Invasive breast carcinoma is a breastmalignancy originating from the glandular epithelium of the breast. Approximately 40% of invasive breast carcinomas had recur.There are several factors that influence the occurrence of recurrence, such as age, tumor size, lymphovascular invasion, incisionmargins, hormonal status and therapy. This study aims to determine the clinicopathological profile associated with recurrence 5years after therapy for invasive breast carcinoma.MethodsThis study is a retrospective study with data collection carried out through the archives of the Department of Anatomical Pathology,Faculty of Medicine, University of Indonesia, Dr. Cipto Mangunkusumo General Hospital within a period of 2 years (2014-2015). Theclinicopathological data was searched for up to 5 years of therapy.ResultsFrom the 54 cases of invasive breast carcinoma in this study, all were women with mean age 48.3 years. A total of 18 cases(33.3%) experienced recurrence, especially in patients aged ≤50 years, tumor mass size more than 2 cm and luminal B moleculartype. Tumor margin is difficult to evaluate because many review cases do not submit the tumor margin section. Therapy received bypatients was in accordance with standard procedures’ hospital, both neoadjuvant and adjuvant chemotherapy. From the statisticalanalysis, there was no significant relation between all profiles that studied with recurrence. It is because the comparison of numberof recurrent and non-recurrent cases is not equal.ConclusionOne third of advanced invasive breast carcinomas treated with neoadjuvant and showing a partial response have recurrence,whereas no complete response has recurrence. Tumor size, histologic grade, molecular type and adherence to routine follow-uptreatment influenced the recurrence within 5 years of treatment. ABSTRAKLatar belakangKanker payudara merupakan keganasan tersering dan penyebab kematian tertinggi pada wanita. Karsinoma payudara invasifmerupakan keganasan payudara yang berasal dari epitel kelenjar payudara. Sekitar 40% karsinoma payudara invasif mengalamirekurensi. Terdapat beberapa faktor yang mempengaruhi terjadinya rekurensi, seperti usia, ukuran tumor, invasi limfovaskular,batas sayatan, status hormonal dan terapi yang diberikan. Penelitian ini bertujuan untuk mengetahui profil klinikopatologikberhubungan dengan rekurensi 5 tahun pasca terapi pada karsinoma payudara invasif.MetodePenelitian ini merupakan penelitian retrospektif dengan pengumpulan data dilakukan melalui arsip Departemen Patologi AnatomikFakultas Kedokteran Universitas Indonesia/RSUPN Dr. Cipto Mangunkusumo (FKUI/RSCM) dalam kurun waktu 2 tahun (2014-2015). Dilakukan penelusuran data klinikopatologik sampai 5 tahun terapi.HasilDari 54 kasus karsinoma payudara invasif penelitian ini, seluruhnya perempuan dengan usia rata-rata 48,3 tahun. Terdapat 18kasus (33,3% kasus) mengalami rekurensi, terutama pada pasien usia ≤50 tahun, ukuran massa tumor lebih dari 2 cm dan tipemolekular luminal B. Batas sayatan sulit dievaluasi karena banyak sediaan review yang tidak mengirimkan batas sayatan. Terapiyang didapatkan pasien sesuai dengan standar prosedur rumah sakit, baik yang didahului neoadjuvan sebelum operasi maupuntidak didahului neoadjuvan. Dari hasil analisis statistik, tidak ditemukan hubungan yang bermakna dari seluruh profil yang ditelitidengan rekurensi. Hal ini dikarenakan perbandingan jumlah kasus rekuren dan tidak rekuren tidak sebanding.KesimpulanSepertiga karsinoma payudara invasif stadium lanjut yang diterapi neoadjuvan dan menunjukkan respon parsial mengalamirekurensi, sedangkan respon komplit tidak ada yang rekuren. Ukuran tumor, derajat histologik, tipe molekular dan pengobatanmempengaruhi terjadinya rekurensi dalam 5 tahun Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/506 10.55816/mpi.v31i2.506 Majalah Patologi Indonesia; Vol. 31 No. 2, Mei 2022; 455 - 461 2527-9106 0215-7284 10.55816/mpi.v31i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/506/388 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/512 2025-12-19T17:06:28Z patologi:ART driver

Clinicopathological Profile of Prostate Adenocarcinoma Cases at Cipto Mangunkusumo Hospital in 2010-2019 with Special Overview of Robotic Biopsy Profil Klinikopatologik Kasus Adenokarsinoma Prostat di RSCM Tahun 2010-2019 dengan Tinjauan Khusus Biopsi Robotik Rizky Prasetya, Gusti Saraswati, Meilania Rizal AH. Hamid, Agus BRE. Matondang, Sahat prostate adenocarcinoma, WHO/ISUP grade, biopsy, robotic technique, PIRADS adenokarsinoma prostat, derajat WHO/ISUP, biopsi, teknik robotik, PIRADS. BackgroundProstate adenocarcinoma is second most common malignancy in men and fourth leading death cause from malignancy worldwide. Prostate biopsy is the gold standard for diagnosing this disease. However, the difficulty of sampling is a weakness in this procedure. In Cipto Mangunkusumo hospital, biopsy technology is now available with robotic techniques for biopsy sampling, which is expected to increase prostate biopsy accuracy. This study was to determine prostate adenocarcinoma case profile in the Department of Pathology Anatomic Cipto Mangunkusumo Hospital for 10 years and to examine differences in sampling of biopsy preparations with robotic and non-robotic techniques. MethodsThis study was an exploratory descriptive study with cross-sectional design to determine prostate adenocarcinoma case profile in the Department of Anatomic Pathology FKUI-RSCM for 10 years. The data obtained between the Gleason score of prostate biopsy preparations and surgery. Robotic prostate biopsy results were compared with prostate imaging reporting and data system (PIRADS) score. ResultsThe mean age of the patients was 67 years old and several patients were aged <50 years old. Many poor differentiation cases were found. It appears that there is a relationship between WHO/ISUP degree and perineural invasion. In 14 patients who underwent histopathological examination more than once, it was found that 9 patients had the same WHO/ISUP degree and 5 patients had a changed WHO/ISUP degree. There are several discrepancies in PIRADS score and histopathological findings from 21 robotic preparations examined. ConclusionThe mean age of patients fit to theory of generalized prostate adenocarcinoma. Many cases of poor differentiation were found, possibly related to RSCM as a national referral center. The repeated sample examination results are similar to the results of other studies. Robotic method biopsy gives the sense of more comprehensive sampling in comparison to non-robotic method. Latar belakangAdenokarsinoma prostat merupakan keganasan yang ditemukan tersering kedua pada laki-laki dan peringkat keempat penyebab kematian akibat keganasan di seluruh dunia. Biopsi prostat merupakan baku emas untuk diagnosis penyakit, namun jumlah sampling yang adekuat serta pemetaan area prostat yang mengandung lesi merupakan suatu diskusi tersendiri. Saat ini, di Rumah Sakit Cipto Mangunkusumo (RSCM) tersedia teknologi biopsi dengan teknik robotik yang diharapkan meningkatkan ketepatan diagnosis serta pemetaan lesi di prostat.Tujuan penelitian ini untuk mengetahui profil kasus adenokarsinoma prostat di Departemen Patologi Anatomik RSCM selama 10 tahun, serta mengkaji perbedaan sampling sediaan biopsi dengan teknik robotik dan non-robotik.MetodePenelitian ini adalah penelitian deskriptif eksploratif potong lintang untuk mengetahui profil kasus adenokarsinoma prostat di Departemen Patologi Anatomik FKUI-RSCM selama 10 tahun. Dilakukan eksplorasi khusus pada biopsi prostat robotik yang pengambilan lesi menggunakan bantuan radiologi yaitu prostate imaging reporting and data system (PIRADS). HasilDilakukan evaluasi terhadap 405 kasus biopsi prostat. Rata-rata usia pasien adalah 67 tahun dan terdapat beberapa pasien berusia <50 tahun. Terdapat hubungan antara derajat WHO/ISUP dengan invasi perineural. Pada 14 pasien yang menjalani pemeriksaan histopatologilebih dari satu kali didapatkan 9 pasien memiliki derajat WHO/ISUP yang sama dan 5 pasien derajat WHO/ISUP berubah. Dari 21 sediaan robotik yang dieksplorasi, terdapat beberapa ketidaksesuaian skor PIRADS dan diagnosis histopatologi.KesimpulanRata-rata usia pasien sesuai dengan teori pada adenokarsinoma prostat umumnya. Kasus diferensiasi buruk banyak ditemukan kemungkinan berhubungan dengan RSCM sebagai pusat rujukan nasional. Hasil pemeriksaan spesimen yang berulang serupa dengan hasil penelitian lain. Biopsi robotik memberikan kesan cakupan pengambilan sampel spesimen yang lebih komprehensif dibandingkan metode non-robotik dengan pemetaan area lesi di prostat yang dapat membantu penentuan staging yang lebih baik. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-09-14 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/512 10.55816/mpi.v31i3.512 Majalah Patologi Indonesia; Vol 31. No. 3, September 2022 2527-9106 0215-7284 10.55816/mpi.v31i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/512/373 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/515 2025-12-19T17:06:28Z patologi:ART driver

The Correlation of E-cadherin Expression with Tumor Gradeand Tumor Budding in Endometrioid Endometrial Carcinoma Hubungan Ekspresi E-cadherin dengan Derajat Histopatologik dan Tumor Budding pada Karsinoma Endometrium Jenis Endometrioid Pemuda, Haris Asri, Aswiyanti Zuryati Nizar, Rachmadijah Yetti, Husna endometrioid endometrial carcinoma, E-cadherin expression, tumor grade, tumor budding. karsinoma endometrium jenis endometrioid, ekspresi E-cadherin, derajat diferensiasi, tumor budding. BackgroundEndometrial carcinoma is the fourth most common malignancy among women worldwide with increasing incidence and death rate every year. One of the types of endometrial carcinoma is endometrioid carcinoma, originated from atypical hyperplasia and develop into carcinoma. Lack of intercellular cohesiveness in the epithelial tumors such as endometrioid carcinoma occur due to lack of expression of E-cadherin. It can also causetumor invasion and metastatic through the epithelial-mesenchymal transition (EMT) process. Tumor differentiation and tumor budding are presumed to be histopathologic representations due to lack of cohesiveness and the occurrence of the EMT process so that these two things need to be related with the expression of E-cadherin on tumor cells.MethodsAn observational study was conducted using a cross-sectional approach with 46 cases of endometrioid endometrial carcinoma.Samples were obtained from 4 Anatomical Pathology Laboratories in West Sumatra during 2016-2019 in the form of paraffin blocks and HE slides that obtained from hysterectomy surgery. Reevaluation of tumor grade and tumor budding in HE slides was performed. E-cadherin expression in tumor cells was seen by immunohistochemical staining. Bivariate statistical analysis was performed using Fisher's Exact test and the results were considered significant if the p value was <0.05.ResultsSpecimens with negative E-cadherin expression were more common in grade 2 tumors (41.7%) and grade 3 tumors (50.0%) and in specimes with positive tumor budding (91.7%). Statistical analysis revealed a significant correlation between E-cadherin expression with tumor grade (p=0.000) and tumor budding (p=0.000).ConclusionExpression of E-cadherin has a significant correlation with the tumor grade and tumor budding in endometrioid endometrial carcinoma. Latar belakangKarsinoma endometrium adalah keganasan keempat tersering pada wanita di dunia dengan peningkatan angka kejadian dan kematian setiap tahunnya. Salah satu jenis karsinoma endometrium adalah karsinoma endometrioid yang berasal dari hiperplasia atipikal dan berkembang menjadi karsinoma. Berkurangnya daya kohesif antar sel pada tumor epitelial seperti karsinoma endometrioid dapat terjadi karena berkurangnya ekspresi E-cadherin. Penurunan ekspresi E-cadherin juga menyebabkan invasi dan metastasis tumor melalui proses epithelial-mesenchymal transition (EMT). Derajat histopatologik dan tumor budding diduga merupakan representasi histopatologik akibat berkurangnya daya kohesif dan terjadinya proses EMT sehingga kedua hal ini perludihubungkan dengan ekspresi E-cadherin pada sel-sel tumor.MetodePenelitian observasional melalui pendekatan cross sectional dilakukan dengan jumlah sampel sebanyak 46 kasus karsinoma endometrium jenis endometrioid. Sampel diperoleh dari 4 Laboratorium Patologi Anatomik di Sumatera Barat selama periode tahun 2016-2019 berupa blok parafin dan slaid HE yang dibuat dari jaringan pasien hasil tindakan histerektomi. Reevaluasi dilakukan terhadap derajat diferensiasi dan tumor budding. Ekspresi E-cadherin pada sel tumor dilihat dengan pewarnaan imunohistokimia. Analisis statistik bivariat dilakukan dengan uji Fisher's Exact dan hasil dianggap bermakna jika nilai p<0,05.HasilSampel dengan ekspresi E-cadherin negatif lebih banyak ditemukan pada tumor derajat 2 (41,7%) dan tumor derajat 3 (50,0%) serta pada sampel dengan gambaran tumor budding positif (91,7%). Analisis statistik menunjukkan hubungan bermakna antara ekspresi E-cadherin dengan derajat diferensiasi (p=0,000) dan tumor budding (p=0,000).KesimpulanEkspresi E-cadherin mempunyai hubungan yang bermakna dengan derajat diferensiasi dan tumor budding pada karsinoma endometrium jenis endometrioid. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-09-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/515 10.55816/mpi.v31i3.515 Majalah Patologi Indonesia; Vol 31. No. 3, September 2022 2527-9106 0215-7284 10.55816/mpi.v31i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/515/374 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/516 2025-12-19T17:06:28Z patologi:ART driver

Relationship between Age and Gender with Histopathological Type of Nasopharyngeal Carcinoma Hubungan antara Usia dan Jenis Kelamin dengan Tipe Histopatologi Karsinoma Nasofaring Melvern, Bryan Karlowee, Vega Dewi Kusuma Astuti, Meira nasopharyngeal carcinoma, incidence, age, sex, histopathological type karsinoma nasofaring, insidensi, usia, jenis kelamin, tipe histopatologi BackgroundNasopharyngeal carcinoma (NPC) is a malignancy with a high incidence in Indonesia. Recent studies had found several factors that influence the incidence and prognosis of NPC including age, sex, and histopathological type. This study aimed to determine the relationship between age and sex with the histopathological type of NPC.MethodsSubjects were NPC patients diagnosed by the Anatomical Pathology Department of Dr. Kariadi General Hospital Medical Center (RSUP Dr. Kariadi) from 1st January 2017-31st December 2019. Data were collected from the medical record. The relationship between age and sex with the histopathological type of NPC was examined. ResultsThere were 201 NPC patients during 2017-2019 in RSUP Dr. Kariadi. The highest number of NPC incidence was in the range of 54-59 years old. There were more males than females at the ratio of 2.24:1. Undifferentiated non-keratinizing cell carcinoma type NPC was the most common type (90.5% of NPC patients), followed by differentiated non-keratinizing cell carcinoma and squamous cell carcinoma consecutively. Both age and sex had no significant relationship with the histopathological type of NPC (p>0.05).ConclusionThere was no relationship between age and sex with the histopathological type of NPC. Latar belakangKarsinoma nasofaring merupakan keganasan dengan insidensi yang tinggi di Indonesia. Penelitian terbaru menemukan beberapa faktor yang memengaruhi insidensi dan prognosis karsinoma nasofaring termasuk usia, jenis kelamin, dan tipe histopatologi. Penelitian ini bertujuan untuk mengetahui hubungan antara usia dan jenis kelamin terhadap tipe histopatologi karsinoma nasofaring.MetodeSubjek dalam penelitian ini meliputi pasien karsinoma nasofaring yang didiagnosis oleh Bagian Patologi Anatomik Rumah Sakit Umum Pusat Dr. Kariadi (RSUP Dr. Kariadi) sejak 1 Januari 2017-31 Desember 2019. Data didapatkan dari rekam medis. Hubungan antara usia dan jenis kelamin dengan tipe histopatologi karsinoma nasofaring telah dinilai.HasilTerdapat 201 pasien karsinoma nasofaring di RSUP Dr. Kariadi sejak tahun 2017-2019. Insidensi tertinggi terdapat pada kelompok usia 54-59 tahun. Didapatkan lebih banyak pasien berjenis kelamin laki laki dibanding perempuan dengan rasio 2,24:1. Tipe undifferentiated non-keratinizing cell carcinoma merupakan tipe paling umum (90,5% dari seluruh pasien karsinoma nasofaring), diikuti dengan differentiated non-keratinizing cell carcinoma dan squamous cell carcinoma secara berurutan. Baik usia maupun jenis kelamin tidak memiliki hubungan yang signifikan terhadap tipe histopatologi karsinoma nasofaring (p>0,05).KesimpulanTidak terdapat hubungan yang signifikan antara usia dan jenis kelamin terhadap tipe histopatologi karsinoma nasofaring. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-09-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/516 10.55816/mpi.v31i3.516 Majalah Patologi Indonesia; Vol 31. No. 3, September 2022 2527-9106 0215-7284 10.55816/mpi.v31i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/516/375 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/517 2025-12-19T17:06:28Z patologi:ART driver

Diagnostic Accuracy and Analysis of Cytomorphological Images of Fine Needle Aspiration Biopsy in Salivary Gland Lesions Based on The Milan System for Reporting Salivary Gland Cytology (MSRSGC) Classification Akurasi Diagnostik dan Analisis Gambaran Sitomorfologi Biopsi Aspirasi Jarum Halus pada Lesi Kelenjar Liur Berdasarkan Klasifikasi The Milan System for Reporting Salivary Gland Cytology (MSRSGC) Fossetta Manatar, Amelia Lisnawati diagnostic accuracy, fine needle aspiration cytology, MSRSGC, salivary gland lesion akurasi diagnostik, lesi kelenjar liur, MSRSGC, sitologi aspirasi jarum halus BackgroundFine needle aspiration cytology (FNAC) is an effective, simple, and safe method of assessing salivary glandular lesions. The diagnosis of FNAC can be challenging for pathologists because of the heterogeneous morphological spectrum and overlapping of cytomorphologic salivary glands. The aim is to determine the diagnostic accuracy and analyze the cytomorphology of salivary glands aspiration based on The Milan System for Reporting Salivary Gland Cytology (MSRSGC). Methods A search for cytological preparations for salivary gland lesions in 2015-2019 was carried out in the archives of the Department of Anatomic Pathology, FKUI/RSCM. There were 215 cases of cytology of salivary gland lesions which re-evaluated and classified based on MSRSGC. There were 106 cases of cytologic-histopathologic paired data, 25 of which were excluded because the specimens were not filled the criteria for re-examination. ResultsThere were 81 cases of salivary gland lesions since 2015-2019 which were assessed and reclassified based on MSRSGC, consisting of 8 non-diagnostic, 1 non-neoplastic, 1 case of atypical of undetermined significance (AUS), 39 benign tumors, 10 salivary gland neoplasm of undetermined malignant potential (SUMP), 6 suspicious for malignancy (SFM) and 16 malignant tumors. Three of these cases had a different diagnosis result in false-negative on non-diagnostic and benign tumors. The sensitivity value was 91.17%, specificity 97.43%, positive predictive value (PPV) 96.87%, negative predictive value (NPV) 92.68% and 94.52% accuracy. ConclusionThe diagnosis of fine needle aspiration cytology can be accurately achieved by a combination of clinical information and radiological examination, however a histopathologic examination is still required for definitive diganosis. MSRSGC classification is very helpful in the diagnosis of salivary gland cytology. Latar belakangBiopsi aspirasi jarum halus (BAJH) merupakan metode yang efektif, sederhana dan aman dalam menilai lesi kelejar liur. Gambaran morfologi yang bervariasi serta sitomorfologik yang saling tumpang tindih, mengakibatkan sulitnya dalam mendiagnosis lesi kelenjar liur. Akurasi diagnostik BAJH bisa menjadi tantangan tersendiri bagi ahli patologi karena spektrum morfologi kelenjar liur yang heterogen.Tujuanpenelitian ini untuk mengetahui akurasi diagnostik dan menganalisa gambaran sitomorfologik aspirasi lesi kelenjar liur berdasarkan The Milan System for Reporing Salivary Gland Cytology (MSRSGC).MetodeDilakukan penelusuran sediaan sitologik lesi kelenjar liur tahun 2015-2019 pada arsip Departemen Patologi Anatomik FKUI/RSCM. Didapatkan 215 kasus sitologi lesi kelenjar liur, kemudian dilakukan penelusuran sediaan histopatologiknya, dievaluasi kembali serta diklasifikasikan berdasarkan MSRSGC. Terdapat 106 kasus data berpasangan sitologik-histopatologik, 25 kasus di antaranya dieksklusikarena tidak memenuhi kriteria untuk pemeriksaan ulang.HasilKasus lesi kelenjar liur sejak tahun 2015-2019 berjumlah 81 kasus yang dilakukan penilaian dan diklasifikasi ulang berdasarkan MSRSGC,terdiri atas 8 non diagnostik, 1 non neoplastik, 1 atypical of undetermined significance (AUS), 39 tumor jinak, 10 salivary gland neoplasm of undetermined malignant potential (SUMP), 6 suspicious for malignancy (SFM) dan 16 tumor ganas. Tiga kasus di antaranya memiliki diagnosis berbeda yang memberikan hasil negatif palsu pada sediaan non diagnostik dan tumor jinak. Nilai sensitivitas 91,17%, spesifisitas 97,43%, positive predictive value (PPV) 96,87%, negative predictive value (NPV) 92,68% dan akurasi 94,52%. KesimpulanDiagnosis sitologi aspirasi jarum halus dapat secara akurat ditegakkan dengan kombinasi keterangan klinik dan pemeriksaan radiologik namun untuk diagnosis definitif masih memerlukan pemeriksaan histopatologik. Klasifikasi MSRSGC sangat membantu dalam penegakan diagnosis sitologi kelenjar liur Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-09-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/517 10.55816/mpi.v31i3.517 Majalah Patologi Indonesia; Vol 31. No. 3, September 2022 2527-9106 0215-7284 10.55816/mpi.v31i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/517/376 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/518 2025-12-19T17:06:28Z patologi:ART driver

Clinicopathological Features of Neoadjuvant Chemotherapy Response to Triple Negative Breast Cancer in Dr. Hasan Sadikin General Hospital Gambaran Klinikopatologi pada Respon Kemoterapi Neoadjuvan Triple Negative Breast Cancer di Rumah Sakit Umum Pusat Dr. Hasan Sadikin Savitri, Widya Surjawathy Hernowo, Bethy Suryanti, Sri triple negative breast cancer, TNBC, clinicopathology, response to neoadjuvant chemotherapy, FAC Triple Negative Breast Cancer, TNBC, klinikopatologi, respons kemoterapi neoadjuvan, FAC BackgroundTriple Negative Breast Cancer (TNBC) accounts for 9-17% of all breast cancer diagnoses. TNBC has a high proliferation index and a poor prognosis with a higher response rate to neoadjuvant chemotherapy, but does not correlate with Overall Survival (OS) due to chemoresistence in more than 50% of TNBC. Neoadjuvant chemotherapy with anthracycline (doxorubicin) based regimens in combination with fluorouracil and cyclophosphamide (FAC) is still an option for TNBC. The aim of this study was to determine association between clinicopathological profile and response to neoajuvan chemotherapy in TNBC at Dr. Hasan Sadikin Bandung which is a referral hospital in West Java.MethodsThis study used an analytic observational method with a cross-sectional design. The research sample consisted of 40 TNBC cases from January 2017 to December 2019 at Dr. Hasan Sadikin hospital. Data obtained from medical records and archives of the Anatomical Pathology Laboratory consist of clinical response to neoadjuvant chemotherapy, age, tumor size, lymph node metastases and distant metastases, clinical stage, type of histopathology and lymphovascular vessel invasion.ResultsIn 40 cases of TNBC, 20 cases responded and 20 cases did not respond to neoadjuvant chemotherapy. In the non-response group, there were 90% of cases with histopathologic type invasive carcinoma of no special type and 75% showed invasion of lymphovascular vessels. There were no significant differences in age, tumor size, lymphovascular vessel invasion, stage, or histopathological type in the response and non-response groups.ConclusionThere is no correlation between clinicopathological features and neoadjuvant chemotherapy response in the case of Triple Negative Breast Cancer (TNBC). This can be caused by other factors including high heterogeneous tumor microenvironment (TME). Latar belakangTriple Negative Breast Cancer (TNBC) menyumbang 9-17% dari seluruh diagnosis kanker payudara. TNBC memiliki indeks proliferasi tinggi dan prognosis buruk dengan tingkat respons yang lebih tinggi terhadap kemoterapi neoadjuvant, namun tidak berkorelasi dengan overall survival (OS) disebabkan kemoresisten pada lebih dari 50% TNBC. Kemoterapi neoadjuvan dengan rejimen berbasis anthracycline(doxorubicin) dalam kombinasi dengan fluorouracil dan cyclophosphamide (FAC) masih menjadi pilihan pada TNBC.Tujuan penelitian ini adalah untuk mengetahui hubungan profil klinikopatologi pasien dengan respons kemoterapi neoajuvan pada TNBC di RSUP Dr. Hasan Sadikin Bandung yang merupakan rumah sakit rujukan di Jawa Barat.MetodePenelitian ini menggunakan metode observasional analitik dengan rancangan cross-sectional. Sampel penelitian terdiri atas 40 kasus TNBC periode Januari 2017 sampai Desember 2019 di RSUP Dr. Hasan Sadikin. Data didapat dari rekam medis dan arsip Laboratorium Patologi Anatomi terdiri dari respon klinis terhadap kemoterapi neoadjuvan, usia, ukuran tumor, metastasis kelenjar getah bening dan metastasis jauh, stadium klinis, jenis histopatologi serta invasi pembuluh limfovaskular.HasilPada 40 kasus TNBC terdapat 20 kasus berespon dan 20 kasus tidak berrespon terhadap kemoterapi neoadjuvan. Pada kelompok non respon terdapat 90% kasus dengan jenis histopatologi Invasive Carcinoma of No Special Type dan 75% menunjukkan invasi pembuluh limfovaskular. Tidak terdapat perbedaan signifikan dalam usia, ukuran tumor, invasi pembuluh limfovaskular, stadium, atau jenis histopatologi pada kelompok respon dan non-respon.KesimpulanGambaran klinikopatologi dengan respon kemoterapi neoadjuvan pada kasus Triple Negative Breast Cancer (TNBC) dari subjek penelitiantidak ada hubungan. Hal ini disebabkan oleh faktor lain di antaranya Tumor microenvironment (TME) heterogen yang tinggi. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-09-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/518 10.55816/mpi.v31i3.518 Majalah Patologi Indonesia; Vol 31. No. 3, September 2022 2527-9106 0215-7284 10.55816/mpi.v31i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/518/377 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/519 2025-12-19T17:06:28Z patologi:ART driver

The Role of Vascular Endothelial Growth Factor (VEGF) in the Pathogenesis of the Placenta Accreta Spectrum Peran Vascular Endothelial Growth Factor (VEGF) dalam Patogenesis Spektrum Plasenta Akreta 1 Linggodigdo, Michelle Hellyanti, Tantri Kusmardi Placenta Accreta Spectrum, PAS, angiogenesis, VEGF, FIGO 2020 Placenta Accreta Spectrum, PAS, angiogenesis, VEGF, FIGO 202 Placenta accreta spectrum (PAS) is a cause of serious maternal morbidity and mortality. FIGO 2020 designated PAS into grade 1 (non-invasive), grade 2 (superficial invasion), grade 3A (deep invasion), grade 3D (deep invasion with disruption of the serosa), and grade 3E (deep invasion with adherence to extrauterine structures). Currently, the trophoblast cell in accreta spectrum is associated with similar characteristics to cancer. Both conditions can induce angiogenesis, marked by upregulation of vascular endothelial growth factor (VEGF). This resulted in the formation of extensive neovascularization. The secretion of VEGF is induced by hypoxia, which was related to risk factor of PAS occurrence; defect of the decidua due to endometrial tissue damage. Placenta Accreta Spectrum (PAS) merupakan salah satu penyakit yang mengakibatkan morbiditas dan mortalitas ibu yang serius. Pedoman FIGO 2020 membagi PAS menjadi grade 1 (non-invasif), grade 2 (invasi superfisial), grade 3A (invasi dalam tanpa kerusakan serosa uterus), grade 3D (invasi dalam disertai kerusakan serosa uterus), dan grade3E (invasi dalam disertai perlekatan pada organ sekitar uterus). Saat ini sifat trofoblas pada plasenta akreta dikaitkan dengan sifat yang mirip dengan kanker, yaitu kemampuan angiogenesis. Faktor risiko kondisi PAS adalah kondisi hipoksia akibat defek desidua. Hal ini dapat memicu sekresi vascular endothelial growth factor (VEGF). Peningkatan VEGF berperan dalam pembentukan neovaskularisasi yang ekstensif pada PAS. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-09-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/519 10.55816/mpi.v31i3.519 Majalah Patologi Indonesia; Vol 31. No. 3, September 2022 2527-9106 0215-7284 10.55816/mpi.v31i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/519/378 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/520 2025-12-19T17:06:28Z patologi:ART driver

Histomorphological and Demographic Profile of Breast Carcinoma in Anatomical Pathology Department FKUI/RSCM 2016-2019 Profil Demografik dan Temuan Histomorfologik Karsinoma Payudara di Departemen Patologi Anatomik FKUI/RSCM Periode 2016-2019 Tinjauan Khusus pada Temuan Mitosis Atipik Juwitasari Wongkar, Fresia Hellyanti, Tantri Hayati, Amal Invasive breast carcinoma, histologic type, IBC of NST, atypical mitosis Karsinoma payudara, tipe histologik, karsinoma NST, mitosis atipik BackgroundInvasive breast carcinoma (IBC) is the most commonly diagnosed cancer in woman in the world and Indonesia. IBC is classified into two main categories: IBC of no special type (NST) and special types. This retrospective study aims to provide histomorphological profile of mastectomy cases of IBC in Anatomical Pathology Department, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital (PA FKUI/RSCM) 2016-2019.MethodsThis is a descriptive research with cross-sectional design of mastectomy cases, using secondary data from the archives of PA FKUI/RSCM, starting from 2016- 2019. Collecting clinical data as well as observing several histopathologic parameters including histological type, grade, atypical mitotic features, lymphovascular invasion, and lymph node metastases. Tumour classification was made based on the WHO breast criteria in 2019.ResultsDuring the 2016-2019 period there were 144 cases of IBC. 99.3% of cases occurred in female patients, mostly in the age group from 40 to 59 years and unilateral. The majority had tumour sizes ranging >2-5 cm and were stage II. About a third of cases had received neoadjuvant chemotherapy, most commonly in advanced local stage tumors. Most histologic type (65.3%) were IBC of NST and 52.8% of cases were grade 2. There were 92 (63.4%) cases with atypical mitotic features, most commonly found in stage III. Lymphovascular invasion was found in 38.9% of cases and lymph node metastasis was found in 63.2% of cases.ConclusionIBC is the most common cancer in woman, with the most common histologic type were IBC, grade 2, stage II or more. Most of the cases show atypical mitosis, which is most commonly found in stage III. The findings of lymphovascular invasion did not always coincide with the findings of lymph node metastases. Latar belakangKarsinoma payudara merupakan keganasan tersering pada perempuan di dunia dan di Indonesia. Karsinoma payudara dikelompokkan menjadi dua kategori utama yaitu karsinoma invasif no special type (NST) dan special types. Penelitian retrospektif ini dilakukan untuk mengetahui profil histomorfologik kasus mastektomi karsinoma payudara di Departemen Patologi Anatomik FKUI/RSCM pada tahun 2016-2019.Metode Penelitian deskriptif dengan desain potong lintang kasus mastektomi dari data sekunder arsip Departemen Patologi Anatomik FKUI/RSCM tahun 2016-2019. Dilakukan pengumpulan data klinik serta pengamatan beberapa parameter histopatologik meliputi tipe histologik, grade,gambaran mitosis atipik, invasi limfovaskular, dan metastasis kelenjar getah bening (KGB). Penilaian tipe histologik tumor berdasarkan kriteria WHO breast tahun 2019. HasilSelama periode 2016-2019 terdapat 144 kasus mastektomi karsinoma payudara; 99,3% kasus terjadi pada pasien perempuan, terbanyak pada rentang usia 40-59 tahun dan unilateral. Ukuran tumor tersering berada pada rentang >2-5 cm dan stadium II. Sekitar sepertiga kasus sudah mendapatkan terapi kemoterapi neoadjuvant, tersering pada tumor stadium lokal lanjut. Tipe histologik terbanyak (65,3%) berupa karsinoma invasif NST dan sebanyak 52,8% kasus merupakan tumor grade 2. Didapatkan 92 (63,4%) kasus dengan gambaran mitosis atipik, yang paling sering ditemukan pada stadium III. Invasi limfovaskular ditemukan pada 38,9% kasus dan metastasis KGB ditemukan pada 63,2% kasus.KesimpulanKarsinoma payudara terbanyak pada pasien perempuan dengan histologik tersering berupa karsinoma invasif NST, grade 2, stadium II.Sebagian besar kasus memperlihatkan adanya mitosis atipik, yang paling sering ditemukan pada stadium III. Temuan invasi limfovaskular tidak selalu sejalan dengan temuan metastasis KGB. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2022-09-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/520 10.55816/mpi.v31i3.520 Majalah Patologi Indonesia; Vol 31. No. 3, September 2022 2527-9106 0215-7284 10.55816/mpi.v31i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/520/379 https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/524 2025-02-19T05:27:36Z patologi:ART

oai:ojs.pkp.sfu.ca:article/526 2025-12-19T17:03:52Z patologi:ART driver

Quantitative Histomorphometrical Analysis in Distinguishing Non-Hodgkin Malignant Lymphoma, Large Cell Type with Nasopharyngeal Carcinoma Husain, Okky Aminah Usman, Hermin Yantisetiasti, Anglita Handayani, Astri S Hernowo, Bethy Computational pathology digital pathology Histomorphometry lymphoma malignant nasopharyngeal carcinoma Background Non-Hodgkin’s Malignant Lymphomas (NHMLs) with large cell morphology have similar histopathological appearance with Nasopharyngeal Carcinoma, undifferentiated type therefore ancillary examination such as Immunohistochemistry (IHC) are required. Quantitative histomorphometrical analysis able to identify subtle detail. The aim of this study is to measure the accuracy of quantitative histomorphometrical analysis to distinguishing NHML, large cell type with Nasopharyngeal Carcinoma.   Methods Up to 33 Hematoxylin and Eosin (HE) slides from NHML, large cell type, and 27 cases of Nasopharyngeal Carcinoma were photographed under 400 times magnification with a total of 7131 images. Background, nuclei, and extra-nuclear components between cells are segmented as mathematical objects. Objects’ properties were measured and represented as distribution factors (minimum, quartile 1, median, quartile 3, maximum, range, and interquartile range). Significant factors that can differentiate the study groups are measured with a fisher-exact statistical test. Models to predict NHML, large cell type from nasopharyngeal carcinoma were formulated with different numbers of factors. The HE diagnosis accuracy compared to IHC was measured   Results A sum of 7131 with 3893 from NHMLs, large cell type, and 3238 images of nasopharyngeal carcinoma were collected. After features selection, up to 76 properties factors are selected. Models' accuracy are ranging from 72.67% with a single factor and up to 89.9% with the whole selected factors.   Summary Models’ accuracy are higher (72.6-89.9%) compared to HE primary diagnosis (60%) in distinguishing NHML, large cell againts nasopharyngeal carcinoma. Quantitative histomorphometry can assist pathologist for early screening to distinguishing NHML, large cell type with Nasopharyngeal Carcinoma. Latar Belakang Neoplasma Non-Hodgkin’s Malignant Lymphomas (NHML) sel besar memiliki gambaran histopatologis serupa Karsinoma Nasofaring yang tidak berdiferensiasi sehingga diperlukan pemeriksaan tambahan seperti Immunohistokimia (IHK). Analisis histomorfometri kuantitatif dapat membantu seleksi identifikasi dan mengukur secara digital histopatologi dengan detail. Studi ini bertujuan mengukur akurasi histomorfometri dalam membedakan NHML sel besar dengan Karsinoma Nasofaring. Metode  Sediaan Hematoksilin dan Eosin (HE) dari NHML sel besar sebanyak 33 kasus dan karsinoma nasofaring sebanyak 27 kasus dilakukan pengambilan gambar dibawah perbesaran 400x dengan total 7131 gambar. Dilakukan segmentasi latar belakang, nukleus, dan komponen ekstra nukleus antara sel menjadi himpunan objek matematis. Properti dari objek gambar  (intensitas, saturasi, area, keliling dan lain-lain) diukur dan direpresentasikan dalam faktor distribusi (nilai minimum, kuartil 1, median, kuartil 3, nilai maksimum, rentang dan interquartile range). Signifikansi faktor dalam membedakan kedua kelompok dihitung menggunakan uji statistik fisher-exact. Diformulasikan berbagai model dalam memprediksi NHML sel besar dari Karsinoma Nasofaring dengan berbagai kombinasi faktor. Dilakukan pengukuran akurasi diagnosis HE terhadap hasil IHK. Hasil Didapatkan 7131 gambar dengan 3893 gambar dari NHML sel besar dan 3238 gambar dari karsinoma nasofaring. Setelah diseleksi, didapatkan sebanyak 76 faktor properti. Model menggunakan kombinasi mulai dari 1 faktor sampai 76 faktor dengan akurasi 72.6% hingga 89.9%. Hasil akurasi HE adalah 60%. Kesimpulan Akurasi model lebih tinggi (72.6% - 89.9%) daripada akurasi diagnosis HE (60%) dalam membedakan NHML sel besar dengan karsinoma nasofaring. Histomorfometri kuantitatif dapat menjadi metode skrining awal yang lebih baik dalam membedakan antara NHML sel besar dan karsinoma nasofaring. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2024-01-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/526 10.55816/mpi.v33i1.526 Majalah Patologi Indonesia; Vol. 33 No. 1, Januari 2024 2527-9106 0215-7284 10.55816/mpi.v33i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/526/425 Copyright (c) 2024 Majalah Patologi Indonesia https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/553 2025-12-19T17:00:03Z patologi:ART driver

Thyroid Leiomyosarcoma: A Rare Case Report Juanita Maulana, Aries Yamin, Amalia prihatni, Imeldi Sungowati, Ni Ketut Miskad, Upik Anderiani Keywords: Thyroid, Leiomyosarcoma, Immunohistochemistry Kata kunci: Tiroid, Leiomyosarcoma, Imunohistokimia Background Primary thyroid leiomyosarcoma is a very rare tumor. The incidence is less than 0.01% of all primary thyroid tumors. Thyroid leiomyosarcoma are tumours showing smooth muscle differentiation, originating from thyroid gland vascular smooth muscle. Case Description Female, 47 years old, with lump on the left neck since 1 year ago, getting bigger in the last 3 months, no shortness of breath, no history of previous surgery. Physical examination revealed a mass was 13x6 cm in size, solid, firm.  Cytological features from fine needle aspiration biopsy (FNAB) showed cellular spindle cells with atypical, pleomorphic, hyperchromatic nuclei and  several normal thyroid follicles among them, sugesting  malignant thyroid tumor (Bethesda VI). Total thyroidectomy was performed, followed by histopathological examination. Microscopically showed proliferation of atypical, pleomorphic, hyperchromatic spindle cells with eosinophilic cytoplasm. The spindle cells arranged in fascicular and interlacing pattern. Several follicular thyroid are entrapped within this atypical spindle cells. Based on these finding, this case was diagnosed as anaplastic thyroid carcinoma. To confirm the diagnosis, immunohistochemical examination with cytokeratin, desmin and Ki-67 were performed and the result showed negative for cytokeratin, positive for desmin and Ki-67 was highly positive. Based on these results, a diagnosis of thyroid leiomyosarcoma was made. Discussion and Conclusion Differential diagnosis with other primary mesenchymal thyroid  tumors should be considered in the cases with atypical spindle cells microscopic appearance. The diagnosis of thyroid leiomyosarcoma based on cytology and histopathology is difficult to establish, therefore immunohistochemical staining is required to establish the accurate final diagnosis. Latar belakang: Leiomyosarcoma tiroid primer merupakan tumor yang sangat jarang. Insidennya kurang dari 0,01% dari semua tumor tiroid primer. Leiomyosarcoma tiroid adalah tumor yang menunjukkan diferensiasi otot polos, yang berasal dari otot polos pembuluh darah kelenjar tiroid. Keterangan Kasus: Wanita, 47 tahun, dengan benjolan di leher kiri sejak 1 tahun yang lalu, semakin membesar dalam 3 bulan terakhir, tidak ada sesak nafas, tidak ada riwayat operasi sebelumnya. Pemeriksaan fisik didapatkan massa berukuran 13x6 cm, padat, keras. Gambar sitologis dari biopsi aspirasi jarum halus (FNAB) menunjukkan sel gelendong seluler dengan inti atipikal, pleomorfik, hiperkromatik dan beberapa folikel tiroid normal di antaranya, menunjukkan tumor tiroid ganas (Bethesda VI). Dilakukan tiroidektomi total, dilanjutkan dengan pemeriksaan histopatologi. Secara mikroskopis menunjukkan proliferasi sel spindle atipikal, pleomorfik, hiperkromatik dengan sitoplasma eosinofilik. Sel-sel gelendong disusun dalam pola fasikuler dan jalinan. Beberapa tiroid folikuler terperangkap di dalam sel gelendong atipikal ini. Berdasarkan temuan tersebut, kasus ini didiagnosis sebagai karsinoma tiroid anaplastik. Untuk memastikan diagnosis dilakukan pemeriksaan imunohistokimia dengan sitokeratin, desmin dan Ki-67 dan hasilnya menunjukkan sitokeratin negatif, desmin positif dan Ki-67 sangat positif. Berdasarkan hasil tersebut, diagnosis leiomyosarcoma tiroid dibuat. Diskusi dan Kesimpulan: Diagnosis banding dengan tumor tiroid mesenkimal primer lainnya harus dipertimbangkan pada kasus dengan mikroskop sel spindel atipikal. Diagnosis leiomyosarcoma tiroid berdasarkan sitologi dan histopatologi sulit ditegakkan, oleh karena itu diperlukan pewarnaan imunohistokimia untuk membuat diagnosis akhir yang akurat.   Kata kunci: Tiroid, Leiomyosarcoma, Imunohistokimia Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2025-01-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/553 10.55816/mpi.v33i3.553 Majalah Patologi Indonesia; Vol. 34 No. 1, Januari 2025 2527-9106 0215-7284 10.55816/mpi.v34i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/553/453 Copyright (c) 2025 Juanita, Aries Maulana, Amalia Yamin, Imeldi prihatni, Ni Ketut Sungowati, Upik Anderiani Miskad https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/560 2025-12-19T17:04:13Z patologi:ART driver

Lymph Nodes Metastasis is Associated with Higher Body Mass Index in Indonesian Breast Cancer Patients Yournita, Debbie Rustamadji, Primariadewi karsinoma payudara invasif, indeks massa tubuh, overweight, obesitas, profil klinikopatologik, metastasis kelenjar getah bening invasive breast carcinoma, body mass index, overweight, obesity, clinical pathological profile, lymph node metastasis Background Breast cancer is the most prevalent malignancy among women and is the second largest contributor to cancer-related deaths globally. Women with a high body mass index (BMI) are at a greater risk of developing breast cancer and an increased risk of lymph node metastasis. This retrospective study aimed to determine the relationship between BMI and the clinical pathological profile of invasive breast carcinoma (IBC) patients. Method This study was conducted from 2019 to 2022. Data were collected through the archives of the Department of Anatomic Pathology FKUI/RSCM (Faculty of Medicine, University of Indonesia/Cipto Mangunkusumo Hospital). Clinical pathological data were collected. The histological type, grade, lymphovascular invasion, and lymph node metastasis of the tumors were re-evaluated. The chi-square hypothesis test was used to determine the relationship between BMI and clinical pathological profile. The results were considered statistically significant with a p <0.05. Result A total of 151 cases of IBC with lymph node dissection from 2019 to 2022 were included in this study, all of which were female, with the majority being >50 years old (55%). The most common tumor size and histological type were T2 (39,7%) and invasive ductal carcinoma NST (74,8%), 49% of cases were grade 3 tumors. Most IBC cases had an obese BMI (49%), 63.1% with lymph node metastasis had an overweight-obese BMI. Statistical analysis was performed between BMI and lymph node metastasis status, and p-value=0.025 was obtained. The relationship between tumor size and lymphovascular invasion with lymph node metastasis was also found to be statistically significant, as indicated by a p-value of 0.000. Conclusion Cases with lymph node metastasis had the highest rate of overweight-obesity, and a significant relationship was found between BMI and lymph node metastasis status. A significant relationship was also found between tumor size and lymphovascular invasion with lymph node metastasis. Latar belakang Kanker payudara merupakan kanker paling sering pada wanita dan menjadi penyebab kematian kedua tersering dari seluruh kanker di dunia. Wanita dengan indeks massa tubuh (IMT) yang berlebih memiliki risiko lebih tinggi terjadinya kanker payudara dan meningkatkan risiko metastasis KGB. Penelitian retrospektif ini dilakukan untuk mengetahui hubungan indeks massa tubuh (IMT) dengan profil klinikopatologik pasien karsinoma payudara invasif (KPI). Metode Penelitian ini merupakan penelitian retrospektif dengan pengumpulan data dilakukan melalui arsip Departemen PA FKUI/RSCM periode Januari 2019 hingga November 2022. Dilakukan penelusuran data klinikopatologik dan penilaian ulang tipe histologik tumor, grade, invasi limfovaskular dan metastasis KGB. Penilaian statistik menggunakan uji hipotesis chi square untuk mengetahui hubungan IMT dengan status metastasis KGB. Hasil dinyatakan bermakna secara statistik bila nilai p<0.05. Hasil Selama periode Januari 2019 hingga November 2022 terdapat 151 kasus mastektomi KPI disertai pengangkatan KGB, seluruhnya berjenis kelamin perempuan, terbanyak berusia >50 tahun (55%). Ukuran tumor tersering yaitu T2 (39,7%). Tipe histologik terbanyak berupa karsinoma invasif NST (74,8%) dan 49% kasus merupakan tumor grade 3. Mayoritas kasus KPI memiliki IMT obesitas (49%). Sebanyak 63,1% kasus KPI dengan metastasis KGB memiliki IMT overweight-obesitas. Dilakukan uji statistik IMT dan status metastasis KGB, didapatkan nilai p=0,025. Dilakukan pula uji statistik terhadap ukuran tumor dan invasi limfovaskular dengan metastasis KGB, didapatkan nilai p=0,000. Kesimpulan Kasus dengan metastasis KGB terbanyak memiliki IMT overweight-obesitas. Didapatkan hubungan yang bermakna antara indeks massa tubuh dengan status metastasis KGB. Didapatkan pula hubungan yang bermakna antara ukuran tumor dan invasi limfovaskular dengan metastasis KGB. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2023-09-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/560 10.55816/mpi.v32i3.560 Majalah Patologi Indonesia; Vol. 32 No. 3, September 2023 2527-9106 0215-7284 10.55816/mpi.v32i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/560/409 Copyright (c) 2023 Majalah Patologi Indonesia https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/561 2025-12-19T17:05:41Z patologi:ART driver

Relationship between Clinicohistomorphology Profile of Atypical Prostate Gland and Diagnosis Benign Lesion or Prostate Adenocarcinoma Litta Septina MZ Yayi Dwina B Atypical prostate gland atypical small acinar proliferation prostate adenocarcinoma BackgroundAtypical prostate gland especially atypical small acinar proliferation (ASAP) is a histopathological diagnosis that requires a follow-up biopsy 3-6 months after the first biopsy, because 17-60% of cases potentially to be malignant. The varied clinical and histomorphological characteristics of the atypical prostate gland make it difficult to confirm the final diagnosis as a benign lesion or prostate adenocarcinoma. This study aims to describe clinicohistomorphological cases of atypical prostate gland at Anatomical Pathology Department FKUI/RSCM and to identify histomorphological features of the atypical prostate gland as benign lesions or prostate adenocarcinoma on immunohistochemistry diagnosis.MethodsA histopathological investigation of prostate gland cases with atypical nuclei was carried out in 2011-2021 from archives of Anatomical Pathology Department FKUI/RSCM. Clinical and histomorphological characteristics were assessed and categorized into benign lesions or prostate adenocarcinoma based on immunohistochemistry appearance.ResultsThere were 109 cases of atypical prostate gland, 49 of which met the inclusion and exclusion criteria and could be analyzed. Corpora amylacea was found in 11 cases (84.6%) in the benign lesion group, statistically significant (p-value 0.005). Intraluminal crystalloids were found in 4 cases (100%) in the prostate adenocarcinoma group with p-value 0.050, close to significant. Other clinicohistomorphological characteristics did not show a significant relationship both in benign lesions and prostate adenocarcinoma groups (p-value 0.05).Conclusion On histopathological examination of the atypical prostate gland that is difficult to re-biopsy, the discovery of corpora amylacea may lead to the diagnosis of a benign lesion, while the discovery of intraluminal crystalloids may lead to the diagnosis of prostate adenocarcinoma. The diagnosis must be supported by immunohistochemistry characteristics. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2023-02-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/561 10.55816/mpi.v32i1.561 Majalah Patologi Indonesia; Vol. 32 No. 1, Januari 2023 2527-9106 0215-7284 10.55816/mpi.v32i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/561/390 Copyright (c) 2023 Majalah Patologi Indonesia https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/562 2025-12-19T17:05:41Z patologi:ART driver

Clinical and Histopathological Features of Non-Hodgkin Lymphoma in Gastrointestinal System at RSUP Dr. Hasan Sadikin Bandung 2018-2020 Gambaran Klinis dan Histopatologis Limfoma Non Hodgkin Sistem Pencernaan di RSUP Dr. Hasan Sadikin Bandung Tahun 2018-2020 Maharani Tasyandita, Raina Suryawathy Hernowo, Bethy Yulianti, Herry limfoma non hodgkin limfoma sistem pencernaan gambaran klinis histopatologi Non Hodgkin lymphoma gastrointestinal lymphoma clinical features histopathology BackgroundNon-Hodgkin's lymphoma (NHL) is an abnormal growth of lymphocytes that manifests as tumor or enlargement of lymph nodes (lymphadenopathy). New NHL cases in Indonesia in 2020 reached 16.125 cases (4.1%) with 9.024 deaths (3.6%). The WHO classification categorizes NHL into two, B cells and T/NK cells.2 NHL is also divided into low-grade and high-grade based on its progression. The gastrointestinal tract is the most frequently involved extranodal site in NHL. The clinical manifestations of NHL can be non-specific and vary depending on anatomic location. This study aims to determine the clinical and histopathological features in patients with gastrointestinal NHL.MethodsThis research is a descriptive study using medical record data of inpatients at RSUP Dr. Hasan Sadikin Bandung period 2018-2020 diagnosed with gastrointestinal NHL.ResultsPatient characteristics were dominated by patients aged 40 years and male (65.2%), with the most clinical manifestations being enlarged lymph nodes. NHL most often attacks the liver (26.1%) and most patients present at stage IV (34.8%). The most histopathological features were B cells (52.5%) and high-grade (43.5%).ConclusionThe risk of gastrointestinal LNH increases with age and the majority is in men. Clinical manifestations vary and can show local or constitutional symptoms. Most patients are found to be in the late stages and among all gastrointestinal system, most NHL attacks the liver. Many patients were not subjected to further immunohistochemical examination, but the most common histopathological features were B cells and high grade NHL. PendahuluanLimfoma non Hodgkin (LNH) adalah pertumbuhan abnormal limfosit yang bermanifestasi menjadi tumor atau pembesaran kelenjar getah bening (KGB). Jumlah kasus baru LNH di Indonesia pada tahun 2020 mencapai 16.125 kasus (4,1%) dengan 9.024 kematian (3,6%). Klasifikasi dari WHO mengkategorikan LNH menjadi dua, yaitu sel B serta sel T/NK. LNH juga dibedakan menjadi low-grade dan high-grade berdasarkan progresivitasnya.1 Sistem pencernaan merupakan lokasi ekstranodal yang paling sering terlibat dalam LNH. Manifestasi klinis LNH dapat bersifat non spesifik dan bervariasi tergantung lokasi anatominya. Penelitian ini bertujuan untuk mengetahui gambaran klinis dan histopatologis pada pasien LNH sistem pencernaan.MetodePenelitian ini merupakan penelitian deskriptif menggunakan data rekam medis pasien rawat inap RSUP Dr. Hasan Sadikin Bandung periode 2018-2020 yang terdiagnosis LNH sistem pencernaan.HasilKarakteristik pasien didominasi oleh pasien berusia ≥40 tahun dan berjenis kelamin laki-laki (65,2%), dengan manifestasi klinis terbanyak adalah pembesaran KGB. LNH paling sering menyerang hepar (26,1%) dan kebanyakan pasien datang pada stadium IV (34,8%). Gambaran histopatologi yang paling banyak adalah sel B (52,5%) serta high-grade (43,5%).KesimpulanRisiko LNH sistem pencernaan meningkat seiring dengan pertambahan usia dan mayoritas adalah pada laki-laki. Manifestasi klinis pada LNH sistem pencernaan bervariasi dan dapat menunjukkan gejala lokal maupun konstitutional. Pasien paling banyak ditemukan berada pada stadium akhir dan diantara seluruh sistem pencernaan, kebanyakan LNH menyerang hepar. Sebagian besar pasien tanpa pemeriksaan imunohistokimia lebih lanjut, namun gambaran histopatologi yang umum ditemukan adalah LNH yang berasal dari sel B serta dengan high-grade. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2023-02-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/562 10.55816/mpi.v32i1.562 Majalah Patologi Indonesia; Vol. 32 No. 1, Januari 2023 2527-9106 0215-7284 10.55816/mpi.v32i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/562/391 Copyright (c) 2023 Majalah Patologi Indonesia https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/564 2025-12-19T17:05:41Z patologi:ART driver

Correlation of Microvessel Density with Clinicothological Features of Hodgkin Lymphoma Bima Zulfikar, M. Murti, Krisna Andriyani Liberty, Iche Andriyani Liberty, Iche Dewi, Citra Sari Puspita Rasyid, Riana hodgkin lymphoma microvessel density CD34 clinicopathological characteristics BackgroundAngiogenesis is the process of new blood vessel growth that has an important role in tumor development. Microvessel density (MVD) assessment is one of the methods to assess angiogenesis. A high MVD is associated with a poor prognosis in various types of cancer including Hodgkin lymphoma (HL). Although, HL incidence is lower in developing countries, the death rate is higher in these regions. Moreover, this type of lymphoma is higher among young and reproductive people. The aim of this study was to determine the association between microvessel density and HL clinicopathological characteristics.MethodsThis was an observational analytic study with a cross-sectional method, conducted at the Anatomical Pathology Department/Dr. Mohammad Hoesin Palembang. The samples were paraffin blocks diagnosed as HL for period 2016-2021 and had complete clinicopathological data according to the criteria of this study. The slides were immunostained with antibody anti-CD34. One microvessel was determined by observing the endothelial morphology and CD34 expression in endothelial cytoplasm and/or cell membrane, then was evaluated and calculated by Image J. Data was statistically analyzed by STATA version 15.ResultsMajority HLs in this study were discovered in the age group of <45 years (71.00%), male (61.30%), nodal location (80.60%) and lymphocyte rich classical HL (LRCHL) subtype (51.60%). Although there was no significant association between MVD and the clinicopathological characteristics of HL, high MVD tend to occur in the age <45 years, male, nodal location, and LRCHL subtype.ConclusionThere was no significant association between microvessel density and HL clinicopathological characteristics. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2023-02-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/564 10.55816/mpi.v32i1.564 Majalah Patologi Indonesia; Vol. 32 No. 1, Januari 2023 2527-9106 0215-7284 10.55816/mpi.v32i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/564/392 Copyright (c) 2023 Majalah Patologi Indonesia https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/565 2025-12-19T17:05:41Z patologi:ART driver

Clinicopathologic and Immunohistochemistry Profile of Primary Mediastinal Large B-cell Lymphoma and Hodgkin Lymphoma Nodular Sclerosis in Mediastinum Klinikopatologik dan Profil Imunohistokimia Primary Mediastinal Large B-cell Lymphoma dan Limfoma Hodgkin Nodular Sclerosis di Regio Mediastinum Dameria, Flora Francisca Ham, Maria Stephanie Harahap, Agnes Primary mediastinal large B-cell lymphoma limfoma Hodgkin nodular sklerosis mediastinum Primary mediastinal large B-cell lymphoma Hodgkin lymphoma nodular sclerosis mediastinal Background Primary mediastinal large B-cell lymphoma (PMBCL) and Hodgkin nodular sclerosis (LHNS) lymphoma was the most common lymphomas involved the mediastinum. These two lymphomas had similar histopathological features. The purpose of this study was to analyzed clinical data, histopathological characteristics, and immunohistochemistry (IHC) profiles on PMBCL and LHNS. Methods This is a retrospective study. Data was collected from archive of Anatomical Pathology Department, Faculty of Medicine, Universitas Indonesia/Cipto Mangunkusumo Hospital in 2014-2020. The data collected included clinical data, hematoxylin-eosin (HE) and IHC slides and were reassessed by the researcher and supervisor. The histopathological characteristics data was analyzed statistically using statistical package for the social sciences version 25.0 with Chi-Square test. Results PMBCL and LHNS have similar histopathological features, included the characteristics of tumor cells and infiltration of many fibrosis and inflammatory cells, but difference in architecture, tumor cell types, clear cytoplasmic tumor cells, inflammatory cell and fibrosis types, with p value <0.001. The characterized differences of PMBCL showed diffuse architecture, some cells with RS-like cell structure, clear/pale cytoplasm, lymphocyte cell infiltration, and compartmental fibrosis. Whilst, LHNS showed nodular architecture, Reed Sternberg cells, mixed inflammatory cell infiltration, and band-like fibrosis. PMBCL showed CD20, PAX5, MUM1 strongly positive, CD30 weakly positive, and CD15 negative, while LHNS showed negative CD20, CD30 and MUM1 strongly positive, PAX5 weakly positive, and CD15 positive.   Latar belakang Primary mediastinal large B-cell lymphoma (PMBCL) dan limfoma Hodgkin nodular sclerosis (LHNS) merupakan limfoma tersering yang melibatkan mediastinum. Kedua limfoma ini memiliki kemiripan gambaran histopatologi. Tujuan penelitian ini untuk menganalisis karakteristik histopatologi dan profil imunohistokimia (IHK) pada PMBCL dan LHNS. Metode Penelitian ini merupakan penelitian retrospektif analitik dengan pengumpulan data dari arsip Departemen Patologi Anatomik Fakultas Kedokteran Universitas Indonesia/RSUPN Dr. Cipto Mangunkusumo periode 2014-2020. Data yang dikumpulkan berupa data klinis, slaid hematoksilin-eosin (HE) dan IHK, yang dinilai ulang oleh peneliti dan pembimbing. Data karakteristik histopatologi dianalisis secara statistik menggunakan statistical package for the social sciencesversi 25.0 dengan uji Chi-Square. Hasil PMBCL dan LHNS memiliki gambaran histopatologi serupa, berupa sel tumor dengan sebukan sel radang dan fibrosis; namun berbeda dalam arsitektur, jenis sel tumor, sel tumor bersitoplasma jernih, jenis sel radang dan fibrosis, dengan nilai p<0,001. Perbedaan karakteristik adalah PMBCL menunjukkan arsitektur difus, sel dengan struktur sel RS-like, sitoplasma jernih/pucat, sebukan sel limfosit, dan fibrosis kompartemen. Sedangkan, LHNS menunjukkan arsitektur nodular, sel Reed Sternberg, sebukan sel radang campuran, dan fibrosis band-like. PMBCL memberikan hasil CD20, PAX5, MUM1 positif kuat, CD30 positif lemah, dan CD15 negatif; sedangkan LHNS memberikan hasil CD20 umumnya negatif, CD30 dan MUM1 positif kuat, PAX5 positif lemah, dan CD15 positif. Kesimpulan Ekspresi gen CD20, CD30, CD15, PAX5 dapat digunakan untuk membedakan PMBCL dan LHNS. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2023-02-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/565 10.55816/mpi.v32i1.565 Majalah Patologi Indonesia; Vol. 32 No. 1, Januari 2023 2527-9106 0215-7284 10.55816/mpi.v32i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/565/393 Copyright (c) 2023 Majalah Patologi Indonesia https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/566 2025-12-19T17:05:41Z patologi:ART driver

Extranodal NK/T-cell Lymphoma, Nasal Type (Angiocentric T-cell Lymphoma): A Case Review of Histopathological Diagnosis Extranodal NK/T-cell Lymphoma, Nasal Type (Angiocentric T-cell Lymphoma): Laporan Kasus dan Kajian Diagnosis Histopatologik Prasetyo, Awal Istiadi, Hermawan Puspasari, Dik Extranodal NK/T-cell lymphoma, nasal type diagnosis histopatologi panel imunohistokimia Pansitokeratin (AE1/3) LCA CD3 CD56 Extranodal NK/T-cell lymphoma nasal type histopathological diagnosis immunohistochemical panel of Pansitokeratin (AE1/3) LCA CD3 CD56 Extranodal NK/T-cell lymphoma, nasal type (ENKL) is a lymphoid neoplasm, which in the past has been grouped as a type of granulomatous diseases, is an aggressive non-Hodgkin’s lymphoma. The aggressiveness of ENKL is characterized clinically by nonrelenting destruction of the midline structures of the palate and nasal fossa. The histopathological diagnosis of this disease is difficult to enforced despite the malignant clinical course recognizably because of extensive tissue necrosis and multiple biopsies are often required. ENKL has an ominous prognosis, as the average survival rate is between 6 and 25 months. This case report describes the difficulties in making histopathological diagnosis of a 52-year-old male patient who presented with nasal obstruction and foul smelling, destructive and ulseratif lesion over for 3 months duration, and failed to treat with antibiotics and anti-inflammatories drugs. The diiferential diagnosis of the multiple biopsies was nasal cavity granuloma, NK/T cell lymphoma and Wegener granulomatosis. After immunohistochemical panel analysis of Pansitokeratin (AE1/3), LCA, CD3 and CD56, the patient be confirmed had an ENKL, nasal type which in the past also known as angiocentric T-cell lymphoma. Extranodal NK/T-cell lymphoma, nasal type (ENKL) adalah neoplasma limfoid yang dikelompokkan dalam ragam penyakit granulomatosa, dan merupakan limfoma non-Hodgkin yang bersifat agresif. Agresivitas ENKL ditandai dengan kemampuan destruksi yang hebat dan persisten (nonrelenting) pada stuktur linea mediana palatum dan fossa nasalis. Diagnosis histopatologi kelainan ini sulit ditegakkan walaupun secara klinis gambaran keganasannya nampak nyata, karena banyaknya jaringan nekrotik yang ekstensif dan seringkali memerlukan tindakan biopsi multipel dengan prognosis perforasi dan destruksi wajah yang menakutkan, dengan ketahanan hidup berkisar antara dalam 6 sampai 25 bulan. Laporan kasus ini mendeskripsikan kesulitan diagnosis histopatologi pada laki-laki berusia 52 tahun yang mengeluh hidung tersumbat berbau busuk, dengan lesi destruktif di septum nasi dan ulseratif di palatum selama 3 bulan, serta gagal diterapi dengan antibiotik dan anti-inflamasi. Diagnosis banding berdasarkan hasil pemeriksaan biopsi multipel yaitu sebagai granuloma cavum nasi, limfoma NK/T cell dan Wegener granuloma. Setelah dilakukan pemeriksaan panel imunohistokimia dengan Pansitokeratin (AE1/3), LCA, CD3 dan CD56 maka dikonfirmasi diagnosis ENKL, nasal type, atau juga dikenal sebagai angiocentric T-cell lymphoma. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2023-02-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/566 10.55816/mpi.v32i1.566 Majalah Patologi Indonesia; Vol. 32 No. 1, Januari 2023 2527-9106 0215-7284 10.55816/mpi.v32i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/566/394 Copyright (c) 2023 Majalah Patologi Indonesia https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/567 2025-12-19T17:05:41Z patologi:ART driver

The Role of Bone Marrow Biopsy Morphology and Clinical Characteristics in Facing the Challenges of Diagnosing Primary Myelofibrosis, Polycythemia Vera, and Essential Thrombocythemia at Cipto Mangunkusumo Hospital Rifani, Liyona Stephanie, Agnes Sukrisman, Lugyanti Mira Lubis, Anna Francisca Ham, Maria Myeloproliferative neoplasm polycythemia vera primary myelofibrosis essential thrombocythemia BackgroundMyeloproliferative neoplasm (MPN) is a neoplasm characterized by the proliferation of one or more myeloid cells and their derivatives. The limitations of molecular examination in Indonesia make the diagnosis of MPN based on clinical and histopathological examination very crucial. The aim evaluate the clinicopathological profile of primary myelofibrosis (PMF), polycythemia vera (PV), and essential thrombocythemia (ET) and identify the typical morphological characteristics of bone marrow biopsy.MethodsA retrospective study of cases diagnosed with MPN was conducted at the Department of Anatomic Pathology FMUI/RSCM in 2015-2019. Clinical data and evaluation of bone marrow morphology consisting of cellularity, erythroid myeloid ratio, cluster and megakaryocyte morphology, blast cells, fibrosis, osteosclerosis, and sinus dilatation were analyzed.ResultsA total of 172 cases were diagnosed as MPN BCR-ABL1-negative (PMF: 74; ET: 56; PV: 42). On routine blood examination, there was an increase in hemoglobin (Hb) and hematocrit (Ht) in PV, a decrease in Hb in PMF and an increase in platelets in ET (p<0.001). Splenomegaly is mostly found in PMF. Myeloid erythroid ratio was decreased in PV, normal in ET and increased in PMF (p<0.001). Megakaryocytes were arranged in loose clusters in 88.1% of PV cases and 96.4% of ET, dense clusters were found in 91.9% of PMF cases. Staghorn-like megakaryocytes were found in all ET cases and bulbous/cloud-like megakaryocytes were found in 97.3% of PMF cases. Most cases of PV (90.5%) and all cases of ET showed pre-fibrotic bone marrow (grade 0-1), while 77% of PMF cases showed fibrosis grade 2-3.ConclusionIn diagnosing PV, ET, and PMF, correlation between clinical data, laboratory, and bone marrow histomorphological evaluations, especially cellularity, myeloid:erythroid ratio, cluster and megakaryocyte morphology, degree of fibrosis, osteosclerosis, and sinus dilatation is required. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2023-02-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/567 10.55816/mpi.v32i1.567 Majalah Patologi Indonesia; Vol. 32 No. 1, Januari 2023 2527-9106 0215-7284 10.55816/mpi.v32i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/567/395 Copyright (c) 2023 Majalah Patologi Indonesia https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/568 2025-12-19T17:05:41Z patologi:ART driver

The Association Between Expression of Transforming Growth Factor-β1, Fibroblast Activated Protein, Pattern of Tumor-Associated Collagen Signature and Degree of Tumor-Infiltrating Lymphocytes to Grading International Society Of Urological Pathology in Clea Hubungan Ekspresi Transforming Growth Factor-β1, Fibroblast Activated Protein, pola Tumor-Associated Collagen Signature dan derajat Tumor-Infiltrating Lymphocytes terhadap Grading International Society of Urological Pathology pada Clear Cell Renal Cell Ca Kharismawaty, Dina Delyuzar, Delyuzar Betty, Betty S Lukito, Joko Chrestella, Jessy TGF-β1 FAP, TACS TILs ISUP grading ccRCC TGF-β1 FAP TACS TILs ISUP grading ccRCC BackgroundClear cell renal cell carcinoma (ccRCC) is a very aggressive cancer and is often diagnosed at an advanced stage. The stromal component around the tumor is thought to have contributed greatly to these findings.MethodsAnalytical study with cross sectional design on 23 histological specimens diagnosed by ccRCC based on data from medical records/pathology archives in 2012-2021. Each specimen was stained with hematoxylin and eosin, TGF-β and FAP immunohistochemistry and Masson's trichrome. Fisher's exact test (p<0.05) was used to assess the association between the expressions of all variables with the ISUP grading.ResultsMost cases were high grade ccRCC (87%) samples, of which 73.9% samples expressed strong TGF-β, 82.7% samples expressed positive intratumoral FAP, 78.3% samples expressed positive stromal FAP, 47.8% showed a TACS-3-pattern, 56.5% of samples with positive stromal TILs and 47.8% of samples with negative intratumoral TILs. There was no significant association between all of these variables and the ISUP grading of the ccRCC (p<0.05).ConclusionExpression of TGF-β1, intratumoral and stromal FAP, TACS pattern and the degree of intratumoral and stromal TILs were not significantly associated with ISUP grading in ccRCC. Latar belakangClear cell renal cell carcinoma (ccRCC) merupakan kanker yang sangat agresif dan sering didiagnosis pada stadium lanjut. Komponen stroma disekitar tumor diduga memberikan kontribusi yang besar terhadap penemuan tersebut.MetodePenelitian analitik dengan desain cross sectional terhadap 23 spesimen histologi yang didiagnosis ccRCC berdasarkan data dari rekam medis/arsip patologi pada tahun 2012-2021. Masing–masing spesimen telah dipulas dengan pewarnaan hematoksilin and eosin, immunohistokimia TGF-β dan FAP serta trichrome Masson. Uji Fisher’s exact test (p<0,05) digunakan untuk menilai hubungan ekspresi seluruh variabel dengan grading ISUPHasilKasus terbanyak merupakan high grade ccRCC (87%) sampel, diantaranya 73,9% sampel terekspresi TGF-β kuat, 82,7% sampel terekspresi FAP intratumoral positif, 78,3% sampel terekspresi FAP stroma positif, 47,8% sampel menunjukkan pola TACS-3, 56,5% sampel dengan derajat TILs stroma positif dan 47,8% sampel dengan derajat TILs intratumoral negatif. Tidak terdapat hubungan yang bermakna antara seluruh variabel tersebut dengan grading ISUP pada ccRCC (p<0,05).KesimpulanEkspresi TGF-β1, FAP intratumoral dan stroma, pola TACS dan derajat TILs intratumoral dan stroma tidak berhubungan secara signifikan dengan grading ISUP pada penderita ccRCC. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2023-02-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/568 10.55816/mpi.v32i1.568 Majalah Patologi Indonesia; Vol. 32 No. 1, Januari 2023 2527-9106 0215-7284 10.55816/mpi.v32i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/568/396 Copyright (c) 2023 Majalah Patologi Indonesia https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/569 2025-12-19T17:05:41Z patologi:ART driver

Clinicopathological Profile of Lymph Node Metastases in Ovarian Carcinoma at Cipto Mangunkusumo Hospital 2016-2018 Profil Klinikopatologik Metastasis Kelenjar Getah Bening pada Karsinoma Ovarium di Departemen Patologi Anatomik FKUI/RSCM 2016-2018 Hayati, Amal Tjahjadi, Hartono Karsinoma ovarium makrometastasis mikrometastasis ovarian carcinoma macrometastasis micrometastasis Background Ovarian cancer is one of the most common malignancies in women. In Indonesia, this incidence is the third highest after breast and cervical cancer. The prognosis for ovarian carcinoma is generally poor. Ovarian cancer patients have low survival rate since most of them diagnosed at advanced stage. The tumor spread to retroperitoneum lymph nodes is important to evaluate considering that metastasis to lymph nodes will upstage the tumor to stage III. Tumor histological type, grade and CA 125 serum level considered as risk factors for lymph node metastases. Methods This study was a cross-sectional descriptive study to determine the profile of lymph node metastases in ovarian carcinoma at the Anatomical Pathology Department FKUI-RSCM for 3 years. The assessment criteria included demographic, clinical, and histopathological features of tumor mass and retroperitoneum lymph nodes. Results Analysis of demographic data in 54 cases of ovarian carcinoma found 47 patients (87%) aged ≥40 years. Unilateral tumors found in 42 cases (77.8%) and bilateral tumors found in 12 cases (22.2%). Patients generally diagnosed at stage III as many as 17 cases (31.4%). Elevated levels of tumor marker CA 125 (≥35 u/ml) found in 41 cases (76%). A total of 19 cases of ovarian carcinoma had spread to lymph nodes, with 8 cases (42%) of which were serous carcinomas. Lymph node metastases found in 16 cases (80%) of high-grade ovarian carcinoma and 12 cases (60%) of advance-staged tumors. Macrometastatic tumor deposit patterns found in 180 lymph nodes and micrometastases found in 10 lymph nodes. Two subjects diagnosed for lymph node metastases only from micrometastasis deposit in lymph nodes. Conclusion Lymph node metastases in ovarian carcinoma are more frequently found in serous carcinoma, high grade carcinoma, and cases with elevated CA125 serum level. Lymph nodes evaluation should be conducted carefully to detect micrometastasis deposit. Latar belakangKanker ovarium merupakan salah satu keganasan tersering pada wanita. Di Indonesia, angka insidensi kanker ovarium menjadi yang terbanyak ketiga pada wanita, setelah kanker payudara dan serviks. Prognosis karsinoma ovarium secara umum buruk dan memiliki tingkat kesintasan rendah karena sebagian besar pasien terdiagnosis pada stadium lanjut. Penyebaran tumor ke kelenjar getah bening retroperitoneum penting untuk dievaluasi mengingat metastasis pada kelenjar getah bening akan langsung menaikkan stadium tumor menjadi stadium III. Perbedaan jenis histopatologik tumor, derajat tumor, dan kadar CA 125 serum diduga memengaruhi metastasis pada kelenjar getah bening.MetodePenelitian ini adalah penelitian deskriptif potong lintang untuk mengetahui profil metastasis kelenjar getah bening pada karsinoma ovarium di Departemen Patologi Anatomik FKUI-RSCM selama 3 tahun. Kriteria penilaian meliputi data demografik, klinis, serta gambaran histopatologik massa tumor dan kelenjar getah bening retroperitoneum.HasilPenelaahan data demografik pada 54 kasus karsinoma ovarium didapatkan 47 pasien (87%) berumur ≥40 tahun. Tumor unilateral ditemukan pada 42 kasus (77,8%) dan tumor bilateral ditemukan pada 12 kasus (22,2%). Pasien umumnya terdiagnosis pada stadium III yaitu sebanyak 17 kasus (31,4%). Peningkatan kadar penanda tumor CA 125 (≥35 u/ml) ditemukan pada 41 kasus (76%). Sebanyak 19 kasus karsinoma ovarium mengalami penyebaran ke kelenjar getah bening, dengan 8 kasus (42%) di antaranya berupa karsinoma serosum. Metastasis kelenjar getah bening ditemukan pada 16 kasus (80%) karsinoma ovarium high grade dan 12 kasus (60%) tumor stadium lanjut. Pola deposit tumor makrometastasis ditemukan pada 180 kelenjar getah bening dan mikrometastasis ditemukan pada 10 kelenjar getah bening. Sebanyak 2 sampel terdiagnosis mengalami metastasis kelenjar getah bening hanya berdasarkan keberadaan deposit tumor mikrometastasis.KesimpulanMetastasis kelenjar getah bening pada karsinoma ovarium lebih sering ditemukan pada karsinoma jenis serosum, karsinoma high grade, dan kasus dengan peningkatan kadar CA 125 serum. Evaluasi kelenjar getah bening penting untuk dilakukan seksama guna mendeteksi keberadaan mikrometastasis. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2023-02-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/569 10.55816/mpi.v32i1.569 Majalah Patologi Indonesia; Vol. 32 No. 1, Januari 2023 2527-9106 0215-7284 10.55816/mpi.v32i1 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/569/397 Copyright (c) 2023 Majalah Patologi Indonesia https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/579 2025-12-09T03:41:56Z patologi:ART

oai:ojs.pkp.sfu.ca:article/580 2025-12-19T17:03:15Z patologi:ART driver

Relationship of Stromal Tumor Infiltrating Lymphocytes (STILs) with Grading Histopathology in Penis Squamous Cell Carcinoma Sihotang, Marisi Cintya Debby Intan, T. Kemala Alferraly, T. Ibnu Betty, Betty Soekimin, Soekimin Delyuzar, Delyuzar ABSTRACT Background:Penile squamous cell carcinoma is the  most common penile malignance found in the world. Assessment of  stromal tumor infiltrating lymphocytes (STILs) associated with grading against  penile squamous cell carcinoma can provide  a potential prognostic picture  and help determine the action. Method:Cross-sectional  analytical study  with 32 samples of histopathological preparations diagnosed as  penile squamous cell carcinoma  at H. Adam Malik Hospital Medan. The assessment of stromal TILs was carried out by assessing the number of lymphocyte inflammatory cells in  the peritumoral stroma and then determined the assessment score, zero score no lymphocytes (minimum), score one found 0-10% lymphocytes (moderately), score two found 20-40% lymphocytes  (massive) , score three found 50-90% lymphocytes. Grading assessments are categorized into  grade  I (well differentiated), grade  II (moderately differentiated), and  grade III  (poorly  differentiated).   Result:The most grading found was grade II (43.8%) and the most STILs found were score three (50.0%). There is a significant relationship between grading and  STILs in  penile squamous cell carcinoma  (p value=0.0001) i.e. the higher  the STILs score  , the lower  the tumor grade. Conclusion:There is a relationship between STILs and  histopathological grading in Penile Squamous Cell Carcinoma  so that it can be used as an indikasi prognosis in patients with  penile squamous cell carcinoma.     Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2024-09-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/580 10.55816/mpi.v33i2.580 Majalah Patologi Indonesia; Vol. 33 No. 2, Mei 2024 2527-9106 0215-7284 10.55816/mpi.v33i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/580/434 Copyright (c) 2024 Marisi Cintya Debby Sihotang https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/581 2025-12-19T17:03:15Z patologi:ART driver

High Expression of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 Correlates with High Grade of Clear Cell Renal Cell Carcinoma Rezeki, Dewi Sri Laksmi, Lidya Imelda Mariedina, Causa Trisna Alferraly, T. Ibnu Lukito, Joko S. Betty, Betty clear cell renal cell carcinoma ISUP grading nicotinamide adenine dinucleotide phosphate oxidase 4 Background Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype in renal cell tumors (RCT). This is a very aggressive cancer, often diagnosed at advanced stage leading to increase morbidity and mortality rates. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 4 (NOX-4) expression plays role in the regulation of several cancer types. The expression of NOX-4 is associated with a poorer degree of differentiation and a shorter survival in RCT. Therefore, NOX-4 expression is predictor of prognosis and potential therapeutic target. Research on the correlation of NOX-4 expression in RCTs is still very limited, while Correlation of NOX-4 expression in ccRCC does not exist yet.   Objective To assess the relationship between NOX-4 expression and ISUP grading in ccRCC.   Method In this study, 30 slide samples and ccRCC paraffin blocks were diagnosed histopathologically at Department of Anatomic Pathology of H. Adam Malik General Hospital from January 2012 to April 2022. All clinicopathological data were taken from medical records/pathology archives. The expression of NOX-4 immunohistochemical was assessed in nucleus and cytoplasm of tumor cells. The assessment was carried out semiquantitatively based on intensity and percentage of expressed tumor cells. The relationship between NOX-4 expression and ISUP grading was assessed using Fisher's exact test.   Results Of the 30 ccRCC samples, high grade ccRCC was found in 21 cases (70%) and strong expression of NOX-4 in cytoplasm and nucleus in 20 cases (66.7%). Strong expression of NOX-4 in high grade ccRCC was found in 17 cases (81.0%). A significant relationship was observed between NOX-4 expression and ISUP grading on ccRCC with a p-value of 0.030 (p<0.05) using Fisher's exact test.   Conclusion There is a significant relationship between NOX-4 expression and ISUP grading in ccRCC. Therefore, NOX-4 expression can be used for the prognosis of ccRCC and therapy development. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2024-06-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/581 10.55816/mpi.v33i2.581 Majalah Patologi Indonesia; Vol. 33 No. 2, Mei 2024 2527-9106 0215-7284 10.55816/mpi.v33i2 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/581/429 Copyright (c) 2024 Dewi Sri Rezeki, Lidya Imelda Laksmi, Causa Trisna Mariedina, T. Ibnu Alferraly, Joko S. Lukito, Betty Betty https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/584 2025-12-19T17:01:42Z patologi:ART driver

Correlation between Reactive Stromal Expression and Histochemical Trichrome Masson Staining with Histopathological Grading in Prostate Adenocarcinoma at Adam Malik Haji Center General Hospital Medan Basrul, Mohd. Yuwanda Laksmi, Lidya Imelda Mariedina, Causa Trisna Lubis, M. Nadjib Dahlan Intan, T. Kemala Chrestella, Jessy Prostate adenocarcinoma reactive stromal expression gleason score trichrome masson Background Adenocarcinoma of the prostate is an invasive carcinoma, composed of neoplastic prostatic epithelial cells with differentiation and secretion arranged in various histomorphological patterns.Quantitative analysis of the elements that make up the stroma in casesprostate adenocarcinomahas been linked to prognostic factors and the Gleason score, which correlates with progression and metastasis, and may contribute to new prognostic feature approaches.   Method Descriptive analytic study with 33 paraffin block samples diagnosed as prostate adenocarcinoma at Haji Adam Malik General Hospital Medan.Assessment is done forsee the relationship between reactive stromal expression using Trichrome Masson and histopathological grading gleason score then determine the scoring score for the stroma around the prostate gland adenocarcinoma lesion stained blue with trichrome masson, a positive score of one (+): if 0-15% stroma is stained blue, positive two (++): if >15%-<50% stroma is stained and triple positive (+++): if >50% stroma is stained blue. The histopathological grading was categorized into grade I (well differentiated: Gleason score ≤6), grade II (moderately differentiated: Gleason score 7 (3+4)), and grade III (poorly differentiated: Gleason score 7 (4+3) and 8 -10).   Results: Reactive stromal expression the most found was positive three (+++) (69.7%) and the most common histopathological grading was grade III (69.7%). There was a significant correlation between reactive stromal expression and histopathological grading in prostate adenocarcinoma (p-value=0.044), namely the worse the cell differentiation, the higher the positive score of reactive stromal expression.   Conclusion Assessment of reactive stromal expression associated with histopathological grading can be used as an indication of prognosis in patients with prostate adenocarcinoma. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2025-01-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/584 10.55816/mpi.v33i3.584 Majalah Patologi Indonesia; Vol. 33 No. 3, September 2024 2527-9106 0215-7284 10.55816/mpi.v33i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/584/436 Copyright (c) 2025 Mohd. Yuwanda Basrul, Lidya Imelda Laksmi, Causa Trisna Mariedina, M. Nadjib Dahlan Lubis, T. Kemala Intan, Jessy Chrestella https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/585 2025-12-19T17:04:13Z patologi:ART driver

Analysis of H3k27me3 Expression in Malignant Peripheral Nerve Sheath Tumor (MPNST) and Other Spindle Cell Sarcoma Mimicking MPNST Yordana, William Evelina, Evelina Kodariah, Ria MPNST, non-MPNST, H3K27me3. Malignant Peripheral Nerve Sheath Tumor (MPNST) is a type of spindle cell sarcoma with approximately 5% of all sarcomas. Its diagnosis is challenging due to the absence of specific immunohistochemical markers. Recently, H3K27me3 was discovered as a potential specific immunohistochemical marker to differentiate MPNST from other sarcomas and distinguish between low and high-grade MPNST. Therefore, this research aims to investigate the use of the H3K27me3 as a potential specific marker for Malignant Peripheral Nerve Sheath Tumor (MPNST). A cross-sectional analysis was conducted on 50 cases of sarcomas, including 13 MPNST, 14 synovial sarcomas, 13 dermatofibrosarcoma protuberans (DFSP), and 10 leiomyosarcomas originating from the Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital (FMUI-CMH) from January 2013 to December 2021. H3K27me3 images were obtained and categorized as complete loss when more than 95% of the tumor cells showed loss of nuclear staining. The results found in MPNST showed a loss of H3K27me3 expression, which is statistically significant compared to other sarcomas mimicking MPNST (p=0.021), indicating its potential as a diagnostic marker. There is a difference in the expression of H3K27me3 between the high and low-grade MPNST but it is not statistically significant (p=0.105). This showed that H3K27me3 loss of expression can be used to diagnose MPNST, especially high-grade MPNST, and differentiate it from other sarcomas mimicking MPNST. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2023-10-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/585 10.55816/mpi.v32i3.585 Majalah Patologi Indonesia; Vol. 32 No. 3, September 2023 2527-9106 0215-7284 10.55816/mpi.v32i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/585/411 Copyright (c) 2023 Majalah Patologi Indonesia https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/596 2025-12-19T17:01:42Z patologi:ART driver

Clinicopathological Characteristics of Salivary Gland Mucoepidermoid Carcinoma In dr Hasan Sadikin Central Hospital (RSHS) Bandung: A 10 Year review Karakteristik klinikopatologikal Mucoepidermoid Carcinoma kelenjar liur di Rumah Sakit Umum Pusat dr Hasan Sadikin (RSHS) Bandung tahun 2013-2022: A 10 Year review Noor Fitriana, Maya Usman, Hermin Aminah Yantisetiasti, Anglita Background Primary salivary gland malignancy is rare and has limited reports. Mucoepidermoid Carcinoma (MEC) is the most common malignant tumor of the salivary glands. However, epidemiological reports on salivary gland MEC are still limited, particularly in Indonesia. This study aimed to report the clinicopathological characteristics of Salivary gland MEC in dr Hasan Sadikin Central Hospital (RSHS) from January 2013 to December 2022. Method: This study is a descriptive study with a cross-sectional method. Total cases of salivary glands MEC that were histopathologically diagnosed and recorded in the RSHS Hospital Information System (SIRS) medical records from 2013 to 2022 were collected. It is included demographic, clinical, and histopathological data. Result A total of 86 MEC cases were diagnosed histopathologically between 2013 and 2022. The cases involved 44 (51,2%) females and 42 (48,8%) males. The mean age is 46,4 years old. The most common site affected is a parotid gland (37,2%). According histopathological grade, 51,2% were high grade, 25,6% were intermediate grade, and 23,3% were low grade. The result of clinical stage is Stage III has the highest percentage (n=32, 37,2%). The majority of patients receive surgical combined with radiotherapy treatment (n=53; 61,6%). Conclusion There are 86 cases of Salivary MEC in RSHS period 2013-2022. MEC is common in females, mean age 46,4 years old. Parotid gland is the most common site. The most frequent histopathological grading and clinical staging are high grade and stage III. Keyword Mucoepidermoid carcinoma, salivary gland, clinicopathological, RSHS Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2024-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/596 10.55816/mpi.v33i2.596 Majalah Patologi Indonesia; Vol. 33 No. 3, September 2024 2527-9106 0215-7284 10.55816/mpi.v33i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/596/438 Copyright (c) 2024 Maya Noor Fitriana, Hermin Aminah Usman, Anglita Yantisetiasti https://creativecommons.org/licenses/by-nc-nd/4.0

oai:ojs.pkp.sfu.ca:article/597 2025-12-19T17:04:13Z patologi:ART driver

Correlation of CD133 Expression with Tumor Location and Lymph Node Involvement in Colorectal Carcinoma English Adelia Rachmita, Prima Hilbertina, Noza Yenita, Yenita Agus, Salmiah Suchitra, Avit Yetti, Husna English CD133 expression colorectal carcinoma lymph node involvement tumor location Introduction Colorectal carcinoma (CRC) is the third most common cancer worldwide and the fourth most common cause of cancer-related death. Ongoing efforts to investigate new treatment strategies for CRC include understanding CSC (Cancer Stem Cells) involvement. CSC is a group of tumor cells with stem cell characteristics that can play a role in metastasis, recurrence, and therapeutic resistance. This study aims to determine the correlation of CD133 expression as a CSC marker in CRC with tumor location and lymph node involvement in CRC. Methods This research is a cross-sectional study. The research sample obtained as many as 50 cases of paraffin blocks originating from resection. Immunohistochemical staining was performed to assess CD133 expression. Chi-square test statistical analysis was carried out with a p-value <0.05, considered significant. Results This study found that CD133 expression was higher in tumors located in the rectum compared to distal and proximal colon, 56%, 28%, and 16%, respectively. Statistically, there is a significant correlation between CD133 expression and lymph node involvement in CRC (p-value = 0.048). Conclusions There is a significant correlation between CD133 expression and lymph node involvement in CRC. Unfortunately, there was no statistically significant correlation between CD133 expression and tumor location. More extensive studies are needed to establish CD133 as a test performed on every patient with CRC. Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA) 2023-10-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/597 10.55816/mpi.v32i3.597 Majalah Patologi Indonesia; Vol. 32 No. 3, September 2023 2527-9106 0215-7284 10.55816/mpi.v32i3 eng http://majalahpatologiindonesia.com/p/index.php/patologi/article/view/597/412 Copyright (c) 2023 Majalah Patologi Indonesia https://creativecommons.org/licenses/by-nc-nd/4.0 4e6c4e58bb31a786ff4d035d5d333a8f