Ekspresi Protein Ki-67 pada Biopsi Kolonoskopi Adenoma dan Karsinoma Kolorektal

Abstract

Background Neoplastic lesion in colorectal could be adenoma and colorectal carcinoma (CRC). An invasion in muscularis mucosa is a criteria for colorectal carcinoma. Diagnosis is formed with histopatologic interpretation. In colorectal-biopsy preparation interpretation, determining limited invasion is sometimes difficult so that it needs other diagnostic tools. The aim of this study was to evaluating the difference of Ki-67 mean expression in colorectal adenoma and CRC. Methods This study was cross-sectional study on colorectal adenoma and CRC. Cases were diagnosed by colonoscopy-biopsy between 2011 and 2012. It did re-interpretation of HE preparation of adenoma or colorectal carcinoma diagnosis and determination of the degree of differentiation of those adenoma and colorectal carcinoma. Immunohistochemical Ki-67 staining was interpreted with light microscope. The percentage of the stained tumour cells was counted. The difference of mean in Ki-67 expression of colorectal adenoma and CRC was analyzed with t-test with significance level at p<0.05. Results The mean of Ki-67 expression in colorectal carcinoma was higher compared to adenoma (p= 0.000). t test of Ki-67 expression was significantly difference in colorectal carcinomas between low and high grade (p=0.018). On low and high grade adenoma, the Ki-67 expression was no significantly difference (p=0.110). Conclusion Ki-67 expression could be used as a basic to confirm conventional diagnostic of adenoma and colorectal carcinoma. Key words: adenoma, colonoscopy biopsy, colorectal carcinoma, Ki-67.