Relationship of CD-68 Associated Macrophages Tumor Density and Clinicopathological Characteristics in Invasive Breast Carcinoma

Abstract

Background
Breast carcinoma is the most common malignancy among women worldwide. Many factors can influence prognosis of the disease.
Tumor microenvironment (TME) is enriched in highly active immune cells. Herein, tumor associated macrophages (TAM) are the
most prevalent immune cells. TAM can enhance tumor progression by promoting angiogenesis, invasion and metastasis. Some
studies demonstrate that high density of macrophages infiltration associated with poor prognosis. The aim of the study is to
determine the association between TAM density and clinicopathological characteristics of breast invasive carcinoma.
Methods
Descriptive study with case series design on January 1, 2015-October 31, 2017 was performed at Department of Anatomical
Pathology Faculty of Medicine University of Sriwijaya/dr. Moh. Hoesin General Hospital Palembang on 48 samples invasive
carcinoma mammae subtype unspecific, and have already staining with immunohistochemistry staining ER, PR, HER-2 and Ki-67,
surrogate markers. Then immunohistochemistry staining using CD-68 antibody was conduced. The association between TAM
density and clinicopathological characteristics were analyzed by using Spearman’s Rho test.
Results
The majority of the patient are older than 40 years of age (77.1%), with positive lymphovascular invasion (79.2%), and at the grade
III (62.5%). Bivariate analysis between TAM CD-68+
density in stromal tumor and subtype luminal A showed negative correlation
moderate significantly (r= -0.398; p=0.005) while between TAM CD-68+
density in stromal tumor and subtype triple negative showed
positive correlation moderate significantly (r=0.335; p=0.020).
Conclusion
TAM CD-68+
density in stromal tumor was significantly correlated with luminal A and triple negative subtype.