Hubungan Jumlah Sel Mast dengan Tampilan Ekspresi Vascular Endothelial Growth Factor (VEGF) pada Cutaneous Squamous Cell Carcinoma

Abstract

Background
The most common non-melanocytic skin cancers are basalioma and squamous cell carcinoma. Squamous
cell carcinoma is a malignancy of squamous epithelium. Mast cells stimulate neovascularization and
angiogenesis in multiple tumors. One of the main factors of angiogenesis in squamous cell carcinoma is
the Vascular Endothelial Growth Factor (VEGF). The aim of this study is to investigate the association
between number of mast cells with the expression of VEGF of cutaneous squamous cell carcinoma.
Methods
This is an analytical descriptive study with cross sectional approach. The sample of this study are paraffin
blocks of skin tissue which diagnose histopathologically as squamous cell carcinoma. Then the samples
will be stained with immunohistochemistry CD117 (Novocastra®) to count the number of mast cells and
VEGF (Dako®) to see angiogenesis. Their expression will be analyzed with Fisher’s Exact Test statistical
analysis to investigate the association between number of mast cells with expression of VEGF of
cutaneous squamous cell carcinoma.
Results
The number of mast cells ≥15 cells were 7 cases (23.3%). Expressed VEGF positive expression in 30
cases (100%). Suitability test Fischer's Exact Test has value not significant (p> 0.05).
Conclusion
There is no conformance relationship between the number of mast cells with VEGF expression of p-value.