The Relationships Between P16INK4a Immunohistochemistry Expression With Subtypes And Histopathological Grading In Squamous Cell Carcinoma Of The Penis.

Abstract

ABSTRACT

Background:

Penile squamous cell carcinoma is a malignancy of the epithelial cells of the prepuce or glans penis, occupying 95% of all malignancies in the penis.The average patient age is 50 until 70 years. The p16^INK4a immunohistochemistry is a cyclin dependent kinase inhibitor and can be expressed by human papilloma virus. Several studies have tried to relate this expression to eradicate this virus. 

Methods:

This study use cross-sectional analytical study with 30 samples of histopathological preparations diagnosed as penile squamous cell carcinoma at H. Adam Malik Hospital Medan. Assessment of p16^INK4a expression was made based on analysis of the percentage of positive tumor cells and staining intensity. Score 0: (no positive tumor cells). Score +1: (≤10% positive tumor cells). Score +2: (11–50% positive tumor cells). Score +3: (> 50% positive tumor cells). The color intensity of p16^INK4a  is categorized as follows:  Score 0: (negative). Score 1: (weak). Score 2: (strong).

Results:

The most common age was 40-49 years, the most location was in more than one region, the most subtype was HPV-associated ,the most weak positive expression was the HPV-associated subtype and the most strong positive expression was HPV-associated of squamous cell carcinoma of penile. The highest immunohistochemical expression of p16^INK4a  was weakly positive at grade 3.

Conclusion:

There is a significant relationship between p16^INK4a and histopathological subtypes, but there was no significant with the grading of penile squamous cell carcinoma.