Identification of CYP1A1 Gene Polymorphism in Squamous Cell Carcinoma and Cervical Adenocarcinoma

Abstract

Background
Cervical cancer is the third most common cancer in women with risk factor of smoking, high parity, long term use of oral
contaception that are associated with chemical carcinogenesis. Chemical carcinogenesis require biotransfor-mation of lipophilic
substrates to hydrophilic metabolites, therefore facilitating their secretion from the human body. Cytochrome P450 (CYP) is one of
genes that have important role in this process. Benzo[α]pyrene and estrogen have a common biotransformation process which is
metabolized by CYP, particularly CYP1A1. The objectives to identify the frequency and distribution of CYP1A1 gene polymorphism
in squamous cell carcinoma and adenocarcinoma of the cervix.
Methods
This is an analytical descriptive study with cross sectional approach. CYP1A1 gene polymorphism (3801T/C or Ile462Val) was
analyzed using PCR-RFLP method followed by gel electrophoresis.
Results
CYP1A1 gene polymorphisms (3801TC) in squamous cell carcinoma were 50% heterozygote T/C, 36% wild-types T/T and 14%
homozygote C/C. CYP1A1 gene polymorphisms (3801TC) in adenocarcinoma were 60% heterozygote T/C and 40% wild-types T/T.
CYP1A1 gene polymorphisms (Ile462Val) in squamous cell carcinoma were 97.2% heterozygote Ile/Val, and 2.8% homozygote
Val/Val. CYP1A1 gene polymorphisms (Ile462Val) in adenocarcinoma were 100% heterozygote Ile/Val
Conclusion
The most common type of CYP1A1 gene polymorphism (3801TC and Ile462Val) in squamous cell carcinoma and adenocarcinoma
of the cervix were heterozygote.